Sperm-Binding Assay Using an In Vitro 3D Model of the Mammalian Cumulus-Oocyte Complex [0.03%]
利用体外三维培养的哺乳动物放射冠-卵母细胞复合物模型进行精子结合试验
Julieta Gabriela Hamze,María Jiménez-Movilla,Raquel Romar
Julieta Gabriela Hamze
We have recently described a new model to study gamete interaction in mammalian species. The model recreates the spherical surface of the oocyte by using magnetic Sepharose beads coated with a layer of a recombinant protein involved in game...
Using Human Primary Foreskin Fibroblasts to Study Cellular Damage and Mitochondrial Dysfunction [0.03%]
人包皮成纤维细胞的细胞损伤及线粒体功能障碍研究
Cristina A Nadalutti,Samuel H Wilson
Cristina A Nadalutti
Several cell lines of different origin are routinely used in research and drug development as important models to study human health and disease. Studying cells in culture represents an easy and convenient tool to approach complex biologica...
An Open-Globe Porcine Injury Platform for Assessing Therapeutics and Characterizing Biological Effects [0.03%]
一种开放式猪眼损伤平台，用于评估治疗措施和表征生物效应
Eric J Snider,Peter R Edsall,Lauren E Cornell et al.
Eric J Snider et al.
Open-globe injuries can result in permanent vision loss, partly due to extended delays between injury and medical intervention. Even with early intervention, the management of open-globe injuries remains a challenge for ophthalmologists, mo...
A Protocol to Study Mitochondrial Function in Human Neural Progenitors and iPSC-Derived Astrocytes [0.03%]
研究人类神经前体和iPSC来源星形胶质细胞的线粒体功能的protocol
Gabriela Assis-de-Lemos,Pítia Flores Ledur,Karina Karmirian et al.
Gabriela Assis-de-Lemos et al.
Mitochondrial dysfunction is a central component in the pathophysiology of multiple neuropsychiatric and degenerative disorders. Evaluating mitochondrial function in human-derived neural cells can help characterize dysregulation in oxidativ...
Measuring Changes in Keap1-Nrf2 Protein Complex Conformation in Individual Cells by FLIM-FRET [0.03%]
通过FLIM-FRET测量单个细胞中Keap1-Nrf2蛋白复合物构象的变化
Dina Dikovskaya,Albena T Dinkova-Kostova
Dina Dikovskaya
The nuclear factor-erythroid 2 p45-related factor 2 (Nrf2)-mediated stress response is a major cellular defense mechanism against endogenous and exogenous oxidants, electrophiles, and pro-inflammatory agents. A number of Nrf2 inducers are b...
Jane Kasten-Jolly,David A Lawrence
Jane Kasten-Jolly
Culture of human peripheral blood mononuclear cells (PBMCs) still remains a convenient and sensitive method for measurement of a person's immune system health. Basic elements of the process, namely PBMC purification and culture medium formu...
Analysis of Busulfan in Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) [0.03%]
液相色谱-串联质谱法测定血液中巴利夫仑的浓度分析
Abed Pablo,Autumn R Breaud,William Clarke
Abed Pablo
Bone marrow transplantation is used to treat particular types of cancers such as lymphoma, leukemia, and multiple myeloma. Appropriate dosing of busulfan during the preparative phase is critical for a successful allograft; if blood concentr...
Generating Bacterial Foods in Toxicology Studies with Caenorhabditis elegans [0.03%]
利用秀丽隐杆线虫进行毒理学研究时细菌食物的生成
Tao Ke,Abel Santamaría,Alexey A Tinkov et al.
Tao Ke et al.
Caenorhabditis elegans is a free-living animal that is used as a powerful experimental model in biological sciences. The natural habitat of the animal are areas rich in material from rotting plants or fruits being decomposed by a growing nu...
Analysis of Immunosuppressant Drugs in Whole Blood by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) [0.03%]
液相色谱-串联质谱法测定全血免疫抑制剂药物浓度分析方法探讨
Abed H Pablo,Autumn R Breaud,William Clarke
Abed H Pablo
Immunosuppressant medications help suppress the immune system response through inhibition of various checkpoints in the regulatory biochemical pathway. This is useful in prevention of organ rejection in transplantation or in the treatment o...
A Functional Assay to Assess Toxicity During Murine B Cell Development In Vitro [0.03%]
评估小鼠B细胞体外毒性的一种功能测定法
Cynthia Guilbert,Hsiang Chou,Alicia M Bolt et al.
Cynthia Guilbert et al.
B lymphocytes, or B cells, are important players in immunity that produce antigen-specific immunoglobulins. As a result, they are involved in various immune-linked pathologies. To better understand, prevent, or treat B cell-associated disea...