Protein aggregation and polyasparagine-mediated cellular toxicity in Saccharomyces cerevisiae [0.03%]
酿酒酵母中的蛋白质聚集及聚多天冬氨酸介导的细胞毒性
Theodore W Peters,Mingxia Huang
Theodore W Peters
It is well established that protein aggregation is associated with many neurodegenerative disorders including polyglutamine diseases, but a mechanistic understanding of the role of protein aggregates in the disease pathogenesis remains elus...
M Teresa Pastor,Alexandra Esteras-Chopo,Luis Serrano
M Teresa Pastor
Many research efforts in the last years have been directed towards understanding the factors determining protein misfolding and amyloid formation. Protein stability and amino acid composition have been identified as the two major factors in...
Annalisa Pastore,Adriana Zagari
Annalisa Pastore
Among the diseases caused by protein misfolding is the family associated with the prion protein (PrP). This is a small extracellular membrane-anchored molecule of yet unknown function. Understanding how PrP folds both into its cellular and ...
Interplays between covalent modifications in the endoplasmic reticulum increase conformational diversity in nascent prion protein [0.03%]
内质网中蛋白质共价修饰之间的相互作用可增加新合成的朊毒蛋白构象的多样性
Andrea Orsi,Roberto Sitia
Andrea Orsi
Prion protein (PrP), the causative agent of transmissible spongiform encephalopathies, is synthesized in the endoplasmic reticulum (ER) where it undergoes numerous covalent modifications. Here we investigate the interdependence and regulati...
Effects of mutations in yeast prion [PSI+] on amyloid toxicity manifested in Escherichia coli strain BL21 [0.03%]
酵母蛋白酶体丝状毒性因子[PSI+]突变在大肠杆菌BL21菌株中的效应研究
Bun-ichiro Ono,Hiroshi Kawaminami,Hironori Kobayashi et al.
Bun-ichiro Ono et al.
We previously showed that over production of a fusion protein in which the prion domain of Saccharomyces cerevisiae [PSI(+)] is connected to glutathione S-transferase (GST-Sup35NM) causes a marked decrease in the colony forming ability of E...
Screening of DNA aptamer against mouse prion protein by competitive selection [0.03%]
竞争性选择筛选针对小鼠朊蛋白的DNA适配体
Daisuke Ogasawara,Hijiri Hasegawa,Kiyotoshi Kaneko et al.
Daisuke Ogasawara et al.
Prion disease is a neurodegenerative disorder, in which the normal prion protein (PrP) changes structurally into an abnormal form and accumulates in the brain. There is a great demand for the development of a viable approach to diagnosis an...
Marie-Lise Maddelein
Marie-Lise Maddelein
Amyloid protein aggregation is involved in serious neurodegenerative disorders such as Alzheimer's disease and transmissible encephalopathies. The concept of an infectious protein (prion) being the scrapie agent was successfully validated f...
Tau aggregation in Alzheimer's disease: what role for phosphorylation? [0.03%]
tau蛋白聚集与阿尔茨海默病：磷酸化起到什么作用？
Guy Lippens,Alain Sillen,Isabelle Landrieu et al.
Guy Lippens et al.
The crucial role of the neuronal Tau protein in microtubule stabilization and axonal transport suggests that too little or too much Tau might lead to neuronal dysfunction. The presence of a hyper phosphorylated but non aggregated molecule a...
Toxic effects of intracerebral PrP antibody administration during the course of BSE infection in mice [0.03%]
PrP抗体在小鼠感染疯牛病过程中对脑内的毒性作用
Maxime Lefebvre-Roque,Elisabeth Kremmer,Sabine Gilch et al.
Maxime Lefebvre-Roque et al.
The absence of specific immune response is a hallmark of prion diseases. However, in vitro and in vivo experiments have provided evidence that an anti-PrP humoral response could have beneficial effects. Prophylactic passive immunization per...
Susceptibility of cell substrates to PrPSc infection and safety control measures related to biological and biotherapeutical products [0.03%]
细胞基质感染PrPSC的易感性和生物、生物治疗制品的相关安全控制措施
Matthew LeBrun,Hongsheng Huang,Xuguang Li
Matthew LeBrun
Concerns over the potential for infectious prion proteins to contaminate human biologics and biotherapeutics have been raised from time to time. Transmission of the pathogenic form of prion protein (PrP(Sc)) through veterinary vaccines has ...