Modular reconstruction and multilevel regulation of Saccharomyces cerevisiae to drive the efficient synthesis of asiatic acid [0.03%]
模块化重建和多级调控酿酒酵母高效合成总冠酸的异源途径
Chen Sang,Wenqian Wei,Xinran Yin et al.
Chen Sang et al.
Asiatic acid, a pentacyclic triterpenoid compound, is highly valued in the fields of functional foods, pharmaceuticals, and cosmetics. However, its microbial synthesis has been constrained by an insufficient supply of key precursors. Additi...
Highly biased DNA sequence reconstruction in DNA storage with multi-scale attention mechanism and contrast learning [0.03%]
基于多尺度注意力机制和对比学习的DNA存储中偏置较大的DNA序列重建
Xue Li,Yanfen Zheng,Qi Shao et al.
Xue Li et al.
Base substitution, insertion, and deletion errors due to inherent technical constraints inducing unavoidable sequencing inaccuracies, limiting access to high-quality raw data and biological knowledge. To address this, we propose a deep sequ...
Programmable spatiotemporal control of CRISPR-Cas12a: Engineering precision for next-generation gene editing and diagnostics [0.03%]
可编程的时空CRISPR-Cas12a调控：下一代基因编辑和诊断的精准工程学
Anmol Seelan,Labdhi Shah,Samer Shamshad et al.
Anmol Seelan et al.
CRISPR-Cas12a has emerged as a versatile alternative to Cas9, offering distinct advantages, such as recognition of thymine-rich protospacer adjacent motifs, generation of staggered 5' DNA breaks, simplified guide RNA architecture, and colla...
Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone [0.03%]
工程化植物来源的P450scc从头合成孕烯醇酮
Zikai Chao,Qihang Chen,Wenbao Zhao et al.
Zikai Chao et al.
In contrast to the extensively researched animal CYP11A1 system, the catalytic mechanism of sterol side-chain cleavage by plant-derived cytochrome P450scc enzymes remains poorly understood. Through the integration of computational structura...
High-yield production of hydroxytyrosol through metabolic engineering of Yarrowia lipolytica [0.03%]
里氏木霉代谢工程高效生产羟基酪醇
Bihuan Chen,Yuanyuan Wang,Danlin Wang et al.
Bihuan Chen et al.
Hydroxytyrosol (HT) is a natural antioxidant widely employed in the food production, pharmaceutical, and cosmetic industries. Microbial fermentation for HT production holds significant potential for industrial applications. In this study, w...
P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis [0.03%]
基于结构的理性设计改造CYP P450酶实现高C21选择性及甾体生物合成催化功能
Jian Yang,Rong Li,Qilin Gao et al.
Jian Yang et al.
C21-hydroxylation is a crucial step in the synthesis of corticosteroids. For instance, C21-hydroxylase catalyzes the conversion of progesterone (PRO) into 11-deoxycorticosterone (DOC, the precursor of cortisol and aldosterone). Through stru...
Utilization, metabolic regulation and applications of hydrophobic substrates in Yarrowia lipolytica [0.03%]
用于解脂亚酵母中疏水底物利用、代谢调控及应用的研究进展
Fuzhou Zhu,Lin Zhang,Jianping Wen
Fuzhou Zhu
The efficient use of hydrophobic substrates such as n-alkanes and fatty acids as carbon and energy sources is an important native ability of Yarrowia lipolytica. This review summarizes the crucial characteristics of Y. lipolytica, followed ...
Construction of a library of gene expression elements in industrial Streptomyces and its application [0.03%]
工业产丝状细菌中基因表达元件的构建及应用
Shangyu Li,Daojun Zhu,Liang Wang et al.
Shangyu Li et al.
Streptomycetes are key producers of natural products (NPs), and engineering these organisms represents an effective strategy for enhancing NPs production. However, genetic elements (e.g., promoters and ribosome-binding sites (RBSs)) develop...
Physiological metabolic analysis and process optimization of hypoxia in promoting coenzyme Q10 biosynthesis and accumulation in Rhodobacter sphaeroides HY01 [0.03%]
绿硫细菌HY01中低氧促进辅酶Q_{10}生物合成与积累的生理代谢分析及过程优化
Bo Li,Yan Ge,Li Fu et al.
Bo Li et al.
Coenzyme Q10 fermentation by Rhodobacter sphaeroides (R. sphaeroides) is highly aerobic. However, its biosynthesis and accumulation are paradoxically induced under hypoxic conditions. While oxygen serves as both an induction signal and a ke...
Enzyme promiscuity-driven co-production of flavonoid 7- O-glycosides in engineered Saccharomyces cerevisiae [0.03%]
基于酶促多样性的工程酵母细胞高效合成黄酮类化合物7-O-糖苷簇
Xinjia Tan,Shasha Zuo,Fanglin Hu et al.
Xinjia Tan et al.
Flavonoid 7-O-glycosides are a key class of bioactive flavonoid derivatives with broad application prospects in functional foods and nutraceuticals. Currently, their production is mainly dependent on unsustainable plant extraction or ineffi...