Trichostatin A modulates apoptotic-related gene expression and improves embryo viability in cloned bovine embryos [0.03%]
组蛋白去乙酰化酶抑制剂改善克隆胚胎基因表达和胚胎质量的研究
Xiang-Shun Cui,Yong-Nan Xu,Xing-Hui Shen et al.
Xiang-Shun Cui et al.
Low efficiency of somatic cell nuclear transfer (SCNT) is attributed to incomplete reprogramming of transferred nuclei into oocytes. Trichostatin A (TSA), a histone deacetylase inhibitor, has been used to enhance nuclear reprogramming follo...
DNA methylation patterns are appropriately established in the sperm of bulls generated by somatic cell nuclear transfer [0.03%]
体细胞克隆公牛精子甲基化模式的研究
Christine Couldrey,David N Wells,Rita S F Lee
Christine Couldrey
The cloning of animals by somatic cell nuclear transfer (SCNT) has the potential to allow rapid dissemination of desirable traits from elite animals. However, concern has been expressed that aberrant epigenetic marks in SCNT-derived animals...
Development of an optimized zona-free method of somatic cell nuclear transfer in the goat [0.03%]
山羊无 zona 皮层胞核移植受体卵母细胞的优化研究
M H Nasr-Esfahani,S M Hosseini,M Hajian et al.
M H Nasr-Esfahani et al.
The purpose of this study was to develop an improved zona-free method of goat somatic cell nuclear transfer (SCNT) that has both ease of operation and efficiency. The main steps involved were: (1) optimization of in vitro oocyte maturation,...
Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle [0.03%]
胞质在跨界体细胞克隆胚胎第一次细胞周期核及核仁重建中作用的研究
Olga Østrup,Frantisek Strejcek,Ida Petrovicova et al.
Olga Østrup et al.
Initially, development of the zygote is under control of the oocyte ooplasm. However, it is presently unknown if and to what extent is the ooplasm able to interact with a transferred somatic cell from another species in the context of inter...
Pluripotent male germline stem cells from goat fetal testis and their survival in mouse testis [0.03%]
来源于山羊胎儿睾丸的多能雄性生殖干细胞及其在小鼠睾丸中的存活
Jinlian Hua,Haijing Zhu,Shaohui Pan et al.
Jinlian Hua et al.
Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both i...
Reprogramming of mouse fibroblasts to an intermediate state of differentiation by chemical induction [0.03%]
小分子诱导鼠成纤维细胞重编程至分化中间状态
Joonghoon Park,Chul Kim,Yong Tang et al.
Joonghoon Park et al.
Induced pluripotent stem cells (iPSCs) generated by forced expression of four transcription factors offer promises for regenerative and therapeutic uses in human diseases. However, it is necessary to overcome the risk of tumorigenicity caus...
Gene expression profiles and metabolic changes in embryonic neural progenitor cells under low oxygen [0.03%]
低氧条件下胚胎神经干/前体细胞的基因表达谱和代谢变化分析
Ling-Ling Zhu,Tong Zhao,Xin huang et al.
Ling-Ling Zhu et al.
Hypoxia promotes the proliferation of neural progenitor cells (NPCs), and low oxygen is a useful tool for expansion of NPCs in vitro. To further understand the regulation of the mechanisms involved, we first identified the gene expression p...
Functionality and transduction condition evaluation of recombinant Klf4 for improved reprogramming of iPS cells [0.03%]
重组Klf4的功能和转导条件评估以提高诱导多能干细胞的重编程效率
Yong Tang,Chih-Jen Lin,X Cindy Tian
Yong Tang
The induced pluripotent stem cell (iPSC) technology holds great potential in regenerative therapy. iPSCs could be induced by proteins (piPSC) linked with poly-arginine cell-penetrating peptides (CPPs) without the risk of genomic alteration,...
Comparison of three embryo culture methods for derivation of human embryonic stem cells from discarded embryos [0.03%]
比较三种胚胎培养方法以从废弃的胚胎中获取人类胚胎干细胞的效果
Ying Liu,Yang Li,Andrew Hwang et al.
Ying Liu et al.
Human embryonic stem cells (hESC) are self-renewing and pluripotent cells that hold great promise. Our objective was to compare the effect of three different embryo culture methods for derivation of human embryonic stem cells from discarded...
Comparative Study
Cellular reprogramming. 2011 Jun;13(3):233-9. DOI:10.1089/cell.2010.0092 2011
Hui-Li Wang,Zhong-Le Chang,Kun-Liang Li et al.
Hui-Li Wang et al.
The removal of chromosomes from recipient oocytes is one of the key steps in nuclear transfer cloning. Although microtubule interrupters have been successfully used for oocyte enucleation, their potential side effect on oocyte developmental...
Comparative Study
Cellular reprogramming. 2011 Jun;13(3):225-32. DOI:10.1089/cell.2010.0101 2011