Techniques for the isolation of high-quality RNA from cells encapsulated in chitosan hydrogels [0.03%]
从壳聚糖水凝胶中包封的细胞分离高质量RNA的技术
Claire Yu,Stuart Young,Valerio Russo et al.
Claire Yu et al.
Extracting high-quality RNA from hydrogels containing polysaccharide components is challenging, as traditional RNA isolation techniques designed for cells and tissues can have limited yields and purity due to physiochemical interactions bet...
An improved cryosection method for polyethylene glycol hydrogels used in tissue engineering [0.03%]
一种改进的用于组织工程聚乙二醇水凝胶切片的方法研究
Jia-Ling Ruan,Nathaniel L Tulloch,Veronica Muskheli et al.
Jia-Ling Ruan et al.
The high water content of hydrogels allows these materials to closely mimic the native biological extracellular conditions, but it also makes difficult the histological preparation of hydrogel-based bioengineered tissue. Paraffin-embedding ...
Macromolecular crowding effect on cartilaginous matrix production: a comparison of two-dimensional and three-dimensional models [0.03%]
高分子拥挤效应对软骨基质生成的影响：二维和三维模型的比较
Bo Chen,Bin Wang,Wen Jie Zhang et al.
Bo Chen et al.
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Fi...
Comparative Study
Tissue engineering. Part C, Methods. 2013 Aug;19(8):586-95. DOI:10.1089/ten.tec.2012.0408 2013
A dual flow bioreactor with controlled mechanical stimulation for cartilage tissue engineering [0.03%]
一种用于软骨组织工程的可控机械刺激双流式生物反应器
Tim W G M Spitters,Jeroen C H Leijten,Filipe D Deus et al.
Tim W G M Spitters et al.
In cartilage, tissue engineering bioreactors can create a controlled environment to study chondrocyte behavior under mechanical stimulation or produce chondrogenic grafts of clinically relevant size. Here we present a novel bioreactor that ...
Microfluidic enrichment of mouse epidermal stem cells and validation of stem cell proliferation in vitro [0.03%]
用于小鼠表皮干细胞富集的微流控芯片及其体外增殖能力验证
Beili Zhu,James Smith,Martin L Yarmush et al.
Beili Zhu et al.
Bulge stem cells reside in the lowest permanent portion of hair follicles and are responsible for the renewal of these follicles along with the repair of the epidermis during wound healing. These cells are identified by surface expression o...
Evangelia Bellas,Kacey G Marra,David L Kaplan
Evangelia Bellas
The need for physiologically relevant sustainable human adipose tissue models is crucial for understanding tissue development, disease progression, in vitro drug development and soft tissue regeneration. The coculture of adipocytes differen...
Capillary force seeding of sphere-templated hydrogels for tissue-engineered prostate cancer xenografts [0.03%]
毛细管力引导的微球模板水凝胶的种子细胞播种用于组织工程前列腺癌异种移植模型
Thomas J Long,Marc Takeno,Cynthia C Sprenger et al.
Thomas J Long et al.
Biomaterial-based tissue-engineered tumor models are now widely used in cancer biology studies. However, specific methods for efficient and reliable cell seeding into these and tissue-engineering constructs used for regenerative medicine of...
The utilization of perforated bioinert chambers to generate an in vivo isolated space for tissue engineering involving chondrocytes, mesenchymal stem cells, and fibroblasts [0.03%]
有关软骨细胞、间充质干细胞和成纤维细胞的组织工程体内隔离空间的构建及利用研究
Shiping Wang,Xiaoyan Hao,Yingjun Su et al.
Shiping Wang et al.
Vascular invasion and admixture of the nude mouse cells with seeded cells make it difficult to reapply the regenerated tissues to the restoration of host tissue defects. Therefore, a device that is capable of allowing for autologous or allo...
Differentiation of human embryonic stem cells using size-controlled embryoid bodies and negative cell selection in the production of photoreceptor precursor cells [0.03%]
利用大小控制型胚胎样体和负向细胞筛选技术诱导人胚胎干细胞向视网膜感光神经元方向分化的研究
Anat Yanai,Christopher R J Laver,Aaron W Joe et al.
Anat Yanai et al.
We proposed to optimize the retinal differentiation protocols for human embryonic stem cells (hESCs) by improving cell handling. To improve efficiency, we first focused on the production of just one retinal precursor cell type (photorecepto...
Development of an improved three-dimensional in vitro intestinal mucosa model for drug absorption evaluation [0.03%]
一种改进的体外三维肠道黏膜模型的建立及其在药物吸收评价中的应用研究
Na Li,Dandan Wang,Zhigang Sui et al.
Na Li et al.
Human epithelial cell culture models of monolayer Caco-2 cells have been widely employed to assess the absorption of drug molecules across intestinal mucosa. However, cautions should be taken when interpreting the conclusions from those mod...