Machine-Learning-Assisted Pathway Optimization in Large Combinatorial Design Spaces: A p-Coumaric Acid Case Study [0.03%]
机器学习辅助的路径优化在大型组合设计空间中的应用——以对羟基桂皮酸为例
Paul van Lent,Rianne van der Hoek,Sara Moreno Paz et al.
Paul van Lent et al.
Combinatorial pathway optimization is a powerful approach in metabolic engineering to improve strain performance. While machine learning (ML) has shown promise in guiding the Design-Build-Test-Learn (DBTL) cycle, most applications have been...
Complete Utilization of Poly(butylene Adipate- co-terephthalate) Monomers and Production of Polyhydroxybutyrate by a Levulinic Acid-Mediated Microbial Consortium of Pseudomonas putida and Cupriavidus necator [0.03%]
聚丁二酸-adipate-对苯二甲酸共聚酯单体的完全利用及Pseudomonas putida和Cupriavidus necator微生物 consortium介导生产聚羟基丁酸酯
Gaeun Lim,Yunhee Jeong,Seung Hun Lee et al.
Gaeun Lim et al.
Poly(butylene adipate-co-terephthalate) (PBAT) is a biodegradable plastic with enhanced mechanical properties, which can serve as a substitute for conventional plastics. However, the monomers released during its biodegradation, such as adip...
Plasmid- and Inducer-Free Biosynthesis of Difucosyllactose through Rational Fusion Enzyme Engineering and Systematic Pathway Optimization [0.03%]
通过理性融合酶工程技术及系统途径优化实现无质粒、无诱导物差加糖乳糖的生物合成
Zhiqiang Liu,Binglin Li,Jieying Deng et al.
Zhiqiang Liu et al.
Difucosyllactose (DFL), a fucosylated human milk oligosaccharide, exhibits significant application potential in the food and pharmaceutical industries. However, the microbial production of DFL faces challenges such as the accumulation of th...
Engineering Auronidin Production in Marchantia polymorpha Enables a New Class of Plant-Derived Textile Pigments [0.03%]
工程改造轮藻属植物大量生产花青素——一类新型的植物来源纺织染料
Aurore Lormet,Jim Haseloff,Facundo Romani
Aurore Lormet
Plant-derived pigments offer sustainable alternatives to synthetic colorants, yet their practical deployment in textiles is limited by restricted chemical diversity and low abundance. Liverworts represent a source of diverse chemical compou...
Biosensor-Based Detection and Quantification of Arsenic in Drinking Water [0.03%]
基于生物传感器的饮用水中砷的检测与定量分析
Oskar S Zeballos Huarachi,Andrea A Tambo Santos,Jonatan J Aliaga Rodriguez et al.
Oskar S Zeballos Huarachi et al.
Arsenic contamination of drinking water remains a persistent global health burden and an environmental justice challenge, particularly for low-resource communities that lack access to reliable monitoring tools. Synthetic-biology-driven bios...
A Programmable mRNA Platform for miRNA Detection via miRNA-mRNA2 Triplex-Mediated Ribosomal Frameshifting [0.03%]
一种可通过miRNA-mRNA2三联体介导的核糖体框移实现的miRNA检测的可编程mRNA平台
Yanyu Chen,Wanqi Zhao,Huizhen Chen et al.
Yanyu Chen et al.
Programmed -1 ribosomal frameshifting (-1 PRF) is a recoding mechanism utilized by viruses to expand their coding capacity and modulate the stoichiometric ratio of -1 frame and 0 frame translation products. The stability of mRNA secondary s...
Synthetic Intrinsically Disordered Proteins Enable Soluble Expression of Disulfide-Rich Therapeutics in E. coli [0.03%]
合成内在无序蛋白能使富含二硫键的治疗蛋白在大肠杆菌中可溶表达
Yulia Shmidov,Parul Sirohi,Jonathan C Su et al.
Yulia Shmidov et al.
We previously reported the de novo design of three small (
PeptideSGCL: Structure-Enhanced Graph-Transformer Encoding and Dual-Level Contrastive Learning for Peptide Property Prediction [0.03%]
基于结构增强的图变换编码和双层对比学习的肽性质预测模型
Jiajie Cai,Shuwen Xiong,Yuntao Yang et al.
Jiajie Cai et al.
Peptides play important roles in biological processes and biomedical applications, and their hemolytic (Hemo) and nonfouling (NF) properties directly affect their safety and translational potential. Therefore, accurate predictive models are...
Rational Engineering of a Sucrose Phosphorylase for Switchable Regioselective Synthesis of Antioxidant Gallic Acid α-Glucosides [0.03%]
用于合成抗氧化剂棓酸α-葡萄糖苷的可切换区域选择性变位酶的理性工程学研究
Zhiwei Li,Zijian Wang,Jingwei Guo et al.
Zhiwei Li et al.
Gallic acid is a bioactive polyphenol with poor solubility and stability. Enzymatic α-glucosylation can address these limitations, but regioselective synthesis among multiple phenolic hydroxyls remains challenging. Here, we engineer a sucr...
Heterologous Rieske Nonheme Iron Monooxygenases Enable Efficient Microbial Conversion of Lignin Guaiacol to Adipic Acid [0.03%]
异源类 Rif 型非血红素铁单氧酶催化的愈创木酚高效合成己二酸
John F C Steele,Charles B Wackwitz,Gary Walker et al.
John F C Steele et al.
Adipic acid (1,6-hexanedioic acid) is a key building block for nylon-6,6, a widely used polymer in the global chemical industry. Current industrial production relies on petrochemical feedstocks and nitric acid oxidation of cyclohexane/cyclo...