Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds [0.03%]
利用木质素衍生物生产牻牛儿acetate的拜氏不动杆菌ADP1工程菌株
Erika Arvay,Bradley W Biggs,Laura Guerrero et al.
Erika Arvay et al.
Utilization of lignin, an abundant renewable resource, is limited by its heterogenous composition and complex structure. Biological valorization of lignin provides advantages over traditional chemical processing as it occurs at ambient temp...
Identification of a lichen depside polyketide synthase gene by heterologous expression in Saccharomyces cerevisiae [0.03%]
异源表达筛选到的地衣多步合酶基因 deputies
James T Kealey,James P Craig,Philip J Barr
James T Kealey
Lichen-forming fungi produce a variety of secondary metabolites including bioactive polyketides. Advances in DNA and RNA sequencing have led to a growing database of new lichen gene clusters encoding polyketide synthases (PKS) and associate...
Cell line development for continuous high cell density biomanufacturing: Exploiting hypoxia for improved productivity [0.03%]
连续高细胞密度生物制造的细胞系开发：利用低氧提高生产力
Nikolas Zeh,Patrick Schlossbauer,Nadja Raab et al.
Nikolas Zeh et al.
Oxygen deficiency (hypoxia) induces adverse effects during biotherapeutic protein production leading to reduced productivity and cell growth. Hypoxic conditions occur during classical batch fermentations using high cell densities or perfusi...
Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants [0.03%]
通过重新定向烟草植物代谢流向香豆酸途径来产生 raspberries酮酸
Takao Koeduka,Sachiho Takarada,Koya Fujii et al.
Takao Koeduka et al.
Raspberry ketone is one of the characteristic flavors of raspberry fruits, and it is an important and expensive ingredient in the flavor and fragrance industries. It is present at low levels in plant tissues, and its occurrence is limited t...
A proline metabolism selection system and its application to the engineering of lipid biosynthesis in Chinese hamster ovary cells [0.03%]
一种脯氨酸代谢筛选系统及其在工程改造CHO细胞脂质生物合成中的应用
James D Budge,Joanne Roobol,Gurdeep Singh et al.
James D Budge et al.
Chinese hamster ovary (CHO) cells are the leading mammalian cell host employed to produce complex secreted recombinant biotherapeutics such as monoclonal antibodies (mAbs). Metabolic selection marker technologies (e.g. glutamine synthetase ...
mfapy: An open-source Python package for 13C-based metabolic flux analysis [0.03%]
MFAPA：基于13C的代谢流分析的开源Python软件包
Fumio Matsuda,Kousuke Maeda,Takeo Taniguchi et al.
Fumio Matsuda et al.
13C-based metabolic flux analysis (13C-MFA) is an essential tool for estimating intracellular metabolic flux levels in metabolic engineering and biology. In 13C-MFA, a metabolic flux distribution that explains the observed isotope labeling ...
Electronic signals are electrogenetically relayed to control cell growth and co-culture composition [0.03%]
电子信号通过电遗传学方式进行传导以控制细胞生长和共培养组成比
Kristina Stephens,Fauziah Rahma Zakaria,Eric VanArsdale et al.
Kristina Stephens et al.
There is much to be gained by enabling electronic interrogation and control of biological function. While the benefits of bioelectronics that rely on potential-driven ionic flows are well known (electrocardiograms, defibrillators, neural pr...
Anton Puzorjov,Katherine E Dunn,Alistair J McCormick
Anton Puzorjov
Phycocyanin (PC) is a soluble phycobiliprotein found within the light-harvesting phycobilisome complex of cyanobacteria and red algae, and is considered a high-value product due to its brilliant blue colour and fluorescent properties. Howev...
Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae [0.03%]
代谢工程酵母由木糖和葡萄糖高效生产3-羟基丙酸
Kanchana R Kildegaard,Zheng Wang,Yun Chen et al.
Kanchana R Kildegaard et al.
Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can co...
Redistribution of metabolic fluxes in Chlorella protothecoides by variation of media nitrogen concentration [0.03%]
通过改变培养基氮浓度调节小球藻代谢路径分布
Saratram Gopalakrishnan,Jordan Baker,Linda Kristoffersen et al.
Saratram Gopalakrishnan et al.
In this study, the Elementary Metabolite Unit (EMU) algorithm was employed to calculate intracellular fluxes for Chlorella protothecoides using previously generated growth and mass spec data. While the flux through glycolysis remained relat...