Dewey Brooke,Navid Movahed,Brian Bothner
Dewey Brooke
The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins. Buffer molecules can induce changes in confo...
Imaging flow cytometry methods for quantitative analysis of label-free crystalline silica particle interactions with immune cells [0.03%]
用于定量分析无标签结晶二氧化硅颗粒与免疫细胞相互作用的成像流式细胞术方法
Bradley Vis,Jonathan J Powell,Rachel E Hewitt
Bradley Vis
Exposure to respirable fractions of crystalline silica quartz dust particles is associated with silicosis, cancer and the development of autoimmune conditions. Early cellular interactions are not well understood, partly due to a lack of sui...
What is the role of histone H1 heterogeneity? A functional model emerges from a 50 year mystery [0.03%]
组蛋白H1异质性的作用是什么？一个功能模型从50年的谜团中浮现出来
Missag Hagop Parseghian
Missag Hagop Parseghian
For the past 50 years, understanding the function of histone H1 heterogeneity has been mired in confusion and contradiction. Part of the reason for this is the lack of a working model that tries to explain the large body of data that has be...
Samantha B Gacasan,Daniel L Baker,Abby L Parrill
Samantha B Gacasan
G protein-coupled receptors (GPCR) comprise a diverse superfamily of over 800 proteins that have gained relevance as biological targets for pharmaceutical drug design. Although these receptors have been investigated for decades, three-dimen...
Single-molecule FRET reveals proofreading complexes in the large fragment of Bacillus stearothermophilus DNA polymerase I [0.03%]
单分子FRET揭示了嗜热芽孢杆菌DNA聚合酶I大片段中的校对复合物
Thomas V Christian,William H Konigsberg
Thomas V Christian
There is increasing interest in the use of DNA polymerases (DNA pols) in next-generation sequencing strategies. These methodologies typically rely on members of the A and B family of DNA polymerases that are classified as high-fidelity DNA ...
The effect of different divalent cations on the kinetics and fidelity of Bacillus stearothermophilus DNA polymerase [0.03%]
不同二价阳离子对嗜热脂肪芽胞杆菌DNA聚合酶的动力学及保真性的影响
Ashwani Kumar Vashishtha,William H Konigsberg
Ashwani Kumar Vashishtha
Although Mg2+ is the metal ion that functions as the cofactor for DNA polymerases (DNA pols) in vivo, Mn2+ can also serve in this capacity but it reduces base discrimination. Metal ions aside from Mg2+ or Mn2+ can act as cofactors for some ...
Free-radicals and advanced chemistries involved in cell membrane organization influence oxygen diffusion and pathology treatment [0.03%]
自由基和先进化学在细胞膜组织中的作用影响氧气扩散及病理学治疗
Richard C Petersen
Richard C Petersen
A breakthrough has been discovered in pathology chemistry related to increasing molecular structure that can interfere with oxygen diffusion through cell membranes. Free radicals can crosslink unsaturated low-viscosity fatty acid oils by ch...
Z Hong Zhou,Joshua Chiou
Z Hong Zhou
First discovered in bacteriophage HK97, biological chainmail is a highly stable system formed by concatenated protein rings. Each subunit of the ring contains the HK97-like fold, which is characterized by its submarine-like shape with a 5-s...
Joshua Holcomb,Nicholas Spellmon,Yingxue Zhang et al.
Joshua Holcomb et al.
To date, X-ray crystallography remains the gold standard for the determination of macromolecular structure and protein substrate interactions. However, the unpredictability of obtaining a protein crystal remains the limiting factor and cont...
Mechanisms and applications of the anti-inflammatory effects of photobiomodulation [0.03%]
光生物调节抗炎作用的机制与应用
Michael R Hamblin
Michael R Hamblin
Photobiomodulation (PBM) also known as low-level level laser therapy is the use of red and near-infrared light to stimulate healing, relieve pain, and reduce inflammation. The primary chromophores have been identified as cytochrome c oxidas...