In-silico identification of phytochemical compounds from various medicinal plants as potent HIV-1 non-nucleoside reverse transcriptase inhibitors utilizing molecular docking and molecular dynamics simulations [0.03%]
基于分子对接和分子动力学模拟的植物化学成分抗HIV-1非核苷类逆转录酶抑制剂的体外筛选及机制研究
Suleiman Danladi,Ayinde Abdulwahab Adeniyi,Zainab Iman Sani et al.
Suleiman Danladi et al.
HIV is a global public health challenge. The Reverse Transcriptase (RT) enzyme facilitates an important step in HIV replication. Inhibition of this enzyme provides a critical target for HIV treatment. The aim of this study is to employ comp...
NEAT and tidy: a novel RT-PCR method for the independent quantification of the NEAT1_1 isoform [0.03%]
简洁而整洁：一种独立测定NEAT1 _ 1亚型的新型RT-qPCR方法
Nadine E Smith,Janni Petersen,Michael Z Michael
Nadine E Smith
The long noncoding RNA NEAT1 is transcribed from a single exon gene and produces two isoforms through alternative 3'-end processing. The short polyadenylated NEAT1_1 drives proliferation in many malignancies through increasing glycolytic fl...
ThermoPlex: an automated design tool for target-specific multiplex PCR primers based on DNA thermodynamics [0.03%]
基于DNA热力学的靶标特异性多重PCR引物自动化设计工具ThermoPlex
Altair Agmata,Kevin Labrador,Joseph Dominic Palermo et al.
Altair Agmata et al.
Multiplex PCR-based assays are indispensable platforms for rapid and cost-effective DNA-based multi-target detection. The success of such an assay highly depends on the accurate design of oligonucleotide primers, arguably its most vital com...
Comparative analysis of paraffin and JB-4 embedding techniques in light microscopy [0.03%]
石蜡包埋和JB-4包埋技术的光镜比较观察
Zeynep Deniz Şahin İnan,Rasim Hamutoğlu,Serpil Ünver Saraydın
Zeynep Deniz Şahin İnan
Histological embedding and staining techniques are essential for examining tissue and cellular morphology. This study compares two embedding methods-JB-4™, a glycol methacrylate-based resin, and conventional paraffin-to determine which met...
Correction to: AllerTrans: a deep learning method for predicting the allergenicity of protein sequences [0.03%]
过敏原预测工具AllerTrans的补充修正文章
[This corrects the article DOI: 10.1093/biomethods/bpaf040.]. © The Author(s) 2025. Published by Oxford University Press.
Published Erratum
Biology methods & protocols. 2025 Nov 8;10(1):bpaf076. DOI:10.1093/biomethods/bpaf076 2025
Enhanced protocol for measuring glutathione peroxidase activity using a new glutathione peroxidase-Tiron assay [0.03%]
一种改进的谷胱甘肽过氧化物酶活性测定方法及其新试剂盒的应用研究
Mahmoud Hussein Hadwan,Abbas Ali Mohammed,Saeed Najavand et al.
Mahmoud Hussein Hadwan et al.
Conventional methods for measuring glutathione peroxidase (GPx) activity are limited by interference issues, complex protein precipitation steps, and variable reliability, necessitating the development of improved analytical approaches for ...
Amplicon sequence proportion: A novel method for HRM primer design in DNA methylation analysis among marginalized rural population in Southern Mexico [0.03%]
靶向扩增子序列比例在墨西哥南部边缘化农村人群DNA甲基化分析中高分辨率熔解曲线甲基化特异性PCR设计中的应用研究
Christian Medina-Gómez,Pilar Elena Núñez-Ortega,Itandehui Castro-Quezada et al.
Christian Medina-Gómez et al.
DNA methylation is an important modification in the genomes, participating in gene expression or gene repression, as a part of epigenetic studies. This modification can be studied with last-generation sequencing or using PCR coupled with Hi...
A novel C. elegans respirometry assay using low-cost optical oxygen sensors [0.03%]
一种使用低成本光学氧传感器的新型秀丽隐杆线虫呼吸分析方法
Nathan Dennis,Campbell W Gourlay,Marina Ezcurra
Nathan Dennis
Measurement of the oxygen consumption rate, or respirometry, is a powerful and comprehensive method for assessing mitochondrial function both in vitro and in vivo. Respirometry at the whole-organism level has been repeatedly performed in th...
Xiaoyu Duan,Vipul Periwal
Xiaoyu Duan
Inferring nonlinear dynamics and parameters in biological data modeling is challenging. Standard parameter optimization methods are difficult to constrain to biological ranges, especially for nonlinear models. We propose a novel method to e...
Modeling internal charge effects on capacitor dynamics for non-invasive estimation of membrane potential [0.03%]
模型化内部电荷对电容动力学的影响以非侵入方式估算膜电位
Xiaofeng Ma
Xiaofeng Ma
Accurate and non-invasive measurement of cell membrane potential is essential for studying physiological processes and disease mechanisms. In this study, we propose a conceptual and computational model for estimating membrane potential base...