Solo regulates the localization and activity of LARG for actin cytoskeletal remodeling in cell-substrate adhesions [0.03%]
SOLO调控LARG在细胞粘附中的定位和活性从而重塑肌动蛋白细胞骨架
Aoi Kunitomi,Yusuke Toyofuku,Chikaya Fujimori et al.
Aoi Kunitomi et al.
In response to external mechanical stimuli, cells remodel their actin cytoskeleton. Solo, a Rho guanine nucleotide exchange factor (RhoGEF), is involved in mechanical stress responses via cell-substrate adhesions. Using BioID, we identified...
Fission yeast cells use distinct cell size control mechanisms to regulate cell geometry in response to osmotic, oxidative, or low glucose conditions [0.03%]
芽殖酵母细胞利用不同的细胞大小控制机制来响应渗透压、氧化或低葡萄糖条件调节细胞几何形状
Elena J Cabral,Pablo Andres,Geraldin Argandona et al.
Elena J Cabral et al.
Cells maintain an appropriate size to function, yet the mechanisms that enable size adaptation to environmental stress remain poorly understood. Fission yeast cells enter mitosis and divide at a threshold size when cyclin-dependent kinase (...
Engineered interfaces in Rac1 and Cdc42 biosensors enhance sensitivity and reduce cell perturbation [0.03%]
用于Rac1和Cdc42的生物传感器中工程化界面的设计可提高灵敏度并减少对细胞的影响
Daniel J Marston,Ellen C O&#x;Shaughnessy,Timothy M Jacobs et al.
Daniel J Marston et al.
Fluorescent biosensors are a valuable means to report the spatiotemporal dynamics of protein activities in live cells and animals. However, biosensors affect the activities they are reporting. This can be ameliorated by increasing sensitivi...
DYN-1 regulates SPD-2 and PLK-1 localization and mitotic spindle pole organization [0.03%]
DYN-1调控SPD-2和PLK-1定位及有丝分裂纺锤体极组织
Carter Dierlam,Stephanie Held,Jiya Hastings et al.
Carter Dierlam et al.
Accurate mitosis is essential for genome stability. Although dynamin is best known for its role in vesicular trafficking, its functions during mitosis remain unclear. Here, we identify a mitotic role for the C. elegans dynamin homolog DYN-1...
Bernardo Chapa-Y-Lazo,Thamarailingam Athilingam,Prabhat Tiwari et al.
Bernardo Chapa-Y-Lazo et al.
The use of fluorescent proteins has become ubiquitous throughout the life sciences as a key method for understanding molecular, cellular and tissue scale processes. Genetically encodable fluorophores have enabled stable genetic lines to be ...
Characterization of Orsay virus replication intermediates in Caenorhabditis elegans reveals links to antiviral RNA interference [0.03%]
在秀丽隐杆线虫中表征Orsay病毒复制中间体揭示了其与抗病毒RNA干扰的关系
Supraja Ranganathan,P Joseph Aruscavage,Brenda L Bass
Supraja Ranganathan
Orsay Virus (OV) is a positive-sense, single-stranded RNA (+ssRNA) virus that naturally infects C. elegans intestines. Like other +ssRNA viruses, the OV-encoded RNA-dependent RNA polymerase (oRdRP) synthesizes complementary antigenome for u...
Membrane composition-dependent patterning of Rho and F-actin in an artificial cell cortex [0.03%]
人工细胞皮层中Rho和F-actin的模式化依赖于膜组成
Gregory J Schwarz,Joanna R Suber,Devika Andhare et al.
Gregory J Schwarz et al.
Cortical excitability, a phenomenon in which the cell cortex is dynamically patterned with waves of F-actin assembly, has been described in a variety of model systems, including embryos of mammals, flies, frogs and echinoderms, as well as a...
Spatial Coin-Tossing by Kinesin-1 Head and Tail Binding Collectively Drives Microtubule Patterns [0.03%]
Kinesin-1的头部和尾部结合的时空随机性共同驱动微管模式化
Jashaswi Basu,Kajal Singh,Anita Jannasch et al.
Jashaswi Basu et al.
Intracellular microtubule-based transport depends on an essential plus-ended molecular motor, kinesin-1. The N-terminal ATP-dependent head driving motility and a C-terminal cargo interacting tail, both bind microtubules. Previously, the int...
5´Untranslated regions provide a versatile toolkit for tunable exogenous protein expression [0.03%]
5'非翻译区提供了一套多功能工具包以实现外源蛋白的可调式表达
Camila Garcia,Dylan Poch,Aden M Alemayhu et al.
Camila Garcia et al.
Transient transfection is widely used for protein expression in heterologous systems, yet uncontrolled overexpression frequently introduces artifacts that confound functional analyses. Although stable cell lines can mitigate these issues, g...
Differential functions of human DNA polymerase delta with p12 variants during DNA replication and DNA damage response [0.03%]
人DNA聚合酶delta与p12变体在DNA复制和DNA损伤应答中的不同功能
Jugal Kishor Sahu,Ipsita Subhadarsini,Shweta Thakur et al.
Jugal Kishor Sahu et al.
The function of p12, the smallest subunit of human DNA polymerase delta (Polδ), in DNA replication and DNA damage response (DDR) is poorly understood. Due to the identification of two protein encoding p12 isoforms, the respective Polδ var...