Tuning a high performing multiplexed-CRISPRi Pseudomonas putida strain to further enhance indigoidine production
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In this study, a 14-gene edited Pseudomonas putida KT2440 strain for heterologous indigoidine production was examined using three distinct omic datasets. Transcriptomic data indicated that CRISPR/dCpf1-interference (CRISPRi) mediated multiplex repression cause... ...