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Development and validation of a propidium monoazide-qPCR assay with matrix-matched calibration for selective quantification of viable Limosilactobacillus reuteri in multi-strain probiotic powders

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Accurate enumeration of viable probiotic cells in commercial products is essential for quality control, label compliance, and product development. Conventional plate counting is labour-intensive and primarily quantifies culturable cells, whereas quantitative P... ...