A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its "cell-like" immobilized form [0.03%]
一种利用枯草芽孢杆菌硝基还原酶及其“细胞类似”固定化形式降解硝基芳烃的简便高效途径
Ze Li,Ziyi Yang,Liangyu Zheng
Ze Li
Nitroaromatic compounds are persistent environmental pollutants that pose significant ecological and toxicological risks. Enzymatic reduction of nitroaromatics to the corresponding aromatic amines offers an efficient and selective degradati...
Direct recovery of free astaxanthin from Haematococcus pluvialis: Bifunctional deep eutectic solvent-mediated extraction and conversion in a single-step [0.03%]
一种从雨生红球藻中直接提取虾青素的新方法：单步构建双功能深共晶溶剂同步萃取及转化
Shumiao Chen,Zetao Shen,Lijiao Wen et al.
Shumiao Chen et al.
In this work, a series of deep eutectic solvents (DESs) were designed, using diamines as hydrogen bond acceptors (HBAs) and phenol derivatives as hydrogen bond donors (HBDs). The DES composed of 1,3-propanediamine and m-cresol was screened ...
Efficient expression of γ-DL-glutamyl hydrolase in Komagataella phaffii and its application in modification of poly-γ-glutamic acid from Bacillus velezensis [0.03%]
毕赤酵母高效表达谷氨酰胺转肽酶及其在伏击芽孢杆菌聚 γ- 谷氨酸修饰中的应用
Hong Liu,Qiaojuan Yan,Yanxiao Li et al.
Hong Liu et al.
The functional properties and application performance of poly-γ-glutamic acid (γ-PGA) are strongly dependent on its molecular weight (MW). However, the precise control of MW remains challenging, significantly hindering its high-value appl...
Engineering AraC L9K for inducer-independent pBAD activation in Salmonella Gallinarum [0.03%]
工程改造沙门氏菌Gallinarum中的AraC L9K以实现诱导剂独立的pBAD激活功能
Taner Duysak
Taner Duysak
The AraC-pBAD promoter system is widely used for tunable gene expression in bacteria, but its dependence on arabinose as an inducer limits industrial scalability and complicates therapeutic deployment. In this study, an inducer-independent ...
Pathway optimization for effective heterologous synthesis of ergothioneine in Aspergillus niger [0.03%]
一种优化异源合成麦角硫因的路径及在黑曲霉中高效表达的方法
Lihong Chen,Qi Liu,Li Pan et al.
Lihong Chen et al.
Ergothioneine (EGT) is a potent natural antioxidant, making it a promising candidate for potential applications in cosmetics, pharmaceuticals, and food industries. This study represents the successful heterologous synthesis of EGT in Asperg...
Tailored base editing toolkits for functional genomics and metabolic engineering in the halophile Salinivibrio [0.03%]
嗜盐菌盐单胞菌功能基因组学和代谢工程的定制碱基编辑工具包
Meng-Ru Wang,Jiujiu Yi,Zheng-Jun Li
Meng-Ru Wang
The development of synthetic biology in non-model halophiles has been hindered by the lack of efficient genetic tools, limiting their industrial applications. In this study, we report the development of an efficient base editing platform fo...
Algae-based biomaterial synthesis and applications for a sustainable green bioeconomy: A review [0.03%]
基于藻类的生物材料合成及在可持续绿色生物经济中的应用：综述研究
Chengxiao Ma,Xian Zhou,Bochen Zhao et al.
Chengxiao Ma et al.
The development of biological materials to reduce the dependence on non-renewable resources receives increasing interest. Algae are photoautotrophs, have extremely low growth requirements, can be easily cultivated and are very suitable for ...
Hydrogen production by immobilised Rhodopseudomonas palustris in two photobioreactor configurations [0.03%]
固定化Rhodopseudomonas palustris在两种光生物反应器配置下产生氢气
Grant Keet,Robert W M Pott
Grant Keet
In this study, the phototrophic bacterium, Rhodopseudomonas palustris was immobilised at differing cell densities in transparent, chemically and mechanically resistant, biocompatible polyvinyl alcohol-based hydrogels and employed in photobi...
Multimetallic biochar as an ecosystem engineer: Orchestrating synergistic IHT-DIET pathways via spatial niche partitioning for enhanced anaerobic digestion [0.03%]
多金属生物炭作为生态系统工程师：通过空间生态位分化调控协同内部/外部热解-饮食路径以强化厌氧消化性能
Yuanfang Deng,Xiaofan Yang,Danyang Shi et al.
Yuanfang Deng et al.
The efficacy of anaerobic digestion (AD) is limited by inefficient interspecies electron flow and metabolic pathways. While conductive materials promote direct interspecies electron transfer (DIET), their potential to engineer microbial eco...
Comparative analysis of manufacturability and binding of various bispecific antibody architectures [0.03%]
几种双特异性抗体架构的可制造性及结合特性比较分析
Juan Carlos Rivera-Castro,Octavio T Ramírez,Laura A Palomares
Juan Carlos Rivera-Castro
Bispecific monoclonal antibodies (BsAbs) enable novel therapeutic mechanisms with multiple binding specificities, but their architecture can influence manufacturability and function. We compared the production of five formats of a BsAb reco...