A qPCR method for detection of 'Candidatus Liberibacter asiaticus': Optimization of selected assay parameters [0.03%]
Jonathan Phillips,Sohrab Bodaghi,José Á Sánchez Gómez et al.
Jonathan Phillips et al.
'Candidatus Liberibacter asiaticus' (CLas), the presumed causative agent of Huanglongbing (HLB, citrus greening), threatens citrus production worldwide. With no cure available, infected trees decline rapidly and entire groves can be lost. S...
Development and validation of a VirB12-based indirect ELISA for differentiating Brucella abortus infection from A19-ΔVirB12 vaccination [0.03%]
Ting Liang,Lu Ouyang,Jianrong Wu et al.
Ting Liang et al.
Brucellosis, a widespread zoonosis, requires reliable diagnostic tools capable of differentiating infected from vaccinated animals (DIVA), particularly with the emergence of DIVA-compatible vaccines such as Brucella abortus A19-ΔVirB12. Vi...
Hadrien Allain,Loona Koullen,Louis Coroller et al.
Hadrien Allain et al.
Spore-forming bacteria are key candidates for applications in biotechnology and biocontrol due to their resistance to physical and chemical stress and metabolic diversity. However, their recovery from soil is often hindered by limitations i...
Re-defining the culturing method for Pseudogymnoascus destructans incorporating viability detection [0.03%]
Braden D Mays,Riley F Bernard,Zachary P Roehrs et al.
Braden D Mays et al.
Pseudogymnoascus destructans (Pd) is a psychrophilic fungus whose genus is commonly found in soils of colder environments, including stable habitats such as caves and mines. The fungus causes the epizootic disease white-nose syndrome, which...
Impact of culture conditions on the performance of a device-standardized string test for detecting rmpA-positive Klebsiella pneumoniae [0.03%]
Koji Goto,Yuji Ito,Norihito Koyanagi et al.
Koji Goto et al.
Hypervirulent Klebsiella pneumoniae (hvKp) causes severe community-acquired infections globally. The hypermucoviscous hvKp phenotype is traditionally assessed using the manual string test, which exhibits limited reproducibility and suboptim...
LAMP-based detection of toxic Microcystis spp. as an early warning tool for water quality monitoring [0.03%]
Gabriela Martínez de la Escalera,Carolina Croci,Paola Scavone et al.
Gabriela Martínez de la Escalera et al.
Cyanobacteria of the Microcystis genus often form blooms in freshwater and estuarine environments, producing microcystin, a toxin harmful to animals and humans. The analysis of water samples for the detection of toxic Microcystis is usually...
An improved DiaRD MTB interferon-gamma release assay for the diagnosis of Mycobacterium Tuberculosis infections [0.03%]
Riza Durmaz,Sedat Vezir,Mustafa Ertek et al.
Riza Durmaz et al.
This study aimed to develop a DiaRD Mycobacterium tuberculosis interferon-gamma release assay (DiaRD MTB IGRA) and to evaluate its diagnostic performance in comparison with the QuantiFERON-TB Gold Plus (QFT-Plus) assay for the detection of ...
Optimized DNA extraction from fecal samples using non-stool kits to improve PCR detection of protozoan parasites: A focus on Blastocystis sp [0.03%]
Asma Guilane,Bachir Medrouh,Houria Zait et al.
Asma Guilane et al.
PCR-based detection of intestinal protozoa frequently faces challenges due to inadequate DNA recovery or the presence of inhibitors within fecal samples. Therefore, optimizing extraction protocols through pretreatment strategies is crucial ...
The simplified agar dilution method (SAD) for simple and accurate fosfomycin MIC testing of urinary Escherichia coli isolates [0.03%]
Shizhou Liang,Wenpin Cai,Mengquan Chen et al.
Shizhou Liang et al.
Background: Fosfomycin is crucial for treating multidrug-resistant urinary tract infections (UTIs), but its rational use depends on accurate MIC testing. The reference agar dilution (AD) method is labor-intensive and not ...
Mobile carbapenemases in Gram-negative bacilli: Modification of a simplified phenotypic method for routine laboratory detection [0.03%]
Ihorrana W Alflen,Sheila A B Nishiyama,Monica S F Mattos et al.
Ihorrana W Alflen et al.
Carbapenem resistance in Gram-negative bacilli (GNB) is a global health concern that requires accurate detection and effective treatment. Identification of isolates producing Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-β-l...