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FRET-based analysis of guanine nucleotide binding to γ-tubulin

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γ-Tubulin assembles into the γ-tubulin ring complex (γTuRC) that acts as the primary nucleator of cellular microtubules. γ-Tubulin binds Mg²⁺-associated GTP or GDP in an exchangeable manner; however, the nature of its GTP/GDP-binding properties has remai... ...