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A modular all-in-one genetic engineering tool for the diatom Phaeodactylum tricornutum based on CRISPR-Cas12a

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The marine diatom Phaeodactylum tricornutum is a model microalgal species that is emerging as a platform for the photosynthetic production of industrial chemicals such as carotenoids and polyunsaturated fatty acids, but it lacks efficient genetic tools to real... ...