Validation of an automated total and viable cell counting for GMP-compliant production of human bone marrow stromal cells [0.03%]
人骨髓stromal细胞GMP生产自动总细胞和活细胞计数法验证
Scarlett Desclaux,Narong Chitchongyingcharoen,Chanatat Threeratanah et al.
Scarlett Desclaux et al.
Bone marrow stromal cells (BMSCs) are classified as Advanced Therapy Medicinal Products (ATMPs) in the subcategory of Cell Therapy Medicinal Products (CTMPs), and the production of these cells for clinical use under good manufacturing pract...
Domain shuffling of endolysin LysKB317 to control Limosilactobacillus fermentum in corn mash fermentation [0.03%]
域转移内溶酶LysKB317调控玉米浆发酵中乳酸片球菌的生长
Shao-Yeh Lu,Zhengliang L Wu,Moses Y Martinez et al.
Shao-Yeh Lu et al.
Lactic acid bacteria (LAB), such as Limosilactobacillus fermentum are persistent contaminants in bioethanol fermentation facilities and inhibit Saccharomyces cerevisiae, reducing ethanol yields. Rising resistance to antibiotics such as virg...
Performance comparison of downstream unit operations and process chains for the purification of amino acids for food, feed and pharma applications [0.03%]
食品、饲料和制药应用氨基酸纯化下游单元操作及工艺流程的比较研究
Maximilián Prachár,J F Buyel
Maximilián Prachár
Proteinogenic amino acids are important supplements in foods, feeds, and pharmaceuticals. However, the development of cost-efficient and scalable platform downstream processes for multi-ton manufacturing of amino acids is complicated by ups...
Biochemical and functional benchmarking of plant protein hydrolysates in serum-reduced adherent CHO-K1 cell culture [0.03%]
无血清条件下植物源蛋白酶解物在CHO-K1细胞培养中作为补充剂的生化及功能特性评估
F Compeau,G Yalkin,P Mortamais et al.
F Compeau et al.
Chinese Hamster Ovary (CHO) cells are used for therapeutic protein production. Although industrial platforms rely on serum-free media, fetal bovine serum (FBS) remains common in research and development. Reducing serum while maintaining cel...
Green synthesis of silver nanoparticles under ambient conditions using onion, turmeric, and galangal extracts with antibacterial activity against E. coli and S. aureus [0.03%]
常温下用洋葱、姜黄和高良姜提取物绿色合成银纳米颗粒并对其对抗大肠杆菌和金黄色葡萄球菌的抗菌活性的研究
Arkarapol Thumwong,Pimsiree Suwanna,Ekachai Wimolmala et al.
Arkarapol Thumwong et al.
This study developed a sustainable method for synthesizing antibacterial silver nanoparticles (AgNPs) using aqueous extracts of onion, galangal, and turmeric as natural reducing and stabilizing agents under ambient conditions. The extracts ...
Adaptive responses of Chlamydomonas reinhardtii to cadmium stress: physiological, biochemical, and lipidomic insights [0.03%]
绿藻门模式生物小立皱臂鞭藻对镉胁迫的适应性响应：生理生化及脂质组学研究视角
Mohammad Aadil,Nihad Sahri,Mutale Joan Chanda et al.
Mohammad Aadil et al.
Cadmium (Cd), a toxic heavy metal, poses a significant threat to aquatic ecosystems by impairing primary producers such as microalgae. This study investigated the adaptive responses of Chlamydomonas reinhardtii to Cd stress through physiolo...
High-throughput mass spectrometry for screening microbial culture supernatants for aromatic amino acids and selected derivatives [0.03%]
高效质谱筛选微生物培养上清中的芳香族氨基酸及特定衍生物
Amisha Patel,Stefan Stagge,Payam Ghiaci et al.
Amisha Patel et al.
Sustainable production of aromatic amino acids (AAAs) and their derivatives are central to developing renewable routes for aroma- and flavour-related chemicals with myriads of applications in pharmaceutical industry, food and nutrition, cos...
Advances in electrochemical immunosensors for tuberculosis detection: a systematic review [0.03%]
用于结核病检测的电化学免疫传感器研究进展——系统综述
Sasan Radfar,Mostafa Teymuri,Michael Holzinger et al.
Sasan Radfar et al.
Tuberculosis (TB) remains a major global health challenge, emphasizing the need for rapid and reliable diagnostic tools. As technologies move toward simplicity, portability, and real-time analysis, electrochemical biosensors have emerged as...
Dissection of MAPK and NF-κB p65 signaling dynamics underlying LPS tolerance in macrophages at the single-cell level [0.03%]
单细胞水平下脂多糖耐受巨噬细胞中MAPK和NF-κB P65信号动力学的解析研究
Tuntikorn Laosuk,Patipark Kueanjinda,Hiroshi Kimura et al.
Tuntikorn Laosuk et al.
MAPK and NF-κB pathways regulate macrophage responses to microbial stimuli. Repeated exposure to lipopolysaccharide (LPS) induces endotoxin tolerance, a state in which inflammatory cytokine production is suppressed while antimicrobial func...
Silver nanoparticles enhance organogenesis, total flavonoid and anthocyanin contents in Gynura pseudochina tissue culture [0.03%]
银纳米颗粒增强Gynura pseudochina组织培养的器官发生、总黄酮和花青素含量
Nurul Jadid,Maulidia Rahmawati,Aiziah Ayu Pratami et al.
Nurul Jadid et al.
Gynura pseudochina is an Indonesian medicinal herb valued for its flavonoid and anthocyanin content. Despite its pharmacological importance, approaches to enhance metabolite accumulation and clarify associated molecular responses remain lim...