Effects of mutations in yeast prion [PSI+] on amyloid toxicity manifested in Escherichia coli strain BL21
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We previously showed that over production of a fusion protein in which the prion domain of Saccharomyces cerevisiae [PSI(+)] is connected to glutathione S-transferase (GST-Sup35NM) causes a marked decrease in the colony forming ability of Escherichia coli stra... ...