Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells
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This unit describes procedures for the isolation, cryopreservation, and thawing of human peripheral blood mononuclear cells (HPBMC) and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be use... ...