Efficient production of a novel recombinant fusion protein of EIEC effector IpaD and EGFP: Biophysical characterization and functional studies [0.03%]
产志贺毒素大肠杆菌效应蛋白IpaD与增强型绿色荧光蛋白融合表达的生物物理特性及功能研究
Sudeshna Halder,Namita Jaiswal,Salari Charan Balajee et al.
Sudeshna Halder et al.
The conserved invasion plasmid antigen D (IpaD) protein demonstrates broad protective capabilities against bacillary dysentery caused by Enteroinvasive Escherichia coli (EIEC) and Shigella. However, the instability of the IpaD protein at ro...
A Bartkowiak,Ewa Szczesny-Malysiak,Jakub Dybas
A Bartkowiak
Heme proteins are a large group of biomolecules with heme incorporated as a prosthetic group. Apart from cytochromes present in almost all cell types, many other specific heme proteins are expressed in different kinds of cells, e.g. hemoglo...
DSP-1, the major fibronectin type-II protein of donkey seminal plasma is a small heat-shock protein and exhibits chaperone-like activity against thermal and oxidative stress [0.03%]
DSP-1是公驴精液中主要的纤维连接蛋白II型蛋白，是一种小热休克蛋白，并对热和氧化应激表现出分子伴侣活性。
Sk Alim,Sudheer K Cheppali,Sonali S Pawar et al.
Sk Alim et al.
Fibronectin type-II (FnII) proteins are major constituents in the seminal plasma of many mammals and play a crucial role in sperm capacitation. Additionally, the seminal FnII proteins from bull and horse exhibit chaperone-like activity (CLA...
Participation of a cysteine tetrad in the recycling mechanism of methionine sulfoxide reductase a from radiation-tolerant Deinococcus bacteria [0.03%]
参与放线菌素耐受性德尼伯克氏菌甲硫氨酸硫酸盐还原酶A的循环机制中的半胱氨酸四联体的作用
Pascal Rey,Nicolas Rouhier,Chloé Carassus et al.
Pascal Rey et al.
Methionine oxidation leads to the formation of methionine sulfoxide (MetO), which is reduced back to Met by methionine sulfoxide reductases (Msrs). The catalytic mechanism used by A-type Msr (MsrA) for MetO reduction requires a catalytic cy...
Replacement of the essential catalytic aspartate with serine leads to an active form of copper-containing nitrite reductase from the denitrifier Sinorhizobium meliloti 2011 [0.03%]
天冬氨酸变丝氨酸后生成的铜含硝基还原酶为有效形式：来自土壤固氮菌Sinorhizobium meliloti 2011的铜含硝基还原酶
Lorieth A Guevara Cuasapud,Pablo J González,Félix M Ferroni et al.
Lorieth A Guevara Cuasapud et al.
We report the molecular, biochemical and spectroscopic characterization and computational calculations of a variant of the copper-containing nitrite reductase from the rhizobial microorganism S. meliloti (SmNirK), in which the catalytic asp...
Mikhail Matveyenka,Abid Ali,Charles L Mitchell et al.
Mikhail Matveyenka et al.
Progressive aggregation of α-synuclein (α-Syn), a small cytosolic protein involved in cell vesicle trafficking, in the midbrain, hypothalamus, and thalamus is linked to Parkinson's disease (PD). Amyloid oligomers and fibrils formed as a r...
Unlocking the wound-healing potential: An integrative in silico proteomics and in vivo analysis of Tacorin, a bioactive protein fraction from Ananas comosus (L.) Merr. Stem [0.03%]
解密Tacorin的促伤口愈合潜能：一种菠萝冠果茎中的生物活性蛋白质组学与体内分析整合研究
Puji Rahayu,Doni Dermawan,Florensia Nailufar et al.
Puji Rahayu et al.
Tacorin, a bioactive protein fraction derived from pineapple stem (Ananas comosus), has emerged as a promising therapeutic agent for wound healing. This study employs an integrated approach, combining in silico proteomics and in vivo invest...
A distinct co-expressed sulfurtransferase extends the physiological role of mercaptopropionate dioxygenase in Pseudomonas aeruginosa PAO1 [0.03%]
一种独特的共表达硫转移酶扩展了绿脓杆菌PAO1中巯基丙酸双加氧酶的生理作用
Chukwuemeka S Adindu,Katie Tombrello,Luke A Martz et al.
Chukwuemeka S Adindu et al.
Oxidation and assimilation of persulfides in bacteria is often catalyzed by a persulfide dioxygenase and sulfurtransferase in consecutive reactions. Enzymes responsible for the oxidation of persulfides have not been clearly defined in Pseud...
Deciphering the cleavage sites of 3C-like protease in Gammacoronaviruses and Deltacoronaviruses [0.03%]
解读冠状病毒属γ亚属和δ亚属的3CL蛋白酶切割位点
Mengxue Wang,Xinyi Sun,Shijiang Peng et al.
Mengxue Wang et al.
Coronaviruses replicate by using the 3C-like protease (3CLpro) to cleave polyprotein precursors and host proteins. However, current tools for identifying 3CLpro cleavage sites are limited, particularly in Gammacoronaviruses (GammaCoV) and D...
CDR identification, epitope mapping and binding affinity determination of novel monoclonal antibodies generated against human apolipoprotein B-100 [0.03%]
人载脂蛋白B-100单克隆抗体的鉴定、表位图谱绘制及亲和力测定
Tariga Sritrakarn,Kanokwan Lowhalidanon,Panida Khunkaewla
Tariga Sritrakarn
In-house generated mAbs to apolipoprotein B-100 (apoB-100) clones hLDL-E8, hLDL-2D8 and hLDL-F5 were extensively studied to determine their complementarity-determining regions (CDRs), binding epitopes and affinity. RT-PCR revealed that all ...