Single-cell multiomic comparison of mouse and rat spermatogenesis reveals gene regulatory networks conserved for over 20 million years [0.03%]
小鼠和大鼠精子发生的单细胞多组学比较揭示了超过2000万年进化中保守的基因调控网络
Eoin C Whelan,John J Swain,Jonathan H Sussman et al.
Eoin C Whelan et al.
Spermatogenesis is driven by dramatic changes in chromatin regulation, gene transcription, and protein expression. To assess the mechanistic bases for these developmental changes, we utilized multiomic single-cell/nucleus RNA sequencing (sc...
Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells [0.03%]
基因变异调节原始多能干细胞中DNA低甲基化和印记调控异常的易感性
C Parikh,R A Glenn,Y Shi et al.
C Parikh et al.
Naive pluripotent stem cells (nPSCs) frequently undergo pathological loss of DNA methylation at imprinted gene loci, posing a hurdle for biomedical applications and underscoring the need to identify underlying causes. We show that nPSCs fro...
Differentiation success of reprogrammed cells is heterogeneous in vivo and modulated by somatic cell identity memory [0.03%]
重编程细胞的分化成功率体内存在异质性并受体细胞身份记忆调节
Tomas Zikmund,Jonathan Fiorentino,Chris Penfold et al.
Tomas Zikmund et al.
Nuclear reprogramming can change cellular fates. Yet, reprogramming efficiency is low, and the resulting cell types are often not functional. Here, we used nuclear transfer to eggs to follow single cells during reprogramming in vivo. We sho...
Metabolic remodeling in hiPSC-derived myofibers carrying the m.3243A>G mutation [0.03%]
携带m.3243A>G突变的hiPSC来源肌纤维的代谢重塑
Gabriel E Valdebenito,Anitta R Chacko,Chih-Yao Chung et al.
Gabriel E Valdebenito et al.
Mutations in mitochondrial DNA cause severe multisystem disease frequently associated with muscle weakness. The m.3243A>G mutation is the major cause of mitochondrial encephalomyopathy lactic acidosis and stroke-like episodes (MELAS). Exper...
FOXO4-SP6 axis controls surface epithelium commitment by mediating epigenomic remodeling [0.03%]
FOXO4-SP6轴通过介导上皮表观基因组重塑控制表面上皮细胞的命运决定
Jiafeng Liu,Huaxing Huang,Fengjiao An et al.
Jiafeng Liu et al.
Proper development of surface epithelium (SE) is a requisite for the normal development and function of ectodermal appendages; however, the molecular mechanisms underlying SE commitment remain largely unexplored. Here, we developed a KRT8 r...
Robust generation of photoreceptor-dominant retinal organoids from porcine induced pluripotent stem cells [0.03%]
来自诱导多能干细胞的猪视网膜类器官的光感受器主导的稳健生成
Kimberly L Edwards,Bethany M Moore,Tyler-Serie Ganser et al.
Kimberly L Edwards et al.
Outer retinal degenerative diseases (RDDs) and injuries leading to photoreceptor (PR) loss are prevailing causes of blindness worldwide. While significant progress has been made in the manufacture of human pluripotent stem cell (hPSC)-deriv...
Salt-inducible kinases transduce mechanical forces into the specification of the pancreatic endocrine lineage [0.03%]
盐诱导激酶将机械力转化为胰腺内分泌谱系的特化过程中的信号传递
Chenglei Tian,Adam Rump,Christine Ebeid et al.
Chenglei Tian et al.
The extracellular matrix-F-actin-Yes-associated protein 1 (YAP1)-Notch mechanosignaling axis is a gatekeeper in the fate decisions of bipotent pancreatic progenitors (bi-PPs). However, the link between F-actin dynamics and YAP1 activity rem...
Safe CNV removal is crucial for successful hESC-RPE transplantation in wet age-related macular degeneration [0.03%]
安全去除CNV对于治疗湿性年龄相关性黄斑变性的hESC-RPE移植至关重要
Ying Xue Lv,Qi You Li,Ping Duan et al.
Ying Xue Lv et al.
Subretinal transplantation of human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells has demonstrated therapeutic potential in macular degeneration. However, its efficiency is limited in wet age-related macular degene...
High-dose radiation preferentially induces the clonal expansion of hematopoietic progenitor cells over mature T and B cells in mouse bone marrow [0.03%]
高剂量辐射优先诱导小鼠骨髓中造血祖细胞的克隆扩增而非成熟T细胞和B细胞
Kengo Yoshida,Munechika Misumi,Kanya Hamasaki et al.
Kengo Yoshida et al.
Radiation induces clonal hematopoiesis (CH) involving high-frequency somatic mutations in hematopoietic cells. However, the effects of radiation on clonal expansion of hematopoietic progenitor cells and lymphocytes remain elusive. Here, we ...
Cardiac differentiation roadmap for analysis of plasticity and balanced lineage commitment [0.03%]
心脏分化路线图在可塑性和平衡系谱承诺中的分析 Roads
Rebecca R Snabel,Carla Cofiño-Fabrés,Marijke Baltissen et al.
Rebecca R Snabel et al.
Stem cell-based models of human heart tissue and cardiac differentiation employ monolayer and 3D organoid cultures with different properties, cell type composition, and maturity. Here we show how cardiac monolayer, embryoid body, and engine...