Development and validation of a novel monocyte activation test with secreted luciferase-expressing cells [0.03%]
分泌型萤火虫荧光素酶细胞的新一代活化单核细胞检测方法的建立与验证
Teruaki Oku,Yusuke Ando,Tomohisa Nanao et al.
Teruaki Oku et al.
Pyrogen testing is essential for ensuring pharmaceutical product safety; however, current methods have limitations. The Bacterial Endotoxins Test cannot detect non-endotoxin pyrogens (NEPs), whereas the Rabbit Pyrogen Test was phased out fo...
Substrate specificity of an archaeal thioesterase from Thermococcus kodakarensis [0.03%]
来源于 Thermococcus kodakarensis 的古菌硫酯酶的底物特异性
Yu Su,Jianqiang Jin,Yuta Michimori et al.
Yu Su et al.
Thioesterases catalyze the hydrolysis of thioester bonds and are responsible for reactions such as the release of fatty acids from acyl-ACP in fatty acid biosynthesis. Although enzymes from bacteria and eukaryotes have been studied, identif...
Adhesion G protein-coupled receptor F5 (ADGRF5) mediates shear stress mechanotransduction in glomerular endothelial cells [0.03%]
黏附G蛋白偶联受体F5(ADGRF5)介导肾小球内皮细胞的剪切应力机械信号转导
Xiaoyang Wang,Masako Kato,Miki Nagase et al.
Xiaoyang Wang et al.
Adhesion G protein-coupled receptor F5 (ADGRF5) is widely expressed and is involved in various physiological and pathophysiological functions. ADGRF5 has been demonstrated to be expressed in glomerular endothelial cells and to maintain the ...
Individual Short-Chain Fatty Acids Differentially Reprogram Transcriptional States in Colorectal Cancer Cells [0.03%]
个体短链脂肪酸在结直肠癌细胞中差异性重编转录状态
Miho Seki,Karen Minowa,Satoshi Yamashita
Miho Seki
Short-chain fatty acids (SCFAs) produced by the gut microbiota contribute to intestinal function, immune responses, and host metabolism, yet how individual SCFAs shape intracellular gene expression states remains incompletely defined. Here,...
Cannabidiolic acid causes a defect in tail retraction of migrating MDA-MB-231 cells: Possible involvements of Rho-associated protein kinases (ROCKs) inhibition and accumulation of vinculin at the rear of migrating cells [0.03%]
大麻二酚酸导致迁移的MDA-MB-231细胞尾部收缩缺陷：Rho相关蛋白激酶（ROCK）抑制和后部vinculin积累的可能作用
Shuso Takeda,Masayo Hirao-Suzuki,Koki Kanameda et al.
Shuso Takeda et al.
We previously reported that cannabidiolic acid (CBDA), a major cannabinoid constituent of the fiber-type cannabis plant, abrogates the migration of highly aggressive human breast cancer MDA-MB-231 cells and activates the small GTPase RhoA b...
Ribosome display-derived CD207-binding peptides enhance humoral responses via Langerhans cell-targeted antigen delivery [0.03%]
一种针对CD207的肽类分子可靶向朗格汉斯细胞并增强体液免疫应答（利用核糖体显示技术筛选获得）
Mina Yokoyama,Yoshirou Kawaguchi,Rie Wakabayashi et al.
Mina Yokoyama et al.
Langerhans cells (LCs) are specialized antigen-presenting cells residing in the epidermis. LCs express the C-type lectin receptor CD207, which mediates antigen uptake and internalization. Because epidermal LCs encounter antigens at the skin...
Zinc-mediated structural and functional regulation of ERp44 and ERGIC-53 in protein quality control [0.03%]
锌介导的ERp44和ERGIC-53在蛋白质质量控制中的结构与功能调节
Satoshi Watanabe,Kenji Inaba
Satoshi Watanabe
Zinc ions (Zn2+) are essential trace metal ions in the human body. Intracellular Zn2+ levels are tightly regulated by two metal transporter families: ZIPs, which mediate Zn2+ influx into the cytosol, and ZnTs, which export Zn2+ from the cyt...
Endothelial RhoA is essential for embryonic and postnatal vascular development by regulating KLF2-Notch1 signaling [0.03%]
内皮RhoA通过调节KLF2-Notch1信号通路在胚胎和出生后血管发育中起关键作用
Wondwossen Wale Tesega,Akio Shimizu,Taha Hussain et al.
Wondwossen Wale Tesega et al.
The small GTPase RhoA has diverse cellular functions, including in actin fiber reorganization. However, its role in angiogenesis remains elusive. To reveal this, we generated mice with endothelium-specific constitutive or tamoxifen-inducibl...
A two-tiered epigenetic mechanism ensures imprint stability after fertilization: insights from the Igf2/H19 locus [0.03%]
受精后的双层表观遗传机制确保印记的稳定性：来自Igf2 / H19位点的见解
Hitomi Matsuzaki,Teruhito Ishihara,Keiji Tanimoto
Hitomi Matsuzaki
Genomic imprinting is a crucial epigenetic mechanism directing parent-of-origin-specific gene expression in mammals, and it is regulated by allele-specific DNA methylation at imprinting control regions (ICRs). In this canonical imprinting m...
Targeting the CCL2-CCR2 axis in cancer: insights from in vitro screening and humanized mice [0.03%]
靶向癌症中的CCL2-CCR2轴：来自体外筛选和人源化小鼠的见解
Yanjun Chen,Midori Shimada
Yanjun Chen
An immunosuppressive tumor microenvironment that facilitates cancer progression is an important challenge in combating the disease. The CCL2-CCR2 axis is a primary facilitator in this process, facilitating the recruitment of CCR2-positive m...