Disconnect between COX-2 selective inhibition and cardiovascular risk in preclinical models [0.03%]
关于选择性COX-2抑制与心血管风险的前临床模型之间的差异分析
Yevgeniya E Koshman,Aimee L Bielinski,Brandan M Bird et al.
Yevgeniya E Koshman et al.
Introduction: Secondary pharmacology profiling is routinely applied in pharmaceutical drug discovery to investigate the pharmaceutical effects of a drug at molecular targets distinct from (off-target) the intended therape...
Randomized Controlled Trial
Journal of pharmacological and toxicological methods. 2023 Mar-Apr:120:107251. DOI:10.1016/j.vascn.2023.107251 2023
Revisiting the high-fat diet/low streptozotocin prediabetic rat model: A bioanalytical adjustment [0.03%]
重新审视高脂饮食/低剂量链脲佐菌素诱导的糖尿病前期大鼠模型：生物分析调整
Alejandra M Preciado-Saldaña,José A López-Díaz,J Abraham Domínguez-Avila et al.
Alejandra M Preciado-Saldaña et al.
Insulin resistance (IR) is the main feature of prediabetes (PD), which ultimately leads to diabetes. High-dose streptozotocin-treated rodents often show irreversible β-cell mass loss and function, leaving the premorbid diabetic state (PD/I...
Simultaneous quantification of thalidomide, lenalidomide and pomadomide in plasma by LC-MS/MS [0.03%]
使用LC-MS / MS同时量化血浆中的沙利度胺，来那度胺和泊马度胺
Bin Wang,Wanting Qiang,Jia Yi et al.
Bin Wang et al.
Objective: To develop a new method for quantitatively analyzing three immunomodulators (thalidomide, lenalidomide and pomadomide) by liquid chromatography tandem mass spectrometry (LC-MS/MS). ...
Reference intervals and method sensitivity for electrocardiology, hemodynamics, and body temperature parameters in healthy cynomolgus monkeys [0.03%]
健康普通猕猴心电图、血流动力学和体温参数的参考区间及方法灵敏度研究
Xiefan Fang,Stephen D Tichenor
Xiefan Fang
In nonclinical studies, electrocardiograms (ECG) of cynomolgus monkey are recorded intermittently by external leads in manually restrained animals (snapshot recording) or continuously by jacketed external telemetry (JET) or implanted radiot...
An electrochemiluminescence assay for quantification of Denileukin Diftitox and its anti-drug antibodies in rat serum [0.03%]
用于定量检测大鼠血清中Denileukin Diftitox及其抗药抗体的电化学发光测定法
Yuji Mano
Yuji Mano
Denileukin Diftitox (DD), comprising fragments of diphtheria toxin (DT) and interleukin-2 (IL2), was developed for the treatment of lymphoma and has been approved for marketing in Japan. Toxicological evaluation including pharmacokinetics a...
Non-uniformity in in vitro drug-induced cytotoxicity as evidenced by differences in IC50 values - implications and way forward [0.03%]
药物体外诱导细胞毒性的不均一性--IC50值差异所反映的问题及对策
T Arokia Femina,V Barghavi,K Archana et al.
T Arokia Femina et al.
Cell lines have proven indispensable for in vitro experiments and their utility as experimental models range from understanding the fundamental cell functioning to drug discovery. One of the most common utility of cell lines is for in vitro...
Protein malnutrition in BALB/C mice: A model mimicking clinical scenario of marasmic-kwashiorkor malnutrition [0.03%]
BALB/C小鼠的蛋白质营养不良：一种模仿临床干瘦型-水肿型营养不良状况的模型
Madhura R J,Varsha A,Anirban Chakraborthy et al.
Madhura R J et al.
Protein malnutrition continues to be a major global issue. A stable animal model to address protein malnutrition and its effect on various disease conditions is necessary. In the present study, we have formulated and standardized a low prot...
Determination of five positive control drugs in hERG external solution (buffer) by LC-MS/MS to support in vitro hERG assay as recommended by ICH S7B [0.03%]
采用LC-MS/MS测定hERG体外实验阳性质控药物支持ICH S7B建议的hERG检测
Tamara I King,Amruta Indapurkar,Isra Tariq et al.
Tamara I King et al.
ICH S7B recommends screening for hERG channel block using patch clamp recordings to assess a drug's proarrhythmic risk. Block of the hERG channel has been associated with clinical QTC prolongation as well as the rare, but potentially fatal ...
Yi Zeng,Huidong Jin,Jia Wang et al.
Yi Zeng et al.
Non-invasive intratracheal instillation is an important method for direct exposure of the respiratory tract which is commonly used in toxicology, environmental science, and other research fields. However, there is no standard operating proc...
Importance of beating rate control for the analysis of drug effects on contractility in human induced pluripotent stem cell-derived cardiomyocytes [0.03%]
探讨药物对人类诱导多能干细胞源心肌细胞收缩力影响时控制搏动速率的重要性分析
Yuto Hinata,Yuki Kagawa,Hirotsugu Kubo et al.
Yuto Hinata et al.
Cardiac contractility evaluation using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has recently attracted much attention as a clinical cardiotoxicity predictive model. Most studies on this were conducted under spo...