The genome editing revolution [0.03%]
John van der Oost,Constantinos Patinios
John van der Oost
Published Erratum
Trends in biotechnology. 2026 Apr 3:S0167-7799(26)00012-0. DOI:10.1016/j.tibtech.2026.01.012 2026
Recent advances in systems engineering approaches to integrated strain and bioprocess development [0.03%]
Jennifer N Hennigan,Maximillian Golovsky,Utsuki Yano et al.
Jennifer N Hennigan et al.
Systems engineering has transformed chemical manufacturing, but bioprocessing has lagged in adopting comprehensive approaches. This review explores strategies that successfully engineer integrated upstream and downstream bioprocesses. Our a...
Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions [0.03%]
Zhen Li,Xiaoling Wang,Jin Liu et al.
Zhen Li et al.
Adeno-associated viral (AAV) vectors are commonly used for genome editing owing to the proclivity with which their single-stranded genomes serve as homologous recombination (donor) substrates during programmable nuclease-assisted gene targe...
Sulfur assimilation determines S-adenosyl-l-methionine flux for enhancing methylation efficiency in heterologous biosynthesis [0.03%]
Xinchen Zhang,Ying Wang,Wenpei Huang et al.
Xinchen Zhang et al.
S-adenosyl-l-methionine (SAM) is not only crucial for cellular physiological processes as a methyl donor and signaling molecule but also a methyl donor for heterologous biosynthesis. Efforts for increasing SAM availability have focused prim...
Nil K Pandey,Hong Huy Tran,Edward B Steager et al.
Nil K Pandey et al.
Nanozymes (enzyme-like catalytic nanomaterials) represent a new generation of artificial enzymes designed to overcome key limitations of their natural counterparts, including high production costs, low stability, and challenging storage req...
Tarryn Lee Botha,Yu Shrike Zhang
Tarryn Lee Botha
Environmental toxicology relies on aquatic organisms to assess chemical safety, but ethical concerns are growing. We explore how bioprinting could be applied to aquatic models-from Daphnia analogs and bioprinted fish chorions to teleost-on-...
Zhiyi Lu,Zushuang Zhang,Peng Chen et al.
Zhiyi Lu et al.
Cofactor engineering is revolutionizing green biomanufacturing by overcoming the fundamental bottleneck between the limited supply/regeneration of cellular energy cofactors [e.g., NAD(P)H, ATP] and the high demands of efficient bioproductio...
Multigram-scale stereoselective synthesis of neurosteroid isomers by gut microbial isolates using plant biomass-derived medium [0.03%]
Ronnie G Gicana,Po-Hsiang Wang,Tien-Yu Wu et al.
Ronnie G Gicana et al.
We present a sustainable microbial platform utilizing gut bacteria and a plant-based medium for stereoselective neurosteroid biosynthesis. Through bioinformatics- and structural biology-guided screening of over 3000 bacterial isolates, we i...
Oded Ben-Joseph,Stanislav Glezer
Oded Ben-Joseph
Regulatory pathways strongly influence how drugs are developed, financed, and sold. This article compares the FDA's 505(b)(1) and 505(b)(2) routes, showing how the latter reduces both the development risk and the probability of postapproval...
Constança M Costa,Ana Paula Terrasso,Marta H G Costa et al.
Constança M Costa et al.
The clinical efficacy of cell therapy products is intrinsically related to their state of differentiation and maturity. However, current approaches to target cell phenotype and potency lack efficacy, scalability, and cost-effectiveness. Met...