Development of pXeDNA3.1-LacZ, a Dual-System Plasmid for Membrane Protein Expression in Xenopus and Mammalian Cells [0.03%]
非洲爪蟾和哺乳动物细胞膜蛋白表达的双系统质粒pXeDNA3.1-LacZ的构建
Li Guan,Tom E Reynoldson,Eleonora M Pieroni et al.
Li Guan et al.
Cross-species expression of proteins in mammalian cells and Xenopus oocytes remains a powerful strategy for functional studies. This can be facilitated by plasmids with dual promoters, enabling use in both systems without separate subclonin...
Genome sequence of bacteriophage AmiCi24 isolated using Arthrobacter globiformis [0.03%]
利用色杆菌（Arthrobacter globiformis）分离的噬菌体Amici24基因组序列
Paige Baldwin,Michael Bennett,Evangelia Buonamici et al.
Paige Baldwin et al.
AmiCi24 is a novel siphoviral bacteriophage that was isolated from a soil sample collected in Sewell, NJ, USA using Arthrobacter globiformis B-2979 as the host. AmiCi24 has a genome consisting of 38,466 base pairs that encodes 68 predicted ...
A robust absolute quantification protocol for potato virus Y based on reverse transcription-quantitative PCR [0.03%]
基于反转录定量PCR的马铃薯Y病毒抗血性定量方法
Diana Spencer,Elizabeth Padilla-Crespo,Ek Han Tan
Diana Spencer
Standardized protocols for absolute quantification of potato virus Y (PVY) from potato tissue is critical for host-virus dynamic studies. Here, we developed a standardized protocol using a cloned viral sequence as standards to detect and qu...
Akane Iizuka,Naotaka Nakazawa
Akane Iizuka
Aggressive cell migration is a hallmark of cancer cells. Cancer cells pass through a 3D confined environment during metastasis, which induces mechanical stress on the nucleus. Transwell membranes have been used in research to induce mechani...
Automated detection of spontaneous calcium signaling events in prohemocytes of the Drosophila melanogaster lymph gland [0.03%]
果蝇淋巴器官中前血细胞自发钙信号事件的自动化检测方法
Xinwen Zhu,Max V E Smith,Guy Tanentzapf
Xinwen Zhu
Calcium signaling is an important regulator of stem cell maintenance and differentiation. Here we report the development of an image processing pipeline for ex vivo time-lapse microscopy data that enables the unbiased, automated detection o...
Loss of uterine EGR2 contributes to age-associated decline in fertility in female mice [0.03%]
小鼠子宫EGR2的丧失导致其生育能力随着年龄的增长而下降
Sudikshya Paudel,Magdalina J Cummings,Steven L Young et al.
Sudikshya Paudel et al.
Early growth response 2 (Egr2) is a pleiotropic zinc finger transcription factor with established roles in neural and immune system, but its uterine function remains poorly understood. We found that uterine EGR2 is expressed in luminal epit...
Long multiply marked DNA repair template reveals lengths and fidelity of genome editing tracts in Schizosaccharomyces pombe [0.03%]
长标记DNA修复模板揭示了模式生物S. pombe中基因组编辑片段的长度和保真度
Reine U Protacio,Nyera A Ali,Akshara Chevireddy et al.
Reine U Protacio et al.
To test the ability of a fission yeast CRISPR-Cas9 system ( SpEDIT ) to carry out genome editing over distance, we constructed a 1,935 bp-long, dsDNA repair template that contained 45 base pair substitutions (SNPs), relative to the wild-typ...
Yu Matsumoto,Ban Sato,Masafumi Inui et al.
Yu Matsumoto et al.
After ejaculation, the intrauterine environment undergoes dynamic fluid changes due to post-ejaculated uterine fluid (eUF) coagulation and subsequent liquefaction. These changes presumably contribute to fertilization and reproductive effici...
Patrick Erickson,Alexandra Burr,Biju Parekkadan
Patrick Erickson
The promise of adeno-associated virus (AAV) vectors for gene therapy is held back by the cost and toxicity of the large doses required. This experiment explored the chronopharmacology of AAVs in mice by studying how circadian phase at the t...
Opposite aging effects among cell subsets revealed in the human transcriptome and epigenome [0.03%]
人类转录组和表观基因组中揭示了细胞亚群中相反的衰老效应
Daigo Okada
Daigo Okada
In our previous work, we reported a global landscape of opposite aging effects among mouse cell subsets, where each cell subset is defined as a combination of tissue and cell type, and aging leads to increased gene expression in one subset ...