A Crude Extract Preparation and Optimization from a Genomically Engineered Escherichia coli for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline [0.03%]
一种基因工程大肠杆菌粗提物的制备与优化及其在无细胞蛋白质合成系统中的应用-实用操作指南
Jeehye Kim,Caroline E Copeland,Sahana R Padumane et al.
Jeehye Kim et al.
With the advancement of synthetic biology, the cell-free protein synthesis (CFPS) system has been receiving the spotlight as a versatile toolkit for engineering natural and unnatural biological systems. The CFPS system reassembles the mater...
An Effective Protocol for Proteome Analysis of Medaka (Oryzias latipes) after Acute Exposure to Ionizing Radiation [0.03%]
受离子辐射急性暴露后斑马鱼蛋白组分析的有效方案
Yeni Pérez-Gélvez,Shem Unger,Gerardo Gutiérrez-Sánchez et al.
Yeni Pérez-Gélvez et al.
All terrestrial organisms are subject to evolutionary pressures associated with natural sources of ionizing radiation (IR). The legacy of human-induced IR associated with energy, weapons production, medicine, and research has changed the di...
Improving Signal and Photobleaching Characteristics of Temporal Focusing Microscopy with the Increase in Pulse Repetition Rate [0.03%]
通过增加脉冲重复率改善时间聚焦显微镜的信号和光漂白特性
Viktoras Lisicovas,Bala Murali Krishna Mariserla,Chakradhar Sahoo et al.
Viktoras Lisicovas et al.
Wide-field temporal focused (WF-TeFo) two-photon microscopy allows for the simultaneous imaging of a large planar area, with a potential order of magnitude enhancement in the speed of volumetric imaging. To date, low repetition rate laser s...
Solvation Free Energy Simulation for Rosmarinic Acid Extraction from Orthosiphon stamineus [0.03%]
牻牛儿豆玫瑰酸的溶剂化自由能模拟研究
Cher Haan Lau,Lee Suan Chua
Cher Haan Lau
This study was aimed to extract rosmarinic acid from Orthosiphon stamineus Benth. (Lamiaceae) in high yield. The mixture of chloroform-ethyl acetate (70:30) was chosen as the solvent system because rosmarinic acid gave the lowest solvation ...
One-Step ARMS-PCR for the Detection of SNPs-Using the Example of the PADI4 Gene [0.03%]
一步法ARMS－PCR检测单核苷酸多态性及其PADI4基因应用研究
Sabrina Ehnert,Caren Linnemann,Bianca Braun et al.
Sabrina Ehnert et al.
In eukaryotes, cellular functions are tightly controlled by diverse post-translational modifications (PTMs) of proteins. One such PTM affecting many proteins is the deimination of arginine to citrulline. This process, called citrullination ...
Andrea Repele;Manu
Andrea Repele;Manu
Transient Luciferase reporter assays are widely used in the study of gene regulation and intracellular cell signaling. In order to control for sample-to-sample variation in luminescence arising from variability in transfection efficiency an...
Christian Wechselberger,Christian Doppler,David Bernhard
Christian Wechselberger
Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel. F...
Highly Sensitive Shack-Hartmann Wavefront Sensor: Application to Non-Transparent Tissue Mimic Imaging with Adaptive Light-Sheet Fluorescence Microscopy [0.03%]
高灵敏Shack-Hartmann波前传感器在自适应光片荧光显微术非透明组织模拟成像中的应用研究
Javier Morgado Brajones,Gregory Clouvel,Guillaume Dovillaire et al.
Javier Morgado Brajones et al.
High-quality in-depth imaging of three-dimensional samples remains a major challenge in modern microscopy. Selective plane illumination microscopy (SPIM) is a widely used technique that enables imaging of living tissues with subcellular res...
A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases [0.03%]
一种基于紫外/可见光谱法检测核苷和碱基之间相互转化的分析方法
Felix Kaspar,Robert T Giessmann,Niels Krausch et al.
Felix Kaspar et al.
Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation ...
Validity of Ultrasound Imaging Versus Magnetic Resonance Imaging for Measuring Anterior Thigh Muscle, Subcutaneous Fat, and Fascia Thickness [0.03%]
超声波成像与磁共振成像测量大腿前侧肌肉、皮下脂肪和筋膜厚度有效性的对比研究
Filippo Mechelli,Lars Arendt-Nielsen,Maria Stokes et al.
Filippo Mechelli et al.
The aim of the present study was to determine the validity of ultrasound (US) imaging versus magnetic resonance imaging (MRI) for measuring anterior thigh muscle, subcutaneous adipose tissue (SAT), and fascia thickness. Twenty healthy, mode...