Replacement of the essential catalytic aspartate with serine leads to an active form of copper-containing nitrite reductase from the denitrifier Sinorhizobium meliloti 2011 [0.03%]
天冬氨酸变丝氨酸后生成的铜含硝基还原酶为有效形式：来自土壤固氮菌Sinorhizobium meliloti 2011的铜含硝基还原酶
Lorieth A Guevara Cuasapud,Pablo J González,Félix M Ferroni et al.
Lorieth A Guevara Cuasapud et al.
We report the molecular, biochemical and spectroscopic characterization and computational calculations of a variant of the copper-containing nitrite reductase from the rhizobial microorganism S. meliloti (SmNirK), in which the catalytic asp...
Mikhail Matveyenka,Abid Ali,Charles L Mitchell et al.
Mikhail Matveyenka et al.
Progressive aggregation of α-synuclein (α-Syn), a small cytosolic protein involved in cell vesicle trafficking, in the midbrain, hypothalamus, and thalamus is linked to Parkinson's disease (PD). Amyloid oligomers and fibrils formed as a r...
Unlocking the wound-healing potential: An integrative in silico proteomics and in vivo analysis of Tacorin, a bioactive protein fraction from Ananas comosus (L.) Merr. Stem [0.03%]
解密Tacorin的促伤口愈合潜能：一种菠萝冠果茎中的生物活性蛋白质组学与体内分析整合研究
Puji Rahayu,Doni Dermawan,Florensia Nailufar et al.
Puji Rahayu et al.
Tacorin, a bioactive protein fraction derived from pineapple stem (Ananas comosus), has emerged as a promising therapeutic agent for wound healing. This study employs an integrated approach, combining in silico proteomics and in vivo invest...
A distinct co-expressed sulfurtransferase extends the physiological role of mercaptopropionate dioxygenase in Pseudomonas aeruginosa PAO1 [0.03%]
一种独特的共表达硫转移酶扩展了绿脓杆菌PAO1中巯基丙酸双加氧酶的生理作用
Chukwuemeka S Adindu,Katie Tombrello,Luke A Martz et al.
Chukwuemeka S Adindu et al.
Oxidation and assimilation of persulfides in bacteria is often catalyzed by a persulfide dioxygenase and sulfurtransferase in consecutive reactions. Enzymes responsible for the oxidation of persulfides have not been clearly defined in Pseud...
Deciphering the cleavage sites of 3C-like protease in Gammacoronaviruses and Deltacoronaviruses [0.03%]
解读冠状病毒属γ亚属和δ亚属的3CL蛋白酶切割位点
Mengxue Wang,Xinyi Sun,Shijiang Peng et al.
Mengxue Wang et al.
Coronaviruses replicate by using the 3C-like protease (3CLpro) to cleave polyprotein precursors and host proteins. However, current tools for identifying 3CLpro cleavage sites are limited, particularly in Gammacoronaviruses (GammaCoV) and D...
CDR identification, epitope mapping and binding affinity determination of novel monoclonal antibodies generated against human apolipoprotein B-100 [0.03%]
人载脂蛋白B-100单克隆抗体的鉴定、表位图谱绘制及亲和力测定
Tariga Sritrakarn,Kanokwan Lowhalidanon,Panida Khunkaewla
Tariga Sritrakarn
In-house generated mAbs to apolipoprotein B-100 (apoB-100) clones hLDL-E8, hLDL-2D8 and hLDL-F5 were extensively studied to determine their complementarity-determining regions (CDRs), binding epitopes and affinity. RT-PCR revealed that all ...
The role of proton-coupled electron transfer from protein to heme in dehaloperoxidase [0.03%]
质蛋白偶联电子转移在脱卤过氧化物酶中的作用
Mst Sharmin Aktar,Nikhila Kashyap Dhanvantari Madhuresh,Reza A Ghiladi et al.
Mst Sharmin Aktar et al.
At least two of the six methionine (Met) residues in dehaloperoxidase (DHP) are shown to act as electron donors in both autoreduction and protein-heme crosslinking. Autoreduction observed in the two isozymes, DHP-A and DHP-B, is explained b...
Incorporation of pyridoxal-5'-phosphate into the apoenzyme: A structural study of D-amino acid transaminase from Haliscomenobacter hydrossis [0.03%]
霍氏球鼓藻细菌D-氨基酸转氨酶的PLP催化的结构研究
Alina K Bakunova,Ilya O Matyuta,Mikhail E Minyaev et al.
Alina K Bakunova et al.
Pyridoxal-5'-phosphate (PLP)-dependent transaminases are key enzymes of amino acid metabolism in cells and remarkable biocatalysts of stereoselective amination for process chemistry applications. As cofactor-dependent enzymes, transaminases...
Ndufs4 knockout mice with isolated complex I deficiency engage a futile adaptive brain response [0.03%]
Ndufs4敲除小鼠的孤立复合物I缺乏激活徒劳的适应性脑反应
Melissa A E van de Wal,Cenna Doornbos,Janne M Bibbe et al.
Melissa A E van de Wal et al.
Paediatric Leigh syndrome (LS) is an early-onset and fatal neurodegenerative disorder lacking treatment options. LS is frequently caused by mutations in the NDUFS4 gene, encoding an accessory subunit of mitochondrial complex I (CI), the fir...
Phosphoproteome modulation by nucleoside diphosphate kinase affects photosynthesis & stress tolerance of Nostoc PCC 7120 [0.03%]
核苷二磷酸激酶通过调节磷酸化蛋白质组影响鱼腥藻7120的光合作用和胁迫耐受性
Anurag Kirti,Hema Rajaram
Anurag Kirti
Nucleoside diphosphate kinase (Ndk/NDK/NDPK) is known to possess pleiotropic functions, one of which is that as a protein kinase, and has been shown to be involved in stress tolerance in plants. To assess its role in the cyanobacterium Nost...