Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR-Cas9
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At the core of the CRISPR-Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorating Cas9 function is still missing. Here, multi-microsecond molecular dynamics, free-en... ...
