Computational Fluid Dynamic Analysis of Fluid Motion and Volumetric Gas-Liquid Mass Transfer in Agitated Platelet Concentrate Storage [0.03%]
搅拌条件下血小板保存袋内流动及传质的计算流体动力学分析
Dean Pym,Amanda J Davies,Jessica O Williams et al.
Dean Pym et al.
Computational fluid dynamics (CFD) offers a powerful tool in characterizing the complex biophysical environment inducing by dynamic storage conditions, providing insights often beyond the reach of conventional experimental approaches. As ou...
From Liposomes to Virosomes: Evolution of Phospholipid Nanocarriers in Drug Delivery [0.03%]
从脂质体到病毒体：药物递送中磷脂纳米载体的发展演变
Kateryna Mykhailivna Doroshenko,Oleksandr Ivanovych Shevchenko
Kateryna Mykhailivna Doroshenko
Phospholipid-based nanocarriers are an adaptable and chemically tunable class of drug delivery systems that self-assemble into bilayered vesicles due to the amphiphilic nature of phospholipids. Phospholipid-based nanocarriers can encapsulat...
Sienna P Butterfield,Fay L Saunders,Robert J White
Sienna P Butterfield
Industrial production of biologics commonly involves the integration of transgenes into the genomes of host cells, such as Chinese hamster ovary (CHO) cells. A major determinant of productivity is the epigenetic control of the transgene pro...
Enhanced Lincomycin Titer Through Knockout of GntR Family Regulator SLCG_2790 Combined With Fermentation Optimization in an Industrial Strain of Streptomyces lincolnensis L-427 [0.03%]
通过基因敲除和发酵优化提高放线菌L-427链灰霉素产量
Yinxu Zhao,Feng Xu,Qinghai Shang et al.
Yinxu Zhao et al.
In this study, lincomycin A (Lin-A), a lincosamide antibiotic primarily synthesized by Streptomyces lincolnensis, was selected as a model to demonstrate a combined genetic regulation and process engineering strategy for production improveme...
A Growth-Coupled Evolutionary Strategy Enhances Heme Biosynthesis in Saccharomyces cerevisiae [0.03%]
一种与生长偶联的进化策略可提升酿酒酵母中铁 protoporphyrin IX 的生物合成能力
Seoyoon Bang,Eunbi Kim,Sehyeon Park et al.
Seoyoon Bang et al.
Enhancing the nutritional and sensory qualities of microbial single-cell proteins (SCPs) requires strategies to increase heme content in edible microorganisms. We adapted the Growth-Acceleration Targeting Evolution (GATE) platform, initiall...
Utilization of Miniature CRISPR-AsCas12f1 Nuclease for Efficient Genome Editing in Bacillus subtilis [0.03%]
利用微型CRISPR-AsCas12f1核酸酶高效编辑枯草芽孢杆菌基因组
Gexuan Pan,Li Wang,Haodong Zhu et al.
Gexuan Pan et al.
To address the limitations of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas)9 in Bacillus subtilis, such as low transformation efficiency and strong dependence on specific PAM sequences, this stud...
Mild Reduction-Mediated NK Cell Membrane Modification for ROS Scavenging Efficacy Overcome Immunosuppressive Tumor Microenvironment [0.03%]
基于NK细胞的膜修饰用于克服免疫抑制肿瘤微环境中的ROS清除效力
Kyung Mu Noh,Jaewon Park,Sungjun Kim et al.
Kyung Mu Noh et al.
Solid tumors are characterized by a metabolically dysregulated tumor microenvironment (TME) enriched with reactive oxygen species (ROS), which suppresses immune cell function. Natural killer (NK) cells are promising effectors in cancer immu...
Genome Amplification and Altered Transcriptome Aid in Survival and Enhanced Protein Secretion in Tunicamycin-Resistant CHOK1 Cells [0.03%]
基因组扩增和转录组改变可提高链霉亲和素耐药CHOK1细胞的存活率并增强其蛋白质分泌功能
Priya Mishra,Sarika Mehra
Priya Mishra
Chinese Hamster Ovary (CHO) cells are the predominant host for the production of biotherapeutics; however, there remains considerable potential to further enhance their cellular productivity. Adaptive laboratory evolution (ALE) combined wit...
Multi-Omics Reveals Impact of Hypoxia on Lactate Metabolic Switch and Monoclonal Antibody Production in CHO Fed-Batch Cultivation [0.03%]
多组学揭示低氧对CHO fed-batch培养中乳酸代谢转换及单克隆抗体生产的影响
Pei Yin,Weifeng Li,Wenting Zhao et al.
Pei Yin et al.
Oxygen supply is critical for mammalian cell culture. Insufficient oxygen supply or oxygen inhomogeneity in large-scale bioreactors often leads to attenuated process performance, such as reduced cell growth, viability, and productivity. How...
A 3D Bioprinted PDA/CSCA/PAM Hybrid Hydrogel Patch for Myocardial Infarction Healing [0.03%]
用于心肌梗死修复的3D生物打印PDA / CSCA / PAM混合水凝胶补片
Yiran Tang,Jie Xu,Wang Liu et al.
Yiran Tang et al.
Cardiovascular diseases are a leading global cause of mortality, with cardiac patches (CPs) emerging as a novel surgical treatment. This study used a combined decellularization method with sodium dodecyl sulfate (SDS), Triton X-100, and DNa...