Newly isolated Corynebacterium glutamicum strain PP80 for use in high-temperature L-glutamic acid fermentation [0.03%]
用于高温L-谷氨酸发酵的新分离棒状杆菌菌株PP80
Naoya Kataoka,Ryo Koga,Nawarat Nantapong et al.
Naoya Kataoka et al.
High-temperature fermentation is a strategy to increase the fermentation rates and process stability while reducing cooling costs; however, metabolic heat generated during large-scale cultivations can impair cell growth and product formatio...
Fabrication of covalent immobilizers utilizing chitosan-magnesium chelating interactions [0.03%]
利用壳聚糖-镁螯合作用制备共价固定化基质
Marwa I Wahba
Marwa I Wahba
The chelating interactions amongst chitosan (CS) and Mg²⁺ were exploited to fabricate CS beads, with glutaraldehyde (GA) incorporated into the gelling solution to introduce the functionalities necessary for β-galactosidase (βGAL) covale...
New design of a chimeric enzyme based on vanadium-dependent bromoperoxidase and glucose oxidase to highlight the microbicidal properties of the reaction products: HOBr and HOCl [0.03%]
基于溴化过氧化物酶和葡萄糖氧化酶的嵌合型钒依赖酶的新设计：强调反应产物HOBr和HOCl的杀菌特性
Valentin Amalric,Sébastien Gounel,Aurélien Thureau et al.
Valentin Amalric et al.
Vanadium-dependent haloperoxidases are enzymes found in bacteria, fungi and red or brown macroalgae. They are used as a defense system for the latter by producing bromoform. The intermediate product, HOBr or HOCl, is also a microbicidal com...
A genome-wide coverage-based pipeline for the identification of host-derived candidate biomarkers in blood cell-free DNA [0.03%]
一种基于基因组广覆盖的血浆细胞游离DNA鉴定宿主候选生物标志物的方案
Alessandra Vittorini Orgeas,Christoph W Sensen
Alessandra Vittorini Orgeas
We have created a new data-analysis pipeline for the discovery of host-derived candidate biomarkers in blood cell-free DNA sequencing data. Unlike approaches that rely on specific molecular or genetic signatures, our method leverages the co...
Preparation of different immobilized/stabilized biocatalyst of Alcalase by modification with glutaraldehyde [0.03%]
戊二醛改性制备不同形式的Alcalase固定化/稳定化酶制剂
Thiago Queiroz Jardim Rodrigues,Vinicius Zimmermann,Pedro Abellanas-Perez et al.
Thiago Queiroz Jardim Rodrigues et al.
In this work, different strategies for the stabilization of commercial Alcalase based on glutaraldehyde modification were investigated. First, the free Alcalase was modified with 0.01, 0.1, or 1% (v/v) glutaraldehyde, which led to a signifi...
Beyond Chitin: Three Microbial Production Architectures for Landfill-Degradable Commodity Plastics [0.03%]
超越几丁质：用于制造可降解塑料的三种微生物生产架构
Christopher Childers
Christopher Childers
The bioplastics field has oriented around industrial compostability as the primary biodegradation target. We argue this constitutes a regime mismatch: most plastic waste enters landfills - anaerobic, ambient-temperature environments in whic...
Increased tolerance to biomass-derived inhibitors by deletion of the transcriptional regulator mraZ in Cupriavidus necator H16 [0.03%]
通过删除转录调节因子mraZ提高Cupriavidus necator H16对生物量衍生抑制剂的耐受性
Taeyun Kim,Yuni Shin,Geonwoo Doh et al.
Taeyun Kim et al.
Lignocellulosic biomass is an attractive renewable feedstock for sustainable biomanufacturing, but inhibitors generated during pretreatment and saccharification severely limit microbial growth and productivity. Among various strategies to o...
Engineering and diverse biomedical applications of bacteriophytochromes [0.03%]
工程与多样化的生物医学应用细菌光敏色素研究
Xiaoxue Han,Yuhan Lv,Suna Qian et al.
Xiaoxue Han et al.
Bacteriophytochromes (BphPs) are a class of widely distributed biliverdin (BV) bound bacterial photoreceptor proteins that can respond to red or far-red light. BphPs have been employed as photoresponsive module in red/far-red light control ...
Reprogrammed Komagataella phaffii for enhanced secretory expression of human lactoferrin [0.03%]
用于增强人乳铁蛋白分泌表达的重组Komagataella phaffii细胞系
Kun Liu,Xinlan Fan,Zhen Tong et al.
Kun Liu et al.
Human lactoferrin (hLF) is a multifunctional glycoprotein of the transferrin family derived from milk and mucosal secretions, which exhibits antibacterial, anti-tumor, and immunomodulatory functions, and is an important component of infant ...
Efficient rDNA-mediated multi-copy integration of gene clusters in Aureobasidium melanogenum [0.03%]
高效的rDNA介导的基因簇多拷贝整合应用于 Aureobasidium melanogenum
Xinxin Kang,Yixin Wu,Jia Xu et al.
Xinxin Kang et al.
Aureobasidium melanogenum is a promising non-conventional yeast chassis for synthetic biology. However, techniques recombining large genetic fragments, such as gene clusters, are still unavailable, hindering further metabolic reprogramming ...