Syouichi Katayama,Miwako Sakota,Atsushi Morii
Syouichi Katayama
Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine protein kinase highly expressed in the brain, and mutations in its gene cause CDKL5 deficiency disorder (CDD). Although reports on CDKL5 substrate proteins are increasing, the pho...
Phosphatidylinositol 3,4-bisphosphate Emerges as a Lipid Mediator Linking Oxidative Stress to DNA Damage [0.03%]
Yuto Kikuchi,Hiroaki Kajiho,Junya Hasegawa et al.
Yuto Kikuchi et al.
Reactive oxygen species function as physiological signaling molecules but, when excessive, cause oxidative stress that damages DNA. Using quantitative phosphoinositide profiling by regioisomer-resolving mass spectrometry, we identified phos...
Hirotatsu Imai,Akio Yamashita
Hirotatsu Imai
Cytosolic 80S ribosomes have long been considered a uniform translation apparatus, with the only distinction being that they exist as either monosomes or polysomes. However, emerging evidence has revealed that monosomes and polysomes exhibi...
DDX5: A Versatile RNA Helicase in Cancer and Cell Fate Determination-Linking c-Myc and JAK2 V617F Signaling to Emerging Therapeutics [0.03%]
DDX5：一种多功能RNA解旋酶在癌症和细胞命运决定中的作用——连接c-Myc和JAK2 V617F信号通路与新兴疗法
Kenji Tago,Megumi Funakoshi-Tago
Kenji Tago
Traditionally regarded as a DEAD-box RNA helicase involved in RNA metabolism, DDX5 (also known as p68) has emerged as a signal-responsive regulator that integrates RNA processing with transcriptional control, thereby influencing cell fate d...
Mitigating Alkaline Instability Induced by Tyrosine-Tyrosine Repulsion in an FcγRIIIa-Binding Protein Through Phenylalanine Substitution [0.03%]
通过苯丙氨酸替代缓解酪胺酸相互排斥引发的fcγriiiA结合蛋白的碱性不稳定性
Rio Okuda,Yuki Tokunaga,Satoru Nagatoishi et al.
Rio Okuda et al.
Proteins are essential components in biotechnological and biopharmaceutical applications; however, their structural instability under alkaline conditions presents significant limitations. High-pH environments, such as chromatographic clean-...
Recurrent Mutations in Protein Tyrosine Phosphatase Receptor Type Kappa (PTPRK) in Depressed-Type Colorectal Carcinomas [0.03%]
抑素型结直肠癌中蛋白酪氨酸磷酸酶受体Kappa(PTPRK)的复发性突变
Masayuki Tojo,Keiko Shinjo,Fumiharu Ohka et al.
Masayuki Tojo et al.
Genetic and epigenetic alterations in colorectal cancer (CRC) have been extensively investigated. Small depressed-type colorectal tumors (DCs) constitute a highly invasive CRC subtype. We characterized the mutational profiles of 22 DC sampl...
Improving purity and apparent thermal stability of single-chain Fv antibodies by heat treatment in a soluble E. coli cytoplasmic expression system [0.03%]
通过热处理改善单链Fv抗体在可溶性大肠杆菌胞质表达系统中的纯度和表观热稳定性
Sai De,Yoshihiro Kobashigawa,Kyo Okazaki et al.
Sai De et al.
Single-chain variable fragment (scFv) antibodies outperform conventional IgG antibodies due to their lower molecular weight, reduced immunogenicity, rapid clearance, and efficient bacterial expression. However, it remains difficult to achie...
Observation of redistribution for local conformational dynamics in cross-reactive antibody design [0.03%]
用于交叉反应性抗体设计的局部构象动力学再分布的观察
Yoshiki Yasuda,Satoru Nagatoishi,Jiei Sasaki et al.
Yoshiki Yasuda et al.
Antibody engineering is often achieved through laborious mutagenesis and screening. However, the physicochemical basis of cross-reactivity-enhancing mutations remains unclear. We computationally redesigned the severe acute respiratory syndr...
Severe hyperosmotic stress causes dysregulation of replication-dependent histone mRNA expression and induces DNA replication stress [0.03%]
严重的高渗应激会导致依赖复制的组蛋白mrna表达失调并诱导dna复制压力
Yasunao Kamikawa,Zuqian Wu,Kazunori Imaizumi et al.
Yasunao Kamikawa et al.
The expression of replication-dependent (RD) histones is tightly regulated by several pathways, including transcription and mRNA processing. However, the detailed mechanisms underlying the regulation of specific RD histone genes remain to b...
Quantification of L- and D-enantiomers of proline, trans-4-hydroxyproline, and cis-4-hydroxyproline in natural samples using differential labeling with 12C- and 13C6-dabsyl chloride, chiral separation, and liquid chromatography-mass spectrometry [0.03%]
使用12C-and-13C6-二苯基氨基甲酰氯差示标记、手性分离和液相色谱质谱法测定天然样品中脯氨酸、反式-4-羟基脯氨酸和顺式-4-羟基脯氨酸的L-和D-对映异构体数量
Bhisma Rai,Tetsuo Ishida
Bhisma Rai
L-Proline (L-Pro) and trans-4-hydroxy-L-proline (t-4-HPL) are major components of collagen and plant cell wall proteins. During their breakdown, free L-Pro and t-4-HPL are produced abundantly and play unique biological roles through complex...