Clonal evolution in serially passaged Cryptococcus neoformans × deneoformans hybrids reveals a heterogenous landscape of genomic change [0.03%]
连续传代的隐球菌与新生隐球菌杂交种的克隆演化揭示了基因组变化的异质景观
Lucas A Michelotti,Sheng Sun,Joseph Heitman et al.
Lucas A Michelotti et al.
Cryptococcus neoformans × deneoformans hybrids (also known as serotype AD hybrids) are basidiomycete yeasts that are common in a clinical setting. Like many hybrids, the AD hybrids are largely locked at the F1 stage and are mostly unable t...
TORC1 signaling modulates Cdk8-dependent GAL gene expression in Saccharomyces cerevisiae [0.03%]
TORC1信号转导调节酿酒酵母中依赖Cdk8的GAL基因表达
Riley Horvath,Nicole Hawe,Cindy Lam et al.
Riley Horvath et al.
Cdk8 of the RNA polymerase II mediator kinase complex regulates gene expression by phosphorylating sequence-specific transcription factors. This function is conserved amongst eukaryotes, but the signals and mechanisms regulating Cdk8 activi...
Properties and unbiased estimation of F- and D-statistics in samples containing related and inbred individuals [0.03%]
包含相关和近交个体的样本中F统计量和D统计量的属性及无偏估计
Mehreen R Mughal,Michael DeGiorgio
Mehreen R Mughal
The Patterson F- and D-statistics are commonly used measures for quantifying population relationships and for testing hypotheses about demographic history. These statistics make use of allele frequency information across populations to infe...
CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans [0.03%]
CeLINC，一种在秀丽隐杆线虫中基于荧光的蛋白质-蛋白质相互作用测定方法
Jason R Kroll,Sanne Remmelzwaal,Mike Boxem
Jason R Kroll
Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein's function. We present Caenorhabditis elegans light-induced cocluste...
Single nucleotide substitutions effectively block Cas9 and allow for scarless genome editing in Caenorhabditis elegans [0.03%]
单核苷酸替代可以有效阻止Cas9并允许在秀丽隐杆线虫中进行无痕基因组编辑
Jeffrey C Medley,Shilpa Hebbar,Joel T Sydzyik et al.
Jeffrey C Medley et al.
In Caenorhabditis elegans, germline injection of Cas9 complexes is reliably used to achieve genome editing through homology-directed repair of Cas9-generated DNA breaks. To prevent Cas9 from targeting repaired DNA, additional blocking mutat...
uS5/Rps2 residues at the 40S ribosome entry channel enhance initiation at suboptimal start codons in vivo [0.03%]
在体内增强次优起始密码子启动的40S核糖体入口通道的uS5/Rps2残基
Jinsheng Dong,Alan G Hinnebusch
Jinsheng Dong
The eukaryotic 43S pre-initiation complex (PIC) containing Met-tRNAiMet in a ternary complex (TC) with eIF2-GTP scans the mRNA leader for an AUG codon in favorable "Kozak" context. AUG recognition triggers rearrangement of the PIC from an o...
Wing hearts in four-winged Ultrabithorax-mutant flies-the role of Hox genes in wing heart specification [0.03%]
四翼Ultrabithorax突变果蝇的翼心--Hox基因在翼心特化中的作用
Markus Tögel,Günther Pass,Achim Paululat
Markus Tögel
Wings are probably the most advanced evolutionary novelty in insects. In the fruit fly Drosophila melanogaster, proper development of wings requires the activity of so-called wing hearts located in the scutellum of the thorax. Immediately a...
Intermittent fasting and caloric restriction interact with genetics to shape physiological health in mice [0.03%]
间歇性禁食和热量限制相互作用以影响小鼠的生理健康
Guozhu Zhang,Andrew Deighan,Anil Raj et al.
Guozhu Zhang et al.
Dietary interventions can dramatically affect physiological health and organismal lifespan. The degree to which organismal health is improved depends upon genotype and the severity of dietary intervention, but neither the effects of these f...
Short homology-directed repair using optimized Cas9 in the pathogen Cryptococcus neoformans enables rapid gene deletion and tagging [0.03%]
使用优化的Cas9进行短同源介导修复在病原真菌新生隐球菌中实现快速基因敲除和标记
Manning Y Huang,Meenakshi B Joshi,Michael J Boucher et al.
Manning Y Huang et al.
Cryptococcus neoformans, the most common cause of fungal meningitis, is a basidiomycete haploid budding yeast with a complete sexual cycle. Genome modification by homologous recombination is feasible using biolistic transformation and long ...
Genomic characterization of a wild diploid isolate of Saccharomyces cerevisiae reveals an extensive and dynamic landscape of structural variation [0.03%]
Saccharomyces cerevisiae野生二倍体菌株的基因组特征揭示了广泛的结构变异动态景观
Lydia R Heasley,Juan Lucas Argueso
Lydia R Heasley
The budding yeast Saccharomyces cerevisiae has been extensively characterized for many decades and is a crucial resource for the study of numerous facets of eukaryotic biology. Recent whole genome sequence analysis of over 1000 natural isol...