A regenerative and programmable iPSC-derived platelet platform for universal drug delivery and immunotherapy [0.03%]
一种用于通用药物输送和免疫治疗的再生型和可编程的iPSC衍生血小板平台
Lingna Wang,Pengbo Zhang,Chen Wu et al.
Lingna Wang et al.
Platelet-based drug-delivery systems have garnered increasing attention for cancer treatment, yet their broader application is hindered by the limited source and poor genetic modifiability. Here, we established a regenerative and programmab...
Engineering single-dose plasmid DNA for sustained in vivo delivery of designer incretins [0.03%]
工程化质粒DNA以持续体内递送新型肠促胰素类药物
Ebony N Gary,Yangcheng Gao,Casey E Hojecki et al.
Ebony N Gary et al.
Incretin mimetic drugs have transformed the treatment of obesity and type 2 diabetes; however, production and patient compliance challenges remain. Plasmid DNA has recently demonstrated the ability to deliver functional monoclonal antibodie...
Microelectrode array technology for organoids and microphysiological systems [0.03%]
类器官和微生理系统中的微电极阵列技术
Alp T Toymus,Tawab Karim,Jean-Philippe Frimat et al.
Alp T Toymus et al.
Organoids and microphysiological systems (MPS) constitute relevant in vitro (patho)physiological models, complementary to two-dimensional (2D) cell cultures and animal studies, by recapitulating in vivo three-dimensional (3D) tissue microen...
Programmable mycolysins expand the antimicrobial toolkit against mycobacteria [0.03%]
可编程肉孢子虫抗生素扩大了针对分枝杆菌的抗菌工具箱
Carlos São-José,Madalena Pimentel
Carlos São-José
In a timely advance for antimycobacterial biotechnology, Abouhmad et al. engineered modular 'mycolysins' that combine mycobacteriophage lysis functions with membrane-active peptides to efficiently breach the mycobacterial envelope and achie...
Reprogramming bacterial energetics reverses lactate-mediated immunosuppression for synergistic cancer immunotherapy [0.03%]
逆转乳酸介导的免疫抑制的细菌癌症免疫疗法
Jing Zhao,Nuo Zhang,Lingfeng Qin et al.
Jing Zhao et al.
The immunosuppressive tumor microenvironment (TME), driven by lactate accumulation, critically limits cancer immunotherapy efficacy. In this research article, we engineered Shewanella oneidensis (S. oneidensis) MR-1 by reprogramming energy ...
Engineered Bacillus subtilis to deliver dsRNA via extracellular vesicles against the H9N2 avian influenza virus [0.03%]
工程化枯草芽孢杆菌通过胞外囊泡递送dsRNA抑制禽流感H9N2病毒
Jiachen Liu,Yimeng Yang,Yichao Ma et al.
Jiachen Liu et al.
RNA interference (RNAi) is a potent antiviral approach, outperforming traditional pesticides and broad-spectrum drugs. Its use in animal disease control faces two challenges: inefficient target design relying on computer-predicted small-int...
A modular all-in-one genetic engineering tool for the diatom Phaeodactylum tricornutum based on CRISPR-Cas12a [0.03%]
基于CRISPR-Cas12a的三角褐指藻一站式模块化基因工程技术
Nicola Trevisan,Christina Zotou,Rodrigo M Martins et al.
Nicola Trevisan et al.
The marine diatom Phaeodactylum tricornutum is a model microalgal species that is emerging as a platform for the photosynthetic production of industrial chemicals such as carotenoids and polyunsaturated fatty acids, but it lacks efficient g...
Yi Li,Jian-Qun Deng,An-Qi Wang et al.
Yi Li et al.
Heparin is a critically important clinical anticoagulant. The biosynthesis of heparin using Escherichia coli-based multienzyme cascades represents a promising alternative to animal-derived production. However, efficient deployment of hepari...
Pinak Samal,Roman Truckenmüller
Pinak Samal
Engineering the age(ing) of tissues in vitro could lead to more representative and predictive models for the ageing population. This forum introduces methodological approaches for 'age engineering' ('ageneering') and further discusses futur...
Engineered photothermal-responsive bacterial platform for in vivo fluorescence imaging-guided photothermal/gene combined breast cancer therapy [0.03%]
用于荧光影像引导的光热/基因联合乳腺癌治疗的工程光热响应细菌平台
Jingyu Zhang,Bin Guo,Zhenlong Wang et al.
Jingyu Zhang et al.
By leveraging the capacity of near-infrared light to penetrate deep tissue, we devised an engineered bacterial system for fluorescence imaging-guided photothermal/gene combined cancer therapy. By incorporating a thermosensitive plasmid carr...