Jonathan Asmund Arnesen,Irina Borodina
Jonathan Asmund Arnesen
Terpenoids are a group of chemicals of great importance for human health and prosperity. Terpenoids can be used for human and animal nutrition, treating diseases, enhancing agricultural output, biofuels, fragrances, cosmetics, and flavourin...
Isobutanol production by combined in vivo and in vitro metabolic engineering [0.03%]
体内与体外相结合的代谢工程联用技术用于异丁醇生产
Mamta Gupta,Matthew Wong,Kamran Jawed et al.
Mamta Gupta et al.
The production of the biofuel, isobutanol, in E. coli faces limitations due to alcohol toxicity, product inhibition, product recovery, and long-term industrial feasibility. Here we demonstrate an approach of combining both in vivo with in v...
Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering [0.03%]
基于微生物代谢工程的木质纤维素半纤维素高值生物制品合成
Biao Geng,Xiaojing Jia,Xiaowei Peng et al.
Biao Geng et al.
Hemicellulose is the second most abundant carbohydrate in lignocellulosic biomass and has extensive applications. In conventional biomass refinery, hemicellulose is easily converted to unwanted by-products in pretreatment and therefore can'...
Metabolomics and modelling approaches for systems metabolic engineering [0.03%]
代谢组学和建模方法在系统代谢工程中的应用
Jasmeet Kaur Khanijou,Hanna Kulyk,Cécilia Bergès et al.
Jasmeet Kaur Khanijou et al.
Metabolic engineering involves the manipulation of microbes to produce desirable compounds through genetic engineering or synthetic biology approaches. Metabolomics involves the quantitation of intracellular and extracellular metabolites, w...
RNA polymerase II-driven CRISPR-Cas9 system for efficient non-growth-biased metabolic engineering of Kluyveromyces marxianus [0.03%]
用于高效克劳维氏毕赤酵母代谢工程的RNA聚合酶II驱动的CRISPR-Cas9系统
Danielle Bever,Ian Wheeldon,Nancy Da Silva
Danielle Bever
The thermotolerant yeast Kluyveromyces marxianus has gained significant attention in recent years as a promising microbial candidate for industrial biomanufacturing. Despite several contributions to the expanding molecular toolbox for gene ...
Chien-Yuan Lin,Yang Tian,Kimberly Nelson-Vasilchik et al.
Chien-Yuan Lin et al.
Engineering bioenergy crops to accumulate coproducts in planta can increase the value of lignocellulosic biomass and enable a sustainable bioeconomy. In this study, we engineered sorghum with a bacterial gene encoding a chorismate pyruvate-...
Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production [0.03%]
调优一个多路复用的CRISPR干扰型Pseudomonas putida菌株以进一步增强天青素的生产
Jeffrey J Czajka,Deepanwita Banerjee,Thomas Eng et al.
Jeffrey J Czajka et al.
In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous indigoidine production was examined using three distinct omic datasets. Transcriptomic data indicated that CRISPR/dCpf1-interference (CRISPRi) mediated multip...
A targeted metabolomics method for extra- and intracellular metabolite quantification covering the complete monolignol and lignan synthesis pathway [0.03%]
一种针对单木脂素和木脂素合成途径全面覆盖的胞内和胞外代谢物定量方法
Andrea Steinmann,Katrin Schullehner,Anna Kohl et al.
Andrea Steinmann et al.
Microbial synthesis of monolignols and lignans from simple substrates is a promising alternative to plant extraction. Bottlenecks and byproduct formation during heterologous production require targeted metabolomics tools for pathway optimiz...
Dynamic and single cell characterization of a CRISPR-interference toolset in Pseudomonas putida KT2440 for β-ketoadipate production from p-coumarate [0.03%]
用于从对羟基苯甲酸生产β-酮己二酸的大肠杆菌CRISPR干扰工具集的单细胞表征与动力学研究
Jacob A Fenster,Allison Z Werner,Jian Wei Tay et al.
Jacob A Fenster et al.
Pseudomonas putida KT2440 is a well-studied bacterium for the conversion of lignin-derived aromatic compounds to bioproducts. The development of advanced genetic tools in P. putida has reduced the turnaround time for hypothesis testing and ...
Kyle R Pomraning,Ziyu Dai,Nathalie Munoz et al.
Kyle R Pomraning et al.
The global regulator LaeA controls secondary metabolism in diverse Aspergillus species. Here we explored its role in regulation of itaconic acid production in Aspergillus pseudoterreus. To understand its role in regulating metabolism, we de...