Kaifang Liu,Zhilan Zhang,Guangjie Liang et al.
Kaifang Liu et al.
Microbial synthetic biology focuses on the application of rational engineering strategies to reprogram microbial cells, thereby providing them with novel functions to meet different requirements. However, this engineering process inherently...
Industrial perspective validation of a neutralizer-free l-lactic acid production process by engineered acid-tolerant yeast Pichia kudriavzevii [0.03%]
基于工程化耐酸假丝酵母的无中和剂L-乳酸生产工艺的工业视角验证
Yi Wang,Jiakai Cui,Zhe Liu et al.
Yi Wang et al.
Large amounts of waste salt formation are a major bottleneck in the industrial production of l-lactic acid (l-LA). In this study, we developed a cost-effective, neutralizer-free process with industrial potential using acid-tolerant yeast Pi...
Codon-optimized base editors enable efficient base substitution in Atlantic salmon [0.03%]
优化密码子的碱基编辑器能够高效地在大西洋鲑鱼中进行碱基替换
Jinhai Wang,Jiaqi Wang,Alexandra Florea et al.
Jinhai Wang et al.
Base editors (BEs) are promising gene-editing tools for correcting point mutations or generating new ones, broadly used in vitro and in model organisms, but their application in non-model animals remains limited. One of the major challenges...
Helena Codina,Marta Quero-Delgado,Rafael Gómez et al.
Helena Codina et al.
Dengue nanosensors promise much but have failed in clinical application. This paradox stems from a focus on record sensitivity over real-world robustness. We propose a paradigm shift from materials discovery to product engineering, providin...
Establishment of a nonhomologous end joining-mediated genomic library in Fusarium fujikuroi for high-level production of gibberellic acid [0.03%]
鬼伞菌中基于非同源末端连接的基因组文库的建立及其高产赤霉素品系的获得
Ya-Wen Li,Xin-Yu Chen,Hui-Zhi Lu et al.
Ya-Wen Li et al.
Gibberellic acid (GA3) is primarily produced by the liquid fermentation of Fusarium fujikuroi, yet its complex metabolic network limits rational strain engineering. Here, we strengthened the nonhomologous end joining (NHEJ) pathway and mapp...
Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis [0.03%]
用于增强微生物化学合成的基因组进化导向索引biosensor
Dianjiang Yu,Haofeng Chen,Yang Zhang et al.
Dianjiang Yu et al.
Microbial synthesis of chemicals still faces low production due to the complicated metabolic networks, which generally cannot be tackled by rational engineering strategies. In this study, we report on a workflow, biosensor-guided indexing o...
Roksolana Vasylyshyn,Justyna Ruchala,Kostyantyn Dmytruk et al.
Roksolana Vasylyshyn et al.
Most known yeasts are mesophilic organisms, with optimal growth at 28-30°C. However, a few species, notably Ogataea polymorpha and Kluyveromyces marxianus, can grow at temperatures around 50°C. Their thermotolerance is of both fundamental...
Trehalose 6-phosphate as master switch for optimizing crop yield and resilience [0.03%]
作为优化作物产量和恢复力的主调节因子的海藻糖-6-磷酸
Muhammad Waqas,Chen Yaning,Yong-Ling Ruan et al.
Muhammad Waqas et al.
Crop breeding faces the challenge of balancing yield with resource efficiency and stress tolerance. The key regulatory metabolite trehalose 6-phosphate (T6P) integrates carbon status with growth and stress responses. Manipulating T6P levels...
Legal consequences of breaching a European mandatory regulation on genome-edited crops [0.03%]
违反欧盟关于基因编辑作物强制性法规的法律后果
Lourdes Salomón,Teresa Capell-Capell,Paul Christou et al.
Lourdes Salomón et al.
Genome editing enables targeted mutations indistinguishable from those in conventional breeding. In December 2025, the EU adopted more permissive regulations for some genome-edited crops, in line with other jurisdictions. We discuss consequ...
Zhengshui Xu,Yu Li,Yundong Zhou et al.
Zhengshui Xu et al.
Chimeric antigen receptor natural killer (CAR-NK) cell therapy is an emerging cancer treatment offering advantages over CAR-T therapy, including reduced risks of cytokine release syndrome and graft-versus-host disease. This review highlight...