Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders [0.03%]
一种用于选择性量化复合益生菌粉中活的罗伊氏乳杆菌的矩阵匹配校正的单氮渍茋𬭩盐单azide-qPCR检测方法的建立和验证
Jinho Lee,Jin Seok Moon
Jinho Lee
Accurate enumeration of viable probiotic cells in commercial products is essential for quality control, label compliance, and product development. Conventional plate counting is labour-intensive and primarily quantifies culturable cells, wh...
Afonso Mota,Alessa Schiele,Isabel Santos de Sousa et al.
Afonso Mota et al.
Reproducibility and standardization of methods are key approaches to microbiological research. However, laboratories differ widely in equipment, protocols, and operator expertise. These disparities are especially pronounced in fungal biolog...
Detection of Listeria monocytogenes using anti-internalin antibodies generated through epitope prediction [0.03%]
通过表位预测生成抗内部蛋白抗体检测单核细胞增生李斯特菌
Mariya Divanshi,Jitesh Tarak,Shreya Saha et al.
Mariya Divanshi et al.
Listeria monocytogenes is a major food borne pathogen, and rapid, highly specific immunodetection tools are essential for surveillance in dairy food systems. This study reports an epitope-guided strategy for developing anti-Internalin A (In...
MobiRes: An integrative pipeline for resistome risk prediction through mobilome profiling [0.03%]
MobiRes：通过可移动基因组谱系进行抗性基因组风险预测的集成管道
Santhiya Kalimuthu,Ananthasubramanian Muthusamy
Santhiya Kalimuthu
Antimicrobial resistance (AMR) poses a significant global health challenge, with the environment serving as a crucial reservoir and conduit for resistance determinants. Although antibiotic resistance genes (ARGs) have been extensively studi...
Development and evaluation of a PMA-ddPCR for accurate detection of viable hypervirulent Klebsiella pneumoniae [0.03%]
一种用于准确检测高毒力肺炎克雷伯菌活菌的PMA-ddPCR方法的建立及评价
Zhiyu Li,Minjie Yang,Hongwen Duan et al.
Zhiyu Li et al.
Background: The global prevalence of hypervirulent Klebsiella pneumoniae (hvKP) infection is rising. Its high virulence, transmissibility, and propensity to enter the viable but non-culturable (VBNC) state compromise the ...
TSA-ultrasound synergy enhances CRISPR-Cas9 gene editing efficiency in diploid yeast [0.03%]
TSA和超声协同增强双倍体酵母CRISPR-Cas基因编辑效率
Ge Huang,Bin Zhuge,Xueqing Du et al.
Ge Huang et al.
The CRISPR-Cas9 system is invaluable for microbial engineering. However, its efficiency remains limited in numerous microorganisms, especially in polyploid yeasts where the compact chromatin structures pose significant physical barriers, wh...
Hadi H Mohammad,Media K Ismail
Hadi H Mohammad
Recent advances in peptide inhibitors have highlighted their therapeutic potential across cancers, neurodegenerative, cardiovascular, and metabolic diseases, driving the need for efficient molecular genetic methods to generate large peptide...
Technical benchmarking of library preparation kits for accurate WGS of picogram-level Mycobacterium tuberculosis DNA without whole-genome amplification [0.03%]
无需全基因组扩增的微量结核分枝杆菌DNA精确全基因组测序文库构建套件的技术评估
P Siyengu,J Loubser,R M Warren et al.
P Siyengu et al.
Whole‑genome sequencing of GC rich Mycobacterium tuberculosis is constrained by low DNA input. We compared two standard and two specialised low‑input library preparation kits using picogram‑level DNA without whole‑genome amplification. ...
Production optimization and characterization of amylase from Bacillus subtilis strain 2i under submerged fermentation [0.03%]
芽孢杆菌2i产淀粉酶的发酵优化及酶学性质研究
Maryam Kainat,Dian Nugraheni,Titin Haryati et al.
Maryam Kainat et al.
Amylase holds significant importance in industrial biotechnology. In this study, amylase production by Bacillus subtilis strain 2i was statistically optimized using mango kernels as substrate in submerged fermentation. Nine nutritional vari...
Molecular detection of honeybee pathogens in honey from a UK citizen science program [0.03%]
英国市民科学项目中蜂蜜中蜜蜂病原的分子检测
Michael J R Bennett,Lindsay K Newbold,Susheel Bhanu Busi et al.
Michael J R Bennett et al.
Fruits, legumes, oilseeds and nuts have a high dependency on insect pollination, of which the honey bee (Apis mellifera L.) is a critical contributor. However, declines in wild insect pollinators and managed honey bees have been widely repo...