Beyond tyrosine feeding: A novel fed-batch cultivation strategy based on tyrosine metabolic engineering in recombinant CHO cells [0.03%]
脯氨酸分批补料灌流培养生产重组CHO细胞系中的蛋白质药物的新策略
Lei Cao,Liang Zhao,Qian Ye et al.
Lei Cao et al.
CHO cells dominate monoclonal antibody (mAb) production in fed-batch biomanufacturing, where tyrosine supply is limited by low solubility in neutral media and the complexities of alkaline tyrosine feeds. Existing studies confirm overexpress...
Efficient CRISPR/Cas-based gene editing in cotton induced by cotton leaf crumple virus [0.03%]
棉花叶片卷曲病毒诱导的棉花CRISPR / Cas基因组编辑效率低
Jiajun Zhang,Peihong Dai,Zheng Weng et al.
Jiajun Zhang et al.
Plant viral vectors can replicate autonomously and spread within host cells, making them an ideal tool for the delivery of CRISPR/Cas gene-editing elements. Here, we constructed a cotton CRISPR/Cas system mediated by cotton leaf crumple vir...
Enhanced interfacial microbial degradation of n-hexane-contaminated waste gas using a novel magnetic silicone oil [0.03%]
一种新型磁性硅油增强界面微生物降解正己烷废气研究
Guangquan Ou,Fangzhen Ran,Zhengwei Shang et al.
Guangquan Ou et al.
Two-phase partitioning bioreactors have addressed the challenges of treating waste gases contaminated with hydrophobic volatile organic compounds (VOCs), primarily by improving mass transfer. However, the efficient recovery of the non-aqueo...
Modular Engineering of the Doxorubicin Biosynthetic Gene Cluster Based on Component Functional Clustering in Streptomyces [0.03%]
基于链霉菌多组分功能聚类的多柔比星生物合成基因簇模块化工程
Tianqi Cui,Chanjuan Jiang,Jiale Li et al.
Tianqi Cui et al.
The anticancer drug doxorubicin is synthesized through the synergistic action of the type II polyketide synthases, along with an array of modification enzymes in Streptomyces. The 33 doxorubicin biosynthetic genes are scattered across at le...
Enhancement of natamycin production in Streptomyces gilvosporeus through heavy ion beam mutagenesis and global transcription machinery engineering [0.03%]
通过重离子束诱变和全转录调控装置工程化技术提高Streptomyces gilvosporeus中纳他霉素的产量
Yuxiu Xu,Liang Wang,Wei Hu et al.
Yuxiu Xu et al.
Natamycin is an antifungal agent produced by Streptomyces and is widely used in food, pharmaceuticals, and other fields. However, the low production of natamycin limits its application in various fields, making it crucial to enhance the bio...
Optimizing Chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortia [0.03%]
利用微藻-固氮细菌联合作用优化小球藻生产及其对种子萌发的影响研究
Ana Sanchez-Zurano,Silvia Vilaró-Cos,Daniel Figueiredo et al.
Ana Sanchez-Zurano et al.
Microalgal biomass is increasingly valued in industrial and agricultural sectors due to its bioactive compounds. However, large-scale production remains costly, mainly due to nitrogen fertilizer expenses. A promising sustainable alternative...
Komagataella phaffii as a microbial cell factory for antimicrobial peptide production [0.03%]
毕赤酵母生产抗菌肽的研究进展
Michel Lopes Leite,Kamila Botelho Sampaio de Oliveira,Letícia Ferreira Lima et al.
Michel Lopes Leite et al.
In recent decades, the methylotrophic yeast Komagataella phaffii has emerged as a powerful host for the heterologous production of antimicrobial peptides (AMPs) as an economical and scalable platform. K. phaffii combines several important a...
High-concentration Mg2+ stress improves L-lactic acid biosynthesis of Bacillus coagulans revealed by combined analysis of transcriptome and metabolome [0.03%]
结合转录组和代谢组分析揭示高浓度Mg2+胁迫提高凝结芽孢杆菌L-乳酸生物合成
Zhitong Sun,Jiaxin Liang,Gang Wang et al.
Zhitong Sun et al.
The application of magnesium oxide as a neutralizing agent significantly enhanced L-lactic acid (L-LA) production in Bacillus coagulans (B. coagulans), increasing titer, yield, productivity, and cell viability by 21.81%, 7.61%, 22.22%, and ...
Chitosan application in cosmetics and dermatology - antimicrobial and prebiotic potential to control human microbiome [0.03%]
壳多糖在化妆品和皮肤病学中的应用-调控人体微生物组的抗菌和益生潜力
Masha Delic,Irina Butorova,Andrey Kuskov
Masha Delic
The human microbiome has become a critically important field of study, with recent advances continuously revealing new aspects of its functional roles. A deeper understanding of the complex metabolic interactions between the microbiome, the...
Seaweed-derived biostimulants for sustainable crop production: A review [0.03%]
海藻源生物刺激素在作物可持续生产中的作用：综述
Mohamed Lamine Rabhi,Larbi Derbak,Hamdi Bendif et al.
Mohamed Lamine Rabhi et al.
The increasing concerns over climate changes and environmental impacts of synthetic agrochemicals have led to a growing interest in natural alternatives such as seaweed-based biostimulants. These extracts contain diverse bioactive compounds...