Engineering of the glucose uptake system to increase 2,4-Dihydroxybutyric acid production in Escherichia coli [0.03%]
工程化葡萄糖摄取系统以提高大肠杆菌中2,4-二羟基丁酸的产量
T A Stefanie Nguyen,Ceren Alkim,Nadine Ihle et al.
T A Stefanie Nguyen et al.
2,4-Dihydroxybutyric acid (DHB) is a promising C4 platform compound for the synthesis of methionine analogues and biodegradable polymers. However, aerobic DHB production from glucose in Escherichia coli involves transient acetate overflow p...
A systems-level view of metabolic impact of copper and methane valorization potential in Methylosinus trichosporium OB3b [0.03%]
甲基球菌OB3b中铜和甲烷的代谢作用的系统研究及潜在价值
Junwon Yang,Jeremy D Semrau
Junwon Yang
Methanotrophs, which utilize methane as their sole carbon and energy source, are promising platforms for valorizing methane to industrially valuable products, such as bioplastics or biofuels. However, methanotrophic activity is highly affec...
Metabolic engineering of Methanothermobacter thermautotrophicus ΔH for recombinant acetoin production [0.03%]
用于重组生产丙酮酸的Methanothermobacter thermautotrophicus ΔH代谢工程
Tina Baur,Maximilliene T Allaart,Aaron Zipperle et al.
Tina Baur et al.
Thermophilic methanogens of the genus Methanothermobacter are established biocatalysts in power-to-gas applications, converting H2 and CO2 into CH4 through the process of methanogenesis. Further expanding this platform for the bioproduction...
Multi-layered metabolic remodeling of Pseudomonas putida for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel [0.03%]
芽孢杆菌的多层代谢重构高效转化为先进航空燃料前体化合物
Chae Won Kang,David N Carruthers,Joshua McCauley et al.
Chae Won Kang et al.
Isoprenyl acetate, a volatile ester derived from isoprenol, is a key biosynthetic intermediate for the advanced aviation fuel candidate, 1,4-dimethylcyclooctane. Here, we engineered Pseudomonas putida KT2440 for the production of isoprenyl ...
Engineering Pseudomonas taiwanensis for efficient chorismate-based production of mono- and dihydroxybenzoates [0.03%]
工程改造天台土壤假单胞菌以高效合成香豆酸及邻羟苯甲酸类化合物
Franziska Kofler,Tobias Schwanemann,Nadine Teófilo da Silva et al.
Franziska Kofler et al.
Aromatics have many important applications in modern society but are traditionally produced in non-sustainable processes from fossil resources. Whole-cell biocatalysis bears great potential to provide a variety of aromatics from renewable c...
Fine modulation of carbon flow in central carbon metabolism via ribosome-binding site modification in Escherichia coli [0.03%]
通过调节启动子强度和核糖体结合位点精细调控大肠杆菌中心碳代谢流量
Shogo Sawada,Tatsumi Imada,Hikaru Nagai et al.
Shogo Sawada et al.
Optimization of flux distribution in central carbon metabolism is important to improve the microbial productivity. As the number of precursors required for synthesis differs for each target compound, optimal flux distribution also varies. A...
Elucidating biodegradation of dimethyl terephthalate by two Rhodococcus strains for its valorization applications [0.03%]
两种罗德氏菌株降解dimethyl terephthalate（DMT）的研究及其高值化应用初探
Yifeng Hu,Tae Seok Moon
Yifeng Hu
Dimethyl terephthalate (DMT) serves as the precursor in the production of polyethylene terephthalate, polytrimethylene terephthalate, and polybutylene terephthalate. The widespread use of DMT in the polymer industry and its ubiquitous exist...
Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose [0.03%]
以纤维素为原料生产2,3-丁二醇的解糖梭菌工程菌的构建
S Bilal Jilani,Nandhini Ashok,Yannick J Bomble et al.
S Bilal Jilani et al.
Clostridium thermocellum is a promising host for consolidated bioprocessing due to its ability to directly ferment cellulose into fuels and chemicals. However, natural product formation in this organism is limited. Here, we report engineeri...
Mechanistic and applied study of phosphofructokinases, the "gatekeeper" of the glycolytic pathway on the central carbon metabolism [0.03%]
果糖磷酸激酶的机制及应用研究：作为中央碳代谢途径中的糖酵解“守门员”
Lingyun Li,Xin Chen,Yijie Zhang et al.
Lingyun Li et al.
Phosphofructokinase (Pfk), a key regulatory enzyme in glycolysis, is composed of Pfk1 and Pfk2 subunits in Saccharomyces cerevisiae. However, the distinct roles of these subunits in central carbon metabolism remain unclear. Here, we examine...
Model-guided chemical environment and metabolic network design to couple pathways with cell fitness [0.03%]
基于模型的化学环境和代谢网络设计，用于连接途径与细胞适应度
Natalia Kakko von Koch,Tuula Tenkanen,Sandra Castillo et al.
Natalia Kakko von Koch et al.
Heterologous compound production is a complex trait since the native metabolic fluxes supplying the precursors, redox power, and energy are under multilevel cellular regulation. Improving complex traits using targeted engineering needs comb...