Preparation of Human Primary Colon Tissue-Derived Organoid Using Air Liquid Interface Culture [0.03%]
空气液界面培养法建立人源性结肠类器官细胞系
Tatsuya Usui,Masashi Sakurai,Koji Umata et al.
Tatsuya Usui et al.
In vitro analysis of intestinal epithelium has been hindered by a lack of suitable culture systems useful for gastrointestinal research. To overcome the problem, an air liquid interface (ALI) method using a collagen gel was established to c...
Phenotypic and Functional Evaluation of Hematopoietic Stem and Progenitor Cells in Toxicology of Heavy Metals [0.03%]
毒理学中重金属造血干细胞和祖细胞的表型和功能评价
Qian Li,Zhengli Yang,Yifan Zhao et al.
Qian Li et al.
All blood cells are differentiated from hematopoietic stem and progenitor cells (HSPCs), a process known as hematopoiesis that can be influenced by a variety of environmental factors. In this unit, we introduce a couple of protocols includi...
Metallic Nano-Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers [0.03%]
利用斑马鱼胚胎毒性试验鉴定特定暴露生物标志物对金属纳米复合材料的毒性评估
Roberta Pecoraro,Antonio Salvaggio,Fabio Marino et al.
Roberta Pecoraro et al.
Nanomaterials (NM) have different shapes and can be composed of different materials such as carbon, silicon, and some metals like gold, silver, and titanium. They are used as fillers, catalysts, semiconductors, cosmetics, drug carriers in m...
Measuring β-Galactosidase Activity in Gram-Positive Bacteria Using a Whole-Cell Assay with MUG as a Fluorescent Reporter [0.03%]
基于MUG荧光报告的金黄色葡萄球菌β-半乳糖苷酶活性快速测定法研究
Norman H L Chiu,Amanda L Watson
Norman H L Chiu
The use of β-galactosidase enzyme as a biomarker has the potential to determine activity levels of the microbiome of a variety of organisms due to its common presence in both eukaryotes and prokaryotes. Completing the assay in a whole-cell...
Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells [0.03%]
人外周血单个核细胞的分离、冻存及免疫分型
Fredine T Lauer,Jesse L Denson,Scott W Burchiel
Fredine T Lauer
This unit describes procedures for the isolation, cryopreservation, and thawing of human peripheral blood mononuclear cells (HPBMC) and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This me...
Overview of Neurotoxicology [0.03%]
毒理学概论
Lucio G Costa
Lucio G Costa
The nervous system has a central and primary function in the body, and its relevance and complexity make it a target for a large number of toxic substances. The most common forms of neurotoxicity are the death of neurons (neuronopathy), the...
Pamela J Roqué,Lucio G Costa
Pamela J Roqué
Microglia, the resident immune cells of the brain, have been implicated in numerous neurodegenerative and neurodevelopmental diseases. Activation of microglia by a variety of stimuli induces the release of factors, including pro- and anti-i...
Inter-and Intra-Laboratory Standardization of TUNEL Assay for Assessment of Sperm DNA Fragmentation [0.03%]
用于评估精子DNA碎片的TUNEL测定的室间和室内标准化
Sajal Gupta,Rakesh Sharma,Ashok Agarwal
Sajal Gupta
The functional aspects of sperm activity such as sperm chromatin integrity and ability to fertilize cannot be characterized by routine semen parameters. Men with unexplained infertility and idiopathic infertility, as well as men with normoz...
Gas Chromatography Mass Spectrometry (GC-MS) for Identification of Designer Stimulants Including 2C Amines, NBOMe Compounds, and Cathinones in Urine [0.03%]
用于识别尿液中包括2C胺类、NBOMe类和卡西酮类在内的新型刺激物质的气相色谱-质谱联用(GC-MS)法
Hemamalini Ketha,Milad Webb,Larry Clayton et al.
Hemamalini Ketha et al.
Phenethylamine derivatives are being increasingly exploited for recreational use as "designer" stimulants designed to mimic psychostimulant properties of amphetamine or other illicit substances like 3,4-methylenedioxymethamphetamine (MDMA [...
Intracellular Cytokine Detection by Flow Cytometry in Surface Marker-Defined Human Peripheral Blood Mononuclear T Cells [0.03%]
基于表面标记的人外周血单个核细胞中t细胞的流式细胞检测方法及应用
Fredine T Lauer,Jesse L Denson,Ellen Beswick et al.
Fredine T Lauer et al.
In a recent unit in this series, protocols for the isolation, cryopreservation, thawing, and immunophenotyping of HPBMC isolated from peripheral whole blood using cell surface marker (CSM) staining and multi-color flow cytometry analysis we...