Deep Learning as a Compass for Industrial Biocatalysis: The Grase Framework Rewrites the Rules for Polyurethane Recycling [0.03%]
深度学习在工业生物催化的指南作用：Grase框架重塑聚氨酯回收规则
Lijun Wu,Lei Wang,Fan Wang et al.
Lijun Wu et al.
The GRASE deep-learning framework overcomes the enzyme activity-stability trade-off to discover AbPURase, a robust biocatalyst that efficiently depolymerizes polyurethane waste in harsh industrial conditions, enabling scalable, closed-loop ...
Autotransduction and Pseudotyping as a Key Limitations in Lentiviral Vector Production [0.03%]
慢病毒载体生产的自转导和伪型化的关键限制因素
Filip Walczak,Anna Szymkiewicz,Ilona Pacak et al.
Filip Walczak et al.
Lentiviral vectors are one of the important tools used in gene and cell therapy. Vector production involves a process known as pseudotyping, whereby glycoproteins originally derived from other viruses are incorporated into the envelope of t...
In Vivo Multi-Enzymatic Catalysis for Bioproduction of L-Lactic Acid from Sole Methanol [0.03%]
单一甲醇来源的L-乳酸体内多酶催化生物制造
Yunxia Li,Wei Yu,Lin Shan et al.
Yunxia Li et al.
Microbial biotransformation of methanol for lactate production presents a promising carbon-neutral strategy to mitigate "white pollution". However, this approach has been hindered by poor cell growth on methanol, largely due to the methanol...
Optimization Strategies of Polymeric Hydrogels and Decellularized Extracellular Matrices for Diabetic Chronic Wound Healing [0.03%]
用于糖尿病慢性伤口愈合的聚电解质水凝胶和脱细胞基质优化策略
Hongxi Liu,Langyu He,Xinyue Liu et al.
Hongxi Liu et al.
The treatment of chronic wounds in diabetes represents a significant challenge in the biomedical field. This review summarizes the application of three categories of biomaterials in this area: natural polymeric materials (gelatin, alginates...
Rational Design of a Carbonyl Reductase Yields a Robust Biocatalyst for Industrial Synthesis of (R)-3-Quinuclidinol [0.03%]
合理设计羰基还原酶用于手性药物中间体(R)-3-喹诺利醇的高效生物催化合成
Min Cao,Xiafeng Lu,Wen Zheng et al.
Min Cao et al.
(R)-3-quinuclidinol is a pivotal chiral synthon for pharmaceuticals such as talsaclidine, revatropate, and solifenacin. Conventional chemical synthesis routes, however, suffer from inherent drawbacks including inefficient racemic resolution...
Microprinted Epoxysilane Arrays for Conducting Microarray-Based Bioassays [0.03%]
微打印环氧硅阵列进行基于微阵列的生物分析
Roshan Tosh Aggarwal,Abdullah Abdelrahman,Monserrat Roceli Herver Cruz et al.
Roshan Tosh Aggarwal et al.
A common method of fabricating microarrays requires a microarray printer, which is unavailable to most laboratories. The printing process compromises bioactivity of the printed molecules. Here, we introduced an approach to micropatterning e...
Integrated Multi-Omics Analysis Elucidates the Metabolic Basis of Enhanced Echinocandin B Biosynthesis and Guides Targeted Engineering in Aspergillus nidulans [0.03%]
整合多组学分析阐明了增强棘白菌素B生物合成的代谢基础并在曲霉中指导目标工程化改造
Xiaozhang Yang,Ai-Ping Pang,Hui-Ling Chen et al.
Xiaozhang Yang et al.
Echinocandin B (ECB) serves as a crucial precursor for the antifungal drug anidulafungin, yet its production cost remains high due to low fermentation titers. We previously screened a highly efficient ECB producer Aspergillus nidulans ZJB16...
An Ad5-Based COVID-19 Vaccine Encoding SARS-CoV-2 Spike Glycoprotein Induces Measurable Antibody and Cytokine Responses in Mice [0.03%]
基于腺病毒5型载体的新型冠状病毒(SARS-CoV-2)棘突糖蛋白疫苗可诱导小鼠产生抗体和细胞因子免疫应答
Fulya Erendor,Fatih Uzer,Salih Sanlioglu
Fulya Erendor
The global SARS-CoV-2 pandemic has underlined the urgent need for effective vaccine platforms. Adenoviral vectors have gained attention due to their high transgene capacity, broad tissue tropism, and innate immunostimulatory properties. Thi...
Dianqi Yang,Mengyao Yuan,Zewei Lu et al.
Dianqi Yang et al.
Geranic acid, a high-value monoterpenoic acid with wide applications, faces supply constraints from the traditional plant extraction method. This study aims to develop an efficient microbial platform for its sustainable production. We engin...
Context-Specific Genome-Scale Metabolic Modeling to Unravel the Metabolic Rewiring in Human Diseases [0.03%]
基于场景的基因组范围代谢模型研究人类疾病中的代谢重组路径
Satyajit Beura,Dhruv Ripudeman Singh Bhadoriya,Shubham Dash et al.
Satyajit Beura et al.
Disruptions in cellular metabolism contributes to the development and progression of various diseases. A comprehensive understanding of these metabolic alterations is essential for developing targeted therapeutic interventions that can addr...