Long multiply marked DNA repair template reveals lengths and fidelity of genome editing tracts in Schizosaccharomyces pombe
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To test the ability of a fission yeast CRISPR-Cas9 system ( SpEDIT ) to carry out genome editing over distance, we constructed a 1,935 bp-long, dsDNA repair template that contained 45 base pair substitutions (SNPs), relative to the wild-type target locus ade6 ... ...
