Anuska Das,Jay Rai,Mitchell O Roth et al.
Anuska Das et al.
Controlling the activity of the CRISPR-Cas9 system is essential to its safe adoption for clinical and research applications. Although the conformational dynamics of Cas9 are known to control its enzymatic activity, details of how Cas9 influ...
Product analog binding identifies the copper active site of particulate methane monooxygenase [0.03%]
颗粒甲烷单加氧酶的铜活性位点的产品类似物结合鉴定
Frank J Tucci,Richard J Jodts,Brian M Hoffman et al.
Frank J Tucci et al.
Nature's primary methane-oxidizing enzyme, the membrane-bound particulate methane monooxygenase (pMMO), catalyzes the oxidation of methane to methanol. pMMO activity requires copper, and decades of structural and spectroscopic studies have ...
The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis [0.03%]
谷胱甘肽转移酶在异源花青素生物合成中的催化作用
Michael Eichenberger,Thomas Schwander,Sean Hüppi et al.
Michael Eichenberger et al.
Anthocyanins are ubiquitous plant pigments used in a variety of technological applications. Yet, after over a century of research, the penultimate biosynthetic step to anthocyanidins attributed to the action of leucoanthocyanidin dioxygenas...
Robin Girod,Timon Lazaridis,Hubert A Gasteiger et al.
Robin Girod et al.
Catalyst layers in proton exchange membrane fuel cells consist of platinum-group-metal nanocatalysts supported on carbon aggregates, forming a porous structure through which an ionomer network percolates. The local structural character of t...
Sayan Kar,Michael Rauch,Gregory Leitus et al.
Sayan Kar et al.
Formic acid (FA) is a promising hydrogen carrier which can play an instrumental role in the overall implementation of a hydrogen economy. In this regard, it is important to generate H2 gas from neat FA without any solvent/additive, for whic...
Rui Guo,Heyu Chen,Yang Yang
Rui Guo
General catalytic methods for free radical-mediated asymmetric transformations have long eluded synthetic organic chemists. Now, NAD(P)H-dependent ketoreductases (KREDs) are repurposed and engineered as highly efficient photoenzymes to cata...
Chemodivergent C(sp3)-H and C(sp2)-H Cyanomethylation Using Engineered Carbene Transferases [0.03%]
工程化卡宾转移酶介导的化学分异C(sp3)—H和C(sp2)—H氰甲基化反应
Juner Zhang,Ailiena O Maggiolo,Edwin Alfonzo et al.
Juner Zhang et al.
The ubiquity of C-H bonds presents an attractive opportunity to elaborate and build complexity in organic molecules. Methods for selective functionalization, however, often must differentiate among multiple chemically similar and, in some c...
Maximizing light-driven CO2 and N2 fixation efficiency in quantum dot-bacteria hybrids [0.03%]
量子点-细菌杂化体系中光驱动CO2和N2固定的效率最大化研究
Xun Guan,Sevcan Erşan,Xiangchen Hu et al.
Xun Guan et al.
Integrating light-harvesting materials with microbial biochemistry is a viable approach to produce chemicals with high efficiency from the air, water, and sunlight. Yet it remains unclear whether all absorbed photons in the materials can be...
Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR-Cas9 [0.03%]
CRISPR-Cas9靶DNA切割原则及Mg2+催化作用
Łukasz Nierzwicki,Kyle W East,Jonas M Binz et al.
Łukasz Nierzwicki et al.
At the core of the CRISPR-Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorating Cas9 function is still missing. Here, multi-microsecond molecul...
Molecular mechanism of topoisomerase poisoning by the peptide antibiotic albicidin [0.03%]
アルバシ丁によるトポイソメラーゼ毒性作用の分子機構
Elizabeth Michalczyk,Kay Hommernick,Iraj Behroz et al.
Elizabeth Michalczyk et al.
The peptide antibiotic albicidin is a DNA topoisomerase inhibitor with low-nanomolar bactericidal activity towards fluoroquinolone-resistant Gram-negative pathogens. However, its mode of action is poorly understood. We determined a 2.6 Å r...