Transmission electron microscopy of cultured cells: Optimized protocols for ultrastructural studies and post-embedding immunogold labeling [0.03%]
透射电镜观察培养细胞：亚细胞结构的研究及免疫电镜的优化方法
Anna Richert,Robert Lenartowski,Jolanta Nowak et al.
Anna Richert et al.
Nanometer-scale localization of specific proteins within cells is essential for understanding their roles in diverse physiological and pathological cellular processes. Such high-resolution localization of protein epitopes can be achieved by...
Cannabinoid GPCRs, ectopic olfactory GPCRs and TRP channels: a prespecified baseline-edit-rescue framework for testing higher-order membrane-conditioned integration [0.03%]
cannabinoid G 蛋白偶联受体、异位嗅觉G蛋白偶联受体和瞬态感受器电位通道：用于测试更高阶的膜条件整合的预设基线编辑救援框架
Erhan Yarar
Erhan Yarar
Cell membranes are not mere platforms for signalling proteins; they can shape how receptor inputs are assembled into local responses. In membrane-rich microdomains, receptor identification and pathway mapping do not reveal the logic of a me...
Gap junction communication regulates luminal-myoepithelial crosstalk and cell differentiation in a bilayered human mammary epithelial cell model [0.03%]
缝隙连接通讯调节双层人乳腺上皮细胞模型中腔上皮-肌上皮的相互作用及细胞分化
Alec McDermott,Melany Nicole Juarez,David Tovar Parra et al.
Alec McDermott et al.
Interactions between luminal and myoepithelial cells are essential for proper mammary gland development and function. However, only a few in vitro models allow the study of these interactions and their modulation by extrinsic factors. We de...
Nonlinear combinatorial analysis of blood transcriptomes identifies PRKAR1A as a regulator of TDP-43 pathophysiology in amyotrophic lateral sclerosis [0.03%]
非线性组合分析血浆转录组鉴定出PRKAR1A为运动神经元疾病中TDP-43病理生理学的调节因子
Keiko Imamura,Ayako Nagahashi,Aya Okusa et al.
Keiko Imamura et al.
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive loss of motor neurons. Accurate and accessible blood-based diagnostics for neurodegenerative diseases, including ALS, are being progressiv...
Assessing the feasibility of machine learning for ancient DNA age prediction: Limitations and insights [0.03%]
评估机器学习在古代DNA年龄预测中的可行性：限制与见解
Maksim Kazanskii,Maria Golikova,Artem Kasianov et al.
Maksim Kazanskii et al.
We investigated the possibility of estimating the age of ancient biological samples directly from their DNA damage profiles using supervised machine learning. Traditional dating methods such as radiocarbon dating or dendrochronology rely on...
Advances in the detection of antimicrobial resistance in aquatic environments: a methodological perspective [0.03%]
水环境中抗生素抗性检测研究进展：方法学视角
Olukayode Adebola Ibitoye,Chinyere Nkemjika Anyanwu,Abdulganiy Babatunde Agbaje et al.
Olukayode Adebola Ibitoye et al.
Antimicrobial resistance (AMR) is a global health and environmental challenge, driven by complex interactions among microbial communities, resistance genes, and selective pressures in various ecological niches. Traditional surveillance proc...
Xue Sun,Oren Ram
Xue Sun
This protocol describes sc‑rDSeq, a scalable, droplet‑based method for full‑length, strand‑specific total RNA sequencing at single‑cell resolution. The protocol uses a refined set of 220 ribosomal‑depleted sequences (rDS) primers that...
Discovery of novel InhA inhibitors through structural bioinformatics and machine learning-driven QSAR screening of natural products [0.03%]
基于结构的生物信息学和机器学习驱动的QSAR筛选发现抗结核药物InhA的新抑制剂
Godswill Imolele Anthony,Samuel Nzube Nwosu,Adedoyin John-Joy Owolade et al.
Godswill Imolele Anthony et al.
Tuberculosis (TB) persists as a formidable global health challenge, particularly due to the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) variants that limit the effectiveness of existing therapies. These varia...
Correction [0.03%]
改正通知书
[This corrects the article DOI: 10.1093/biomethods/bpaf077.]. © The Author(s) 2026. Published by Oxford University Press.
Published Erratum
Biology methods & protocols. 2026 Jun 16;11(1):bpag030. DOI:10.1093/biomethods/bpag030 2026
Viral Sentry AI-Automated zoonotic surveillance and drug repurposing agent [0.03%]
AI自动化兽媒监视和药物再利用机器人
Cristian R Munteanu,Jose Vázquez-Naya,Eduardo Tejera
Cristian R Munteanu
Zoonotic viruses capable of jumping from animal reservoirs into human populations represent a persistent and unpredictable menace to global health. To confront this challenge, we developed Viral Sentry AI, an autonomous agent designed to cl...