Adam Thibodeaux,Guanhong Bu,Lael C Edwards et al.
Adam Thibodeaux et al.
Interest in electron diffraction (ED) for structural characterization of both proteins and small molecules has grown significantly over the last decade. While ab initio phasing methods remain the gold standard for ED data from small-molecul...
Structural insights into MltB from Acinetobacter baumannii: a conserved catalytic tunnel with unique domain arrangement [0.03%]
Hyunseok Jang,Chang Min Kim,Chang Sup Lee et al.
Hyunseok Jang et al.
Lytic transglycosylases (LTs) play a central role in bacterial cell wall remodeling by cleaving the β-1,4-glycosidic bond in peptidoglycan via a non-hydrolytic mechanism, producing 1,6-anhydroMurNAc termini crucial for recycling and signal...
Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography [0.03%]
Joshua Hutchings,Daniel Ji,Shawn Zheng et al.
Joshua Hutchings et al.
The Volta phase plate (VPP) can enhance low-frequency phase contrast in cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET), but its impact on tomogram alignment has not been systematically assessed. Using sub-2 Å sin...
Probing laser-driven surface and subsurface dynamics via grazing-incidence XFEL scattering and diffraction [0.03%]
基于斜入射X射线自由电子激光散射和衍射的飞秒强激光驱动表/界面及次表面动力学探测研究
Lisa Randolph,Özgül Öztürk,Dmitriy Ksenzov et al.
Lisa Randolph et al.
We demonstrate a grazing-incidence X-ray platform that simultaneously records time-resolved grazing-incidence small-angle X-ray scattering (GISAXS) and grazing-incidence X-ray diffraction (GID) from a femtosecond-laser-irradiated gold film ...
AXIS: a Lab-in-the-Loop machine learning approach for automated detection of macromolecular crystals [0.03%]
轴线：一种用于自动检测大分子晶体的闭环机器学习方法
Aurelien Personnaz,Sihyun Sung,Raphael Bourgeas et al.
Aurelien Personnaz et al.
Macromolecular crystallography provides mechanistic understanding of biological processes and can be applied in drug design. Nowadays, the use of robotic systems for crystal growth and diffraction analysis is widespread and high-throughput ...
Stéphanie Kodjikian
Stéphanie Kodjikian
Now a common technique in crystallography, three-dimensional electron diffraction (3D ED) is on track to bridge the gap with X-ray diffraction in terms of structural accuracy. ...
Kinetic effects yield different results on the timescales of laboratory and synchrotron high-pressure experiments [0.03%]
力学效应导致实验室和同步辐射高压实验时间尺度上的结果不同
Julien Haines
Julien Haines
Kinetic effects can be a critical factor in the study of high-pressure phase transitions. X-ray diffraction experiments on the timescales of the laboratory and the synchrotron can provide complementary results on such transformations. ...
Jon Cooper
Jon Cooper
The paper by Gilski et al. in this issue [IUCrJ (2026), 13, 132-145] focuses on the stereochemical analysis of the catalytic mechanism of asparaginases with the aim of resolving key ambiguities in the mechanism. These enzymes have found tru...
Mauro Gemmi,Lukáš Palatinus,Philippe Boullay et al.
Mauro Gemmi et al.
3D electron diffraction (3D ED) has undergone impressive development in the last decade. However, its accuracy and reproducibility have never been tested, up to now, in different laboratories on the same batch of samples. This paper reports...
Modelling of radiation damage and beam-induced heating of room-temperature samples at extremely high flux MX beamlines [0.03%]
极高科技含量的MX光束线室温样品的辐射损伤及光束引起的加热模拟研究
Martin V Appleby,Michal W Kepa,Graeme Winter et al.
Martin V Appleby et al.
The upgrade of the third-generation synchrotrons to diffraction-limited storage rings will enable a gain of up to two orders of magnitude in brilliance and further enable the creation of multiple macromolecular crystallography (MX) beamline...