Mechanically knocking out titin reveals protein tension loss as a trigger of muscle disease [0.03%]
用机械方式敲除titin蛋白揭示蛋白张力丢失是肌肉疾病发病的诱因
Roberto Silva-Rojas,Natalia Vicente,Manuel Gavilán-Herrera et al.
Roberto Silva-Rojas et al.
Titin, the elastic protein scaffold of muscle sarcomeres, has multifunctional roles in mechanosignalling and is implicated in muscle disease. However, the consequences of disrupting titin's mechanical function in vivo remain incompletely un...
Microfluidics combined with electron microscopy for rapid and high-throughput mapping of antibody-viral glycoprotein complexes [0.03%]
结合电子显微镜的微流控技术实现抗体-病毒糖蛋白复合物的快速高效图谱绘制
Leigh M Sewall,Rebeca de Paiva Froes Rocha,Grace Gibson et al.
Leigh M Sewall et al.
Understanding the mechanistic interplay between antibodies and invading pathogens is essential for vaccine development. Current methods are labour and time intensive and limited by sample preparation bottlenecks. Here we present microfluidi...
NAD+ biosynthesis and mitochondrial repair in acute kidney injury via ultrasound-responsive thylakoid-integrating liposomes [0.03%]
一种声敏类囊体整合型脂质体制剂用于急性肾损伤的烟酸胺腺嘌呤二核苷酸合成及线粒体修复研究
Yao Lei,Yuzhu Wu,Wan-Ru Zhuang et al.
Yao Lei et al.
Acute kidney injury (AKI) impairs the energy metabolism and antioxidant capacity of renal proximal tubular cells. Here we show that ultrasound-responsive liposomes integrating thylakoid fragments and encapsulating L-ascorbic acid can restor...
Marc W Slutzky,Mariska J Vansteensel,Christian Herff et al.
Marc W Slutzky et al.
Jess D Hebert,Haiqing Xu,Yuning J Tang et al.
Jess D Hebert et al.
Somatic genome editing in mouse models has increased our understanding of the in vivo effects of genetic alterations. However, existing models have a limited ability to create multiple targeted edits, hindering our understanding of complex ...
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection [0.03%]
一种灵敏且持久的生物发光免疫测定法用于检测病毒抗原
Sungwan Kim,Giwon Cho,Jaebaek Lee et al.
Sungwan Kim et al.
Bioluminescence holds notable promise as a modality in diagnostics due to its high signal-to-noise ratio and absence of incident radiation. However, challenges arise from rapid signal decay and reduced enzyme activity when linked to targeti...
Rapid clearance of achiral small-molecule drugs using de novo-designed proteins and their cyclic and mirror-image variants [0.03%]
从头设计的蛋白质及其环状和镜像变体用于快速清除非手性小分子药物
Guilin Chen,Kai Zhao,Mengjiao Li et al.
Guilin Chen et al.
Small-molecule-binding proteins can neutralize toxins and cytotoxic drugs, but their development is hindered by unpredictable in vivo behaviour and the poor immunogenicity of small molecules, which limits antibody-based approaches. Here we ...
ROCK-dependent mechanotransduction of macroscale forces drives fibrosis in degenerative spinal disease [0.03%]
依赖ROCK的机械转导宏观力驱动退行性脊柱疾病的纤维化
Muhamed Hadzipasic,Margaret S Sten,Elie Massaad et al.
Muhamed Hadzipasic et al.
Chronic repetitive forces on the spinal column promote the development of degenerative spinal disease. Yet the mechanisms linking such macroscale mechanical forces to tissue hypertrophy remain unknown. Here we show that fibrotic regions in ...
The cerebellum shapes motions by encoding motor frequencies with precision and cross-individual uniformity [0.03%]
精确而统一地编码运动频率塑造小脑运动模式
Chia-Wei Liu,Yi-Mei Wang,Shun-Ying Chen et al.
Chia-Wei Liu et al.
Understanding brain behaviour encoding or designing neuroprosthetics requires identifying precise, consistent neural algorithms across individuals. However, cerebral microstructures and activities are individually variable, posing challenge...