Probing Exchange Units for Combining Iterative and Linear Fungal Nonribosomal Peptide Synthetases [0.03%]
探究交换单位在结合迭代和线性真菌非核糖体肽合成酶方面的应用
Charlotte Steiniger,Sylvester Hoffmann,Roderich D Süssmuth
Charlotte Steiniger
A considerable number of complex peptides are synthesized by nonribosomal peptide synthetases (NRPSs). Due to their multimodular architecture and widely understood basic biosynthetic reactions, these synthetases represent a promising target...
Neutralization of the Positive Charges on Histone Tails by RNA Promotes an Open Chromatin Structure [0.03%]
RNA中和组蛋白尾部正电荷促进染色质开放结构形成
Rositsa Dueva,Karen Akopyan,Chiara Pederiva et al.
Rositsa Dueva et al.
RNA associates extensively with chromatin and can influence its structure; however, the potential role of the negative charges of RNA on chromatin structure remains unknown. Here, we demonstrate that RNA prevents precipitation of histones a...
Customizing Functionalized Cofactor Mimics to Study the Human Pyridoxal 5'-Phosphate-Binding Proteome [0.03%]
功能性辅因子模拟物的定制以研究人类吡哆醛磷酸结合蛋白质组
Anja Fux,Martin Pfanzelt,Volker C Kirsch et al.
Anja Fux et al.
Pyridoxal 5'-phosphate (PLP) is a versatile cofactor that catalyzes a plethora of chemical transformations within a cell. Although many human PLP-dependent enzymes (PLP-DEs) with crucial physiological and pathological roles are known, a glo...
Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions [0.03%]
耐酸可逆切换型绿色荧光蛋白在酸性条件下用于超分辨率成像
Hajime Shinoda,Kai Lu,Ryosuke Nakashima et al.
Hajime Shinoda et al.
Reversibly switchable fluorescent proteins (RSFPs) are crucial tags for super-resolution observation of protein localization and dynamics inside living cells. However, due to the high fluorescence pKa (∼5-6) of most RSFPs, their usage in a...
Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7 [0.03%]
一种细胞通透的强力正向别构调节剂PCP-1染色质调控新小分子化合物CBX7的研究与鉴定
Kelsey N Lamb,Daniel Bsteh,Sarah N Dishman et al.
Kelsey N Lamb et al.
Polycomb-directed repression of gene expression is frequently misregulated in human diseases. A quantitative and target-specific cellular assay was utilized to discover the first potent positive allosteric modulator (PAM) peptidomimetic, UN...
Sourav K Dey,Samie R Jaffrey
Sourav K Dey
Selective RNA degradation is a powerful strategy to combat diseases or study specific RNA function. In this issue of Cell Chemical Biology, Costales et al. (2019) develop a small molecule that mediates selective RNA degradation with the pot...
Sefan Asamitsu,Norifumi Shioda,Hiroshi Sugiyama
Sefan Asamitsu
Aberrant telomerase activity via the expression of its catalytic subunit hTERT is a hallmark of some malignant cancers. In this issue of Cell Chemical Biology, Song et al. (2019) demonstrate that hTERT downregulation by targeting a repressi...
John L Goodier
John L Goodier
In this issue of Cell Chemical Biology, Banuelos-Sanchez et al. (2019) present a comprehensive analysis of selective non-toxic inhibitors of reverse transcriptases encoded by endogenous retrotransposons. This work offers tools for the study...
Targeting the N Terminus of eIF4AI for Inhibition of Its Catalytic Recycling [0.03%]
靶向eIF4AI的N端以抑制其催化再循环
Chenxiao Jiang,Yegen Tang,Lulu Ding et al.
Chenxiao Jiang et al.
DEAD-box ATP-dependent helicases (DEAH/D) are a family of conserved genes predominantly involved in gene expression regulation and RNA processing. As its prototype, eIF4AI is an essential component of the protein translation initiation comp...
Non-covalent NRF2 Activation Confers Greater Cellular Protection than Covalent Activation [0.03%]
非共价型NRF2激活较之共价型激活展现出更优的细胞保护性能
Pengfei Liu,Wang Tian,Shasha Tao et al.
Pengfei Liu et al.
The transcription factor NRF2 confers cellular protection by maintaining cellular redox homeostasis and proteostasis. Basal NRF2 levels are normally low due to KEAP1-mediated ubiquitylation and subsequent proteasomal degradation. KEAP1, a s...