Dual tagging of GFP and Degron on endogenous COSA-1 in Caenorhabditis elegans as a crossover investigation tool [0.03%]
在Caenorhabditis elegans中对内源性的COSA-1进行GFP和Degron的双标记作为一种交叉研究工具
Arome Solomon Odiba,Guiyan Liao,Haiyan Yuan et al.
Arome Solomon Odiba et al.
COSA-1 is essential for accurate meiosis in C. elegans . Two null mutants ( cosa-1 ( me13 ) and cosa-1 ( tm3298 ) ) have been notably studied. These null mutants exhibit severe meiotic defects, hindering the observation of the subtle or dyn...
Mizuki Sakamoto,Takashi Ishiuchi
Mizuki Sakamoto
YY1 plays multifaceted roles in various cell types. We recently reported that YY1 regulates nucleosome organization in early mouse embryos. However, despite the impaired nucleosome organization in the absence of YY1, the transcriptome was m...
Immobilization of C. elegans with different concentrations of an anesthetic for time-lapse imaging of dynamic protein trafficking in neurons [0.03%]
使用不同浓度的麻醉剂固定线虫以进行长时间拍摄神经元中动态蛋白运输的过程
Cesar Siete,Rui Xiong,Anaam Khalid et al.
Cesar Siete et al.
Here we compare the percentage of anterograde and retrograde trafficking events as well as the average velocity of these events in worms immobilized with microbeads or 0.5-7.5 mM tetramisole. Our results show that the percentage and average...
Erratum: Corrigendum: First report of Tarantula-parasitic nematode Tarantobelus jeffdanielsi from Los Angeles, California [0.03%]
勘误：首次报道来自加利福尼亚州洛杉矶的球鼠毒蜘蛛寄生线虫Tarantobelus jeffdanielsi 的更正说明
[This corrects the article DOI: 10.17912/micropub.biology.000825.]. Copyright: © 2024 by the authors.
Clearance of extracellular human amyloid-β aggregates in C. elegans by nutraceutical and pharmaceutical interventions [0.03%]
营养补充剂和药物干预清除线虫中外源性人类淀粉样蛋白-beta集聚块
Arastu Sharma,Collin Y Ewald
Arastu Sharma
Numerous anti-amyloid therapies have seen recent clinical development and approval, such as the monoclonal antibodies aducanumab and lecanemab. However, in Alzheimer's disease patients, amyloid-β (Aβ) plaques are found embedded in the ext...
NALCN Channels Are Not Major targets of Gα o or Gα q Modulation in the C. elegans Egg-Laying Behavior Circuit [0.03%]
NALCN通道不是Gα o或Gα q在C. elegans产卵行为回路中调节的主要目标
Ariana Jose,Kevin Collins
Ariana Jose
Sodium leak channels (NALCN) are regulators of cell membrane potential. Previous studies in mammalian neurons and C. elegans have shown that Gα q and Gα o signaling antagonistically modulates NALCN activity to regulate neuron excitability...
Sara Medina-Suárez,Félix Machín
Sara Medina-Suárez
CRISPR/Cas9 gene editing technology has revolutionized genetic engineering. However, the nuclear dynamics of Cas9 in eukaryotic cells, particularly in the model organism Saccharomyces cerevisiae , remains poorly understood. Here, we constru...
Fission yeast spindle dynamics and chromosome segregation fidelity show distinct thermosensitivity [0.03%]
裂殖酵母纺锤体动态和染色体分离保真度显示出不同的热敏感性
Zachary Chaba,Ishutesh Jain,Phong T Tran
Zachary Chaba
Cellular processes rely on proteins with temperature-dependent stability and activity. While thermosensitivity in biological networks is well-explored, the effect of temperature on complex mechanochemical assemblies, like the spindle, is ra...
Remote homology identification of the Drosophila melanogaster ortholog of the RNA Polymerase I subunit Rpa34/POLR1G [0.03%]
黑腹果蝇RNA聚合酶I亚基Rpa34 / POLR1G的远程同源识别
Ryan Palumbo,Bruce Knutson
Ryan Palumbo
Highly conserved orthologous proteins are easily identified by sequence homology alone, whereas poorly conserved orthologs require additional structural information to be identified. All Drosophila orthologs of RNA polymerase I, II, and III...
Peach LAZY1 and DRO1 protein-protein interactions and co-expression with PRAF/RLD family support conserved gravity-related protein interactions across plants [0.03%]
桃LAZY1和DRO1的蛋白-蛋白相互作用以及与PRAF/RLD家族的共表达支持植物中保守的与重力相关的蛋白质相互作用
Jessica M Waite,Courtney A Hollender,Jon R Eilers et al.
Jessica M Waite et al.
IGT/LAZY proteins play a central role in determining gravitropic set point angle and orientation of lateral organs across plant species. Recent work in model systems has demonstrated that interactions between IGT/LAZY proteins and BREVIS RA...