Generation of highly pure pluripotent stem cell-derived myogenic progenitor cells and myotubes [0.03%]
生成高度纯化的多能干细胞来源的肌源性祖细胞和肌管
Reem Bou Akar,Chéryane Lama,Déborah Aubin et al.
Reem Bou Akar et al.
Driving efficient and pure skeletal muscle cell differentiation from pluripotent stem cells (PSCs) has been challenging. Here, we report an optimized protocol that generates skeletal muscle progenitor cells with high efficiency and purity i...
Human retinal organoids with an OPA1 mutation are defective in retinal ganglion cell differentiation and function [0.03%]
携带OPA1突变的人视网膜类器官中视神经节细胞的分化和功能异常
Qiannan Lei,Kangjian Xiang,Lin Cheng et al.
Qiannan Lei et al.
Autosomal dominant optic atrophy (ADOA), mostly caused by heterozygous OPA1 mutations and characterized by retinal ganglion cell (RGC) loss and optic nerve degeneration, is one of the most common types of inherited optic neuropathies. Previ...
A critical role of retinoic acid concentration for the induction of a fully human-like atrial action potential phenotype in hiPSC-CM [0.03%]
全人源性心房动作电位诱导的关键视黄酸浓度
Carl Schulz,Muhammed Sönmez,Julia Krause et al.
Carl Schulz et al.
Published Erratum
Stem cell reports. 2023 Dec 12;18(12):2528. DOI:10.1016/j.stemcr.2023.11.001 2023
Eyes open on stem cells [0.03%]
干细胞研究光明在望
Anna Altshuler,Aya Amitai-Lange,Waseem Nasser et al.
Anna Altshuler et al.
Recently, the murine cornea has reemerged as a robust stem cell (SC) model, allowing individual SC tracing in living animals. The cornea has pioneered seminal discoveries in SC biology and regenerative medicine, from the first corneal trans...
Juliette Wohlschlegel,Connor Finkbeiner,Dawn Hoffer et al.
Juliette Wohlschlegel et al.
In mammals, loss of retinal cells due to disease or trauma is an irreversible process that can lead to blindness. Interestingly, regeneration of retinal neurons is a well established process in some non-mammalian vertebrates and is driven b...
Single-cell transcriptomic analysis of corneal organoids during development [0.03%]
角膜类器官发育过程中的单细胞转录组分析
Aditi Swarup,Ragini Phansalkar,Maurizio Morri et al.
Aditi Swarup et al.
Corneal organoids are useful tools for disease modeling and tissue transplantation; however, they have not yet been well studied during maturation. We characterized human iPSC-derived corneal organoids at 1, 2, 3, and 4 months of developmen...
Characterization of human pluripotent stem cell differentiation by single-cell dual-omics analyses [0.03%]
单细胞双组学分析描绘人多能干细胞分化特征
Daiyuan Liu,Yuting Fu,Xinru Wang et al.
Daiyuan Liu et al.
In vivo differentiation of human pluripotent stem cells (hPSCs) has unique advantages, such as multilineage differentiation, angiogenesis, and close cell-cell interactions. To systematically investigate multilineage differentiation mechanis...
Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 [0.03%]
神经源性蛋白2通过调控成人亚ependymal区神经前体细胞程序化表达谷氨酸能神经元谱系基因促进其向谷氨酸能神经元分化
Sophie Péron,Leo M Miyakoshi,Monika S Brill et al.
Sophie Péron et al.
Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 coul...
Deriving early single-rosette brain organoids from human pluripotent stem cells [0.03%]
从人多能干细胞中分离出早期单叶脑类器官
Andrew M Tidball,Wei Niu,Qianyi Ma et al.
Andrew M Tidball et al.
Brain organoid methods are complicated by multiple rosette structures and morphological variability. We have developed a human brain organoid technique that generates self-organizing, single-rosette cortical organoids (SOSR-COs) with reprod...
DOT1L interaction partner AF10 controls patterning of H3K79 methylation and RNA polymerase II to maintain cell identity [0.03%]
DOT1L相互作用蛋白AF10调控H3K79甲基化和RNA聚合酶II的模式以维持细胞身份
Coral K Wille,Edwin N Neumann,Aniruddha J Deshpande et al.
Coral K Wille et al.
Histone 3 lysine 79 methylation (H3K79me) is enriched on gene bodies proportional to gene expression levels and serves as a strong barrier for the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs). DOT1L is the sole h...