Characterizing a standardized BioPart for PVQ-specific expression in C. elegans [0.03%]
用于秀丽隐杆线虫PVQ特异性表达的BioPart的标准表征
Sarah AlHarbi,Christian Frøkjær-Jensen
Sarah AlHarbi
Synthetic biology relies on standardized biological parts (BioParts), and we aim to identify cell-specific promoters for every class of neuron in C. elegans . Here, we characterize a short BioPart (P nlp-17 , 300 bp) for PVQ-specific expres...
Wolbachia genetically interacts with the bag of marbles germline stem cell gene in male D. melanogaster [0.03%]
沃尔巴克氏体与男性的生殖干细胞基因bag of marbles发生基因互作
Miwa Wenzel,Charles F Aquadro
Miwa Wenzel
The bacterial endosymbiont Wolbachia manipulates reproduction of its arthropod hosts to promote its own maternal vertical transmission. In female D. melanogaster , Wolbachia has been shown to genetically interact with three key reproductive...
Comparison of three DNA extraction methods for recovery of microbial DNA from Arctic permafrost [0.03%]
三种用于从北极永冻土中提取微生物DNA的方法的比较
Sarah Feng,Marla DeKlotz,Neslihan Taş
Sarah Feng
Permafrost soils, which contain one of Earth's largest terrestrial carbon stocks, are vulnerable to thaw and microbial decomposition, exacerbating climate change. Advancements in sequencing technologies have facilitated the identification a...
Chromosomal fusions, but not chromosomal inversions, activate a PCH-2 dependent checkpoint that promotes crossover formation in C. elegans [0.03%]
染色体融合而非染色体重排激活了C. elegans中的一个PCH-2依赖性检查点以促进互换形成
Bhumil Patel,Maryke Grobler,Needhi Bhalla
Bhumil Patel
Meiotic crossovers promote accurate chromosome segregation during gametogenesis. In C. elegans , a highly conserved AAA ATPase, PCH-2, ensures that homologous chromosomes have at least one crossover, preventing meiotic defects. PCH-2 locali...
The Architect of Neurotransmission in C. elegans : How FLP-3 Neuropeptides' Structures Direct their Function [0.03%]
秀丽隐杆线虫神经递质的构建者：FLP-3神经肽结构如何决定其功能
Rehab Salama,Elizabeth DiLoreto,Jagan Srinivasan
Rehab Salama
Neuropeptides direct functions in the nervous, endocrine, and immune systems of all animals by altering the activity at neural synapses. A single neuropeptide gene can be post-translationally modified to create multiple active peptides. The...
Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva [0.03%]
线虫生殖表皮中肌动蛋白-骨架蛋白复合物极性分布及其调节机制研究进展
Trevor J Barker,Fung-Yi Chan,Ana X Carvalho et al.
Trevor J Barker et al.
The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is ...
Characterization of mechanosensitive MSL gene family expression in Zea mays aerial and subterranean brace roots [0.03%]
MSL机械敏感基因家族在玉米须根和定根中的表达特征分析
Olivia S Hazelwood,Ashley N Hostetler,Irene I Ikiriko et al.
Olivia S Hazelwood et al.
Plants must be able to sense and respond to mechanical stresses encountered throughout their lifespan. The MscS-Like (MSL) family of mechanosensitive ion channels is one mechanism to perceive mechanical stresses. In maize, brace roots emerg...
Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila [0.03%]
利用AGES（Auxin诱导的基因表达系统）和TARGET（时间和区域性的基因表达靶向）系统在果蝇中对神经元基因表达进行微调的比较以及神经元GAL4驱动子的评估
Hannah R Hawley,Celestine J Roberts,Helen L Fitzsimons
Hannah R Hawley
Spatial and temporal control of gene expression in Drosophila is essential in elucidating gene function. Spatial control is facilitated by the UAS/GAL4 system, and this can be coupled with additional adaptations for precise temporal control...
mScarlet and split fluorophore mScarlet resources for plasmid-based CRISPR/Cas9 knock-in in C. elegans [0.03%]
用于秀丽隐杆线虫基于质粒的CRISPR / Cas9插入的mScarlet和分裂荧光蛋白mScarlet资源
Gillian Witten,Ella DeMott,George Huang et al.
Gillian Witten et al.
Fluorescent proteins allow the expression of a gene and the behavior of its protein product to be observed in living animals. The ability to create endogenous fluorescent protein tags via CRISPR genome engineering has revolutionized the aut...
frpr-18, a neuropeptide receptor, regulates organismal lifespan and stress tolerance in C. elegans [0.03%]
C．伊氏线虫中神经肽受体frpr-18调节生物体寿命和应激耐受性
Hafsa Ouaakki,Heetanshi Joshi,Laxmi Rathor et al.
Hafsa Ouaakki et al.
The mechanisms underlying neuropeptide signaling regulation of lifespan in Caenorhabditis elegans ( C. elegans ) remain unclear. FRPR-18 is a mammalian orexin/hypocretin-like receptor and modulates C. elegans arousal behavior by acting as a...