Production, optimization and characterization of cellulase produced from Aspergillus niger in submerged fermentation through response surface methodology [0.03%]
响应面法培养产黑曲霉木聚糖酶及其性质研究
Kayynat Shoukat,Irfan Ahmad,Hafiz Abdullah Shakir et al.
Kayynat Shoukat et al.
Cellulase is considered as the most significant enzymes, widely studied owing to its extensive industrial applications and the increasing worldwide research focus on its production. In this research, mango peels were used as a source of car...
Fourier-transform infrared spectroscopy reliably differentiates Klebsiella pneumoniae outbreak isolates [0.03%]
傅里叶变换红外光谱可可靠地区分肺炎克雷伯氏菌暴发分离株
Miriam Cordovana,Markus Kostrzewa,Jürgen A Bohnert et al.
Miriam Cordovana et al.
International, multidrug-resistant (MDR) high-risk clonal Klebsiella pneumoniae lineages are a frequent cause of outbreaks, posing a serious public health threat. Tracking, understanding and containing such outbreaks remain a challenge for ...
Application of a two-level factorial design to investigate the effects of pH, temperature, nitrate concentration, and photoperiod on novel extracellular lipase activity of Nodosilinea sp. LGS3 [0.03%]
一种两水平因子设计法在pH值、温度、硝酸盐浓度和光照周期对LGS3菌株新型胞外脂肪酶活性影响中的应用
S Sangeetha,Lali Growther
S Sangeetha
The production of extracellular lipases from cyanobacteria offers a sustainable alternative to conventional enzyme sources for industrial and environmental applications. This study reports, for the first time, the production of a novel extr...
A streamlined protocol for high-quality RNA extraction from bread dough [0.03%]
一种从面团中高效提取高质量RNA的简化方案
Akira Nishimura,Shigeyuki Ota,Shota Isogai et al.
Akira Nishimura et al.
Bread dough is rich in polysaccharides and polyphenols, which hinder RNA extraction. We therefore established a streamlined workflow combining Fruit-mate homogenization, clarification, TRIzol extraction, high-salt precipitation, and activat...
Selective anti-adhesion and anti-biofilm action of D-phenylalanine chiral nanofilms against Staphylococcus aureus [0.03%]
D-苯丙氨酸手性纳米膜选择性地抑制金黄色葡萄球菌的附着和生物被膜形成作用
Xinglei Guo,Xiao Feng,Jianhao Zhang et al.
Xinglei Guo et al.
Biofilm formed by foodborne pathogens on contact surface are notoriously resistant to conventional disinfection, leading to equipment failure and persistent contamination. Therefore, modulating initial bacterial adhesion offers a promising ...
First report on ATR-FTIR and machine learning-based sputum profiling for TB detection: A compendium of potential biomarkers [0.03%]
基于ATR-FTIR和机器学习的TB检测首份痰液分析报告：潜在生物标志物汇总表
Zubia Shahid,Muhammad Tahir Hayat,Irshad Hussain et al.
Zubia Shahid et al.
Tuberculosis (TB) remains a significant global health issue, with millions of new cases reported annually and persistent limitations in conventional diagnostic approaches. This study is the first report that explores the use of Attenuated T...
A qPCR assay for the detection and quantification of Pseudomonas syringae pv. syringae in susceptible tissues of sweet cherry [0.03%]
甜樱桃感病组织中Ts菌致病变种检测与定量的qPCR方法研究
Yerko Lovera,Juan San Martin,Braulio Ruiz et al.
Yerko Lovera et al.
This study validated a qPCR assay for quantifying Pseudomonas syringae pv. syringae across three growth stages, using syrB gene as target and PVPP modification in extraction protocol, demonstrating high sensitivity and accuracy compared to ...
Different media affect the growth and antimicrobial minimum inhibitory concentrations of Mycoplasma gallisepticum and Mycoplasma synoviae [0.03%]
不同的培养基对鸡败血支原体和滑液囊支原体生长及最小抑制浓度的影响
Hossein Taiyari,Jalila Abu,Zunita Zakaria et al.
Hossein Taiyari et al.
The main pathogenic Mycoplasma spp. infecting chickens are Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). The MG and MS play an etiological role in chronic respiratory disease. One of the methods to control avian mycoplasmosis ...
Cost-effective immobilization of Chaetomium erraticum dextranase on c-MWCNT [0.03%]
芽孢菌淀粉酶固定化于c-MWTCNTs上的低成本技术
Barzan Ismael Ghafour,Yakup Aslan
Barzan Ismael Ghafour
In this study, dextranase enzyme (CED) from Chaetomium erraticum was immobilized on carboxylated multi-walled carbon nanotubes (c-MWCNTs) via adsorption. Immobilization conditions were optimized, and both free (FCED) and immobilized enzymes...
Improving the identification of pneumonia pathogens by using the real-time polymerase chain reaction (qPCR) alongside culture methods [0.03%]
联合应用实时定量 PCR 和培养法提高肺炎病原体的检出率
Nurul Izzaty Najwa Zahari,Jia Ying Wong,Siti Asma&#x; Hassan et al.
Nurul Izzaty Najwa Zahari et al.
Streptococcus pneumoniae, Haemophilus influenzae, and Klebsiella pneumoniae are among colonizers of the upper respiratory tract flora that can cause pneumonia upon invading the lungs. Molecular techniques have increasingly become the prefer...