System reconstruction of Bacillus licheniformis for efficient expression of alkaline protease [0.03%]
枯草芽孢杆菌体系重建高效表达碱性蛋白酶工程菌
Qing Zhang,Mengyuan Zhang,Zhihao Zhu et al.
Qing Zhang et al.
Industrial enzymes are widely used in diverse applications, but low productivity limits their further widespread utilization. This research aimed to develop high-performance alkaline protease (AprE) expression strains of Bacillus lichenifor...
An orthogonal transcription system enables high-efficiency rescue of measles virus-based vaccine vectors against multiple pathogens [0.03%]
正交转录系统的存在可高效复活针对多种病原体的麻疹病毒疫苗载体
Zihan Ma,Weijun Wang,Qiuli Lou et al.
Zihan Ma et al.
Recombinant measles virus (rMeV) vectors are promising platforms for vaccine development against emerging infectious diseases due to their safety, stability, and potent immunogenicity. However, conventional rMeV rescue systems frequently ex...
Transfer of chloroplast rbcL and atpB genes to nucleus enables partial rescue of photoautotrophic growth of Chlamydomonas [0.03%]
叶绿体rbcL和atpB基因转移到细胞核内可部分补救衣藻的光自养生长缺陷
Weida Shi,Mengyue Zhu,Chunhui Hou et al.
Weida Shi et al.
An ancient cyanobacterium has evolved through endosymbiosis to form extant chloroplasts of eukaryotic algae and higher plants. During this process, most genes have been transferred to the nuclear genomes. The chloroplast gene sets of differ...
Yongqiang Liu,Jie Hu,Ning Zhang et al.
Yongqiang Liu et al.
With broad-spectrum, low resistance, and multifunctional properties, antimicrobial peptides (AMPs) are promising therapeutic agents against drug-resistant pathogens, yet their discovery and optimization still remain challenging due to the c...
Reassigning an amber codon to selenocysteine using a functional cysteine suppression pair in Escherichia coli [0.03%]
利用大肠杆菌中的半胱氨酸抑制偶联物功能将琥珀密码子重新分配给硒代半胱氨酸
Shaopeng Niu,Jingyan Wei,Mengna Li et al.
Shaopeng Niu et al.
Selenoproteins, characterized by the presence of selenocysteine (Sec) residues, are widely distributed across all domains of life. The unique attributes of selenoproteins are conferred by Sec, which is genetically encoded into nascent polyp...
Tailored biosynthesis of medium chain oleochemicals by engineering fatty acid synthase and β-oxidation [0.03%]
通过工程化脂肪酸合成酶和β-氧化来为中链脂化物的定制生物合成
Simin Cui,Jinming Gao,Xiaobing Yang
Simin Cui
CodY constrains β-cypermethrin degradation by regulating physiological adaptation and soil ecological performance in Bacillus cereus GW-01 [0.03%]
CodY通过调节生理适应和土壤生态性能制约Bacillus cereus GW-01菌株降解高效氯氰菊酯的能力
Ying Lei,Ying Tang,Enting Wei et al.
Ying Lei et al.
β-cypermethrin (β-CY) is a widely used pyrethroid insecticide, yet the regulatory mechanisms that determine microbial degradation efficiency remain unclear. Here, we examined the role of the global nutrient-responsive regulator CodY in β...
N-terminal fusion length: The key to reliable and context-preserving regulatory sequence characterization [0.03%]
氨基端融合长度：可靠且不失真的调控序列特征的关键因素
Sen Yang,Ye Liu,Anqi Li et al.
Sen Yang et al.
Regulatory sequences are commonly characterized using fluorescent reporters, yet how N-terminal coding context shapes these measurements has not been systematically quantified. Here, we evaluated the impact of N-terminal fusion length (45-1...
Characterization of OsNrx3i1, the first intron of rice OsNrx3 gene, as a candidate intron-mediated enhancement element for synthetic promoter engineering [0.03%]
水稻OsNrx3基因的第一个内含子OsNrx3i1是合成启动子工程化的潜在元件
Mengmeng Liu,Chenbo Gong,Jing Zhang et al.
Mengmeng Liu et al.
Intron-mediated enhancement (IME) is a potent endogenous mechanism for boosting gene expression, yet few IME elements function efficiently across distantly related plant species. Here, we characterize the first intron of the rice OsNrx3 gen...
Bingqing Wang,Bo Gao,Quan Wang et al.
Bingqing Wang et al.
PiggyBac (PB) transposons, such as the canonical transposon from Trichoplusia ni and the more recently discovered Mage from Lepidoptera, are "cut-and-paste" mobile genetic elements widely harnessed as non-viral vector systems for gene thera...