Photo-Crosslinking Aided Hierarchical Assembly of Recombinant Collagen Hydrogels for 3D Bio-Printing [0.03%]
光交联增强的重组胶原水凝胶分层组装用于三维生物打印技术
Chen Wang,Meng Zhang,Fei Xu
Chen Wang
Collagen is a critical structural and functional protein in extracellular matrix. Recombinant collagen provides key benefits such as virus-free safety, low immunogenicity, solubility, and quality consistency. However, it is still challenge ...
Adeno-Associated Virus Vector Mediated Gene Therapy: A Promising Approach to Transform Hypertrophic Cardiomyopathy Treatment [0.03%]
腺相关病毒载体介导的基因治疗：重塑肥厚型心肌病治疗的重要方法
Saif Siddiqui,Siddhi Bagwe Parab,Lokesh Kumar Bhatt
Saif Siddiqui
Hypertrophic cardiomyopathy (HCM) is a genetically heterogeneous myocardial disease that usually arises from mutations in key sarcomeric proteins, particularly MYBPC3 and MYH7. Current pharmacological treatments, such as myosin inhibitors, ...
3D Bioprinting for Tumor Microenvironment Reconstruction: Advances, Challenges, and Future Perspectives [0.03%]
肿瘤微环境重建的三维生物打印：进展、挑战和未来展望
Ling-Jun Yao,Pei-Lin Xie,Ting-Xuan Huang et al.
Ling-Jun Yao et al.
Cancer remains a major global health burden, and the disconnect between preclinical models and clinical outcomes continues to hinder progress in oncology. Conventional two-dimensional (2D) cell cultures fail to recapitulate the in vivo tumo...
Coated Bacterial Vaccine Platform Overcomes Weak Antigen Immunogenicity: A Functional Approach to GnRH-Based Immunocastration [0.03%]
包被细菌疫苗平台克服弱抗原免疫原性：基于GnRH的免疫去势功能性方法
Inés Harguindeguy,Matías H Assandri,María Antonieta Daza Millone et al.
Inés Harguindeguy et al.
Immunocastration, a nonsurgical strategy based on active immunization against gonadotropin-releasing hormone (GnRH), effectively suppresses steroidogenesis and spermatogenesis. However, peptide vaccines targeting poorly immunogenic antigens...
Base Editors for Engineering Industrial Microorganisms: Types, Applications, and Future Perspectives [0.03%]
基于碱基编辑的工业微生物工程：类型、应用和未来展望
Xurui Li,Shimin Wu,Pingfang Tian
Xurui Li
Base editing encompasses technologies that enable the direct conversion of one nucleotide base into another, typically by employing deaminases fused to programmable DNA- or RNA-binding scaffolds such as CRISPR-dCas9/nCas9. Deaminase-free ed...
Validation of an Automated Fluorescence- and Image-Based Viable Cell Counting Method for Fecal Microbiota Transplantation Drug Products [0.03%]
粪便微生物移植药物产品自动荧光和图像活菌计数法的验证
Timon Lang,Fedja Farowski,Jozef Al-Gousous et al.
Timon Lang et al.
Fecal microbiota transplantation (FMT) is an established treatment for recurrent Clostridioides difficile infection. As a drug product, FMT is subject to pharmaceutical quality standards, including accurate quantification of viable bacteria...
Attenuation of Triple Selection Markers to Enhance mAb Expression in Tyrosine-Based Selection Systems via an Inducible CRISPRi Platform [0.03%]
利用诱导型CRISPRi平台在基于酪氨酸的选择系统中减弱三重选择标记以增强mAb表达
Lei Cao,Liang Zhao,Yan Zhou et al.
Lei Cao et al.
Chinese hamster ovary (CHO) cells are the predominant host for monoclonal antibody (mAb) production, where the choice of selection system is critical. While metabolic selection systems are favored over antibiotic-based ones to avoid purific...
Systems-Level Metabolic Regulation Strategies of Xylose Metabolism in Saccharomyces cerevisiae [0.03%]
酿酒酵母木糖代谢的系统水平调控策略研究进展
Song Gao,Zhi-Hua Liu,Hao-Ran Zhang et al.
Song Gao et al.
As the second most abundant sugar in lignocellulosic hydrolysates, efficient xylose utilization is critical for achieving high carbon efficiency and economic viability of lignocellulosic fermentation. While heterologous pathway engineering ...
The Role of Exosomes in Bone Metastasis and Bone Tissue Engineering: Molecular Mechanisms and Therapeutic Opportunities [0.03%]
外泌体在骨转移和组织工程中的作用：分子机制与治疗机会
Asrin Emami,Iman Menbari Oskouie
Asrin Emami
Exosomes, nanoscale extracellular vesicles secreted by almost all cell types, have emerged as pivotal mediators of intercellular communication within both pathological and regenerative microenvironments. In bone metastasis, tumor-derived ex...
Harnessing Deep Learning Models for Guide RNA Optimization and Off-Target Prediction in CRISPR Systems [0.03%]
利用深度学习模型优化CRISPR系统中的向导RNA并预测脱靶效应
Muhammad Saeed,Muhammad Arham,Imran Zafar et al.
Muhammad Saeed et al.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-based genome and transcriptome editing technologies have emerged as powerful tools for therapeutic, agricultural, and industrial applications. However, their broader clinica...