Zhongyu Chen,Yelin Duan,Lei Liu et al.
Zhongyu Chen et al.
The discovery of novel scaffold compounds or enzymes through genome mining of biosynthetic gene clusters (BGCs) represents cornerstone of drug development. A major obstacle, however, is that most microbial BGCs encoding secondary metabolite...
Remodeling memory T cells with chemotherapy and immune checkpoint inhibitors as host pre-conditioning to empower in vivo CAR-T therapy [0.03%]
化疗和免疫检查点抑制剂重塑宿主记忆T细胞以增强体内CAR-T治疗疗效
Ding-Ya Sun,Jia-Lu Wang,Xin Li et al.
Ding-Ya Sun et al.
Chemotherapy and immune checkpoint inhibitors (ICIs) are widely utilized in cancer treatment, exerting not only direct cytotoxic effects on tumor cells but also significantly reshaping the systemic immune status of patients, particularly th...
CRISPR/Cas9 mediates precise and efficient gene editing in Xanthomonas oryzae pv. oryzae [0.03%]
CRISPR/Cas9系统在水稻白叶枯病菌中的精准高效基因编辑
Yufeng Luo,Shihao Sun,Jianzhong Jeff Xi
Yufeng Luo
Xanthomonas oryzae pv. oryzae (Xoo), a non-obligate phytopathogen that causes bacterial leaf blight, poses a severe threat to rice production. Although the clustered regularly interspaced short palindromic repeats (CRISPR) system represents...
Xiufang Liu,Mulin Lian,Jiapeng Zeng et al.
Xiufang Liu et al.
Efficient secretion of heterologous proteases in S. cerevisiae remains a major bottleneck due to host cytotoxicity and secretory stress. In this study, we developed a yeast chassis strain through three rounds of iterative evolution, combini...
Constructing an efficient synthesis pathway in peroxisomes to boost β-myrcene production in yeast [0.03%]
Jingyu Chang,Jia Wang,Tianyu Dong et al.
Jingyu Chang et al.
β-Myrcene is a key acyclic monoterpene that has been extensively applied in the agricultural pesticide, fragrance, and flavor industries; it also serves as a critical monoterpene precursor of high-value compounds. Our lab previously succes...
Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla [0.03%]
药用植物云南乌头叶羊齿中四种甾体皂苷生物合成糖基转移酶的的功能研究
Hizar Subthain,Fei Guo,Chengjie Zhang et al.
Hizar Subthain et al.
Paris species are renowned for the production of important medicinal steroidal saponins (e.g., polyphyllins). UDP-dependent glycosyltransferases (UGTs) play important roles in saponin biosynthesis. However, the glycosylation steps have not ...
Shikimate crosstalk enables record-level homogentisic acid production in Yarrowia lipolytica [0.03%]
莽草酸途径交叉启用的裂壶藻中的高水平均二苯酸生产
Chenchen Xu,Wanyue Zhang,Quanlu Zhao et al.
Chenchen Xu et al.
Homogentisic acid (HGA; 2,5-dihydroxyphenylacetic acid) is an aromatic compound with diverse applications in pharmaceuticals, food, and functional materials. Industrial HGA production still relies mainly on multistep chemical synthesis rout...
Modulating ilvA encoding threonine deaminase for balanced growth and PHB synthesis by Halomonas grown in rich nitrogen source [0.03%]
在丰富的氮源中调控编码苏氨酸脱氨酶的ilvA基因以平衡生长和聚羟基丁酸合成能力
Junting Sheng,Yuying Guan,Jiale Wang et al.
Junting Sheng et al.
Bioplastic poly(3-hydroxybutyrate) (PHB) production by Halomonas bluephagenesis is typically activated under nitrogen limitation, inevitably restricting biomass formation and overall productivity. Here we identified gene ilvA encoding threo...
Discovery of functional compounds from dimorphic edible Ophiocordyceps sinensis fungus in host haemolymph [0.03%]
冬虫夏草菌分生期次级代谢产物研究发现功能化合物
Tao Sun,Yutong Yang,Jie Xie et al.
Tao Sun et al.
Chinese cordyceps, with a fruiting body of Ophiocordyceps sinensis from insect cadaver, is well-known health food. During formation, O. sinensis experiences a dimorphic transition from blastospores to hyphae in Thitarodes host haemolymoph. ...
Systematic metabolic engineering of Escherichia coli for high-level production of pseudouridine via pathway optimization and precursor enhancement [0.03%]
通过途径优化和前体增强系统性地对大肠杆菌进行代谢工程改造高效生产假尿苷
Jing Song,Wei Shen,Yuanyuan Xia et al.
Jing Song et al.
Pseudouridine, the C5-ribose epimer of uridine with significant biological functions and clinical applications, was efficiently produced through systematic metabolic strategy of Escherichia coli in this study. Initial overexpression of pseu...