The regulation of the thermal stability and affinity of the HSPA5 (Grp78/BiP) by clients and nucleotides is modulated by domains coupling [0.03%]
客户蛋白和核苷酸通过结合域耦合调节HSPA5(Grp78/BiP)的热稳定性和亲和力
Noeli S M Silva,Bruna Siebeneichler,Carlos S Oliveira et al.
Noeli S M Silva et al.
The HSPA5 protein (BiP/Grp78) serves as a pivotal chaperone in maintaining cellular protein quality control. As a member of the human HSP70 family, HSPA5 comprises two distinct domains: a nucleotide-binding domain (NBD) and a peptide-bindin...
Co-chaperonin GroES subunit exchange as dependent on time, pH, protein concentration, and urea [0.03%]
协同分子GroES亚基交换取决于时间、PH值、蛋白质浓度和尿素的影响
Victor Marchenkov,Alexey Surin,Victor Ugarov et al.
Victor Marchenkov et al.
The discovery of a subunit exchange in some oligomeric proteins, implying short-term dissociation of their oligomeric structure, requires new insights into the role of the quaternary structure in oligomeric protein stability and function. H...
Mapping the Periostin splice isoforms in atopic dermatitis and an in vitro asthma model - A multi-platform analysis using mass spectrometry and RT-qPCR [0.03%]
基于质谱和RT-qPCR的多平台分析在特应性皮炎及体外哮喘模型中映射骨桥蛋白剪切异构体分布
Christian E Rusbjerg-Weberskov,Anne Kruse Hollensen,Christian Kroun Damgaard et al.
Christian E Rusbjerg-Weberskov et al.
Periostin is a matricellular protein known to be alternatively spliced to produce ten isoforms with a molecular weight of 78-91 kDa. Within the extracellular matrix, periostin attaches to cell surfaces to induce signaling via integrin-bindi...
Robust assessment of sample preparation protocols for proteomics of cells and tissues [0.03%]
用于细胞和组织蛋白质组学研究的样品前处理方案的效果评估
Francielle Aguiar Gomes,Douglas Ricardo Souza Junior,Mariana Pereira Massafera et al.
Francielle Aguiar Gomes et al.
In proteomic studies, the reliability and reproducibility of results hinge on well-executed protein extraction and digestion protocols. Here, we systematically compared three established digestion methods for macrophages, namely filter-assi...
Exploring liquid-liquid phase separation in the organisation of Golgi matrix proteins [0.03%]
液-液相分离在高尔基体矩阵蛋白组织中的作用探索
Luis Felipe S Mendes,Carolina G Oliveira,Kevin F Simões et al.
Luis Felipe S Mendes et al.
The Golgi apparatus is a critical organelle in protein sorting and lipid metabolism. Characterized by its stacked, flattened cisternal structure, the Golgi exhibits distinct polarity with its cis- and trans-faces orchestrating various prote...
Plumbagin accelerates serum albumin's amyloid aggregation kinetics and generates fibril polymorphism by inducing non-native β-sheet structures [0.03%]
白花丹素通过诱导非天然β折叠结构加速血清白蛋白淀粉样蛋白聚集并产生原纤维多态性
Chanchal Chauhan,Poonam Singh,Shivani A Muthu et al.
Chanchal Chauhan et al.
The ligand-induced conformational switch of proteins has great significance in understanding the biophysics and biochemistry of their self-assembly. In this work, we have investigated the ability of plumbagin (PL), a hydroxynaphthoquinone c...
Identification of the interfacial regions in misfolded transthyretin oligomers [0.03%]
鉴定误折叠的甲状腺转运蛋白低聚物的界面区域
Anvesh K R Dasari,Matthew F Coats,Abdullah B Ali et al.
Anvesh K R Dasari et al.
Misfolding and aggregation of transthyretin (TTR) is associated with numerous ATTR amyloidosis. TTR aggregates extracted from ATTR patients consist of not only full-length TTR, but also N-terminally truncated TTR fragments that can be produ...
Assessing the Martini 3 protein model: A review of its path and potential [0.03%]
评估Martini 3蛋白模型：回顾其路径和潜力
Luís Borges-Araújo,Gilberto P Pereira,Mariana Valério et al.
Luís Borges-Araújo et al.
Coarse-grained (CG) protein models have become indispensable tools for studying many biological protein details, from conformational dynamics to the organization of protein macro-complexes, and even the interaction of proteins with other mo...
The molecular interplay between human and bacterial amyloids: Implications in neurodegenerative diseases [0.03%]
人和细菌淀粉样蛋白之间的分子相互作用：在神经退行性疾病中的影响
Neha Jain
Neha Jain
Neurodegenerative disorders such as Parkinson's (PD) and Alzheimer's diseases (AD) are linked with the assembly and accumulation of proteins into structured scaffold called amyloids. These diseases pose significant challenges due to their c...
Chemical proteomics approaches for protein post-translational modification studies [0.03%]
化学蛋白质组学方法用于蛋白质翻译后修饰研究
Nan Zhang,Jinghua Wu,Qingfei Zheng
Nan Zhang
The diversity and dynamics of proteins play essential roles in maintaining the basic constructions and functions of cells. The abundance of functional proteins is regulated by the transcription and translation processes, while the alternati...