Surface loops may provide additional function necessary for enzyme-to-pseudoenzyme transition in the fungal metallocarboxypeptidase family [0.03%]
表面环路可能为真菌金属羧肽酶家族中的酶到伪酶转变提供必要功能
Zhiheng Zhao,Faith C Kaluba,Peter J Lyons
Zhiheng Zhao
The transition from enzyme to pseudoenzyme is thought to begin with moonlighting enzymes that have gained nonenzymatic functions. Subsequent gene duplication events allow for the separation of enzyme and pseudoenzyme function. We explored t...
In vitro affinity maturation of a single-chain antibody against thyroxine based on computer-aided design [0.03%]
基于计算机辅助设计的单链抗体甲状腺素体外亲和力成熟优化
Meilun Chen,Yijie Liu,Zheng Wei et al.
Meilun Chen et al.
Thyroxine (T4) plays a crucial role in regulating various physiological functions in the human body. As a key biomolecule for molecular recognition, single-chain variable fragments (scFvs) offer advantages, such as small molecular size, hig...
The ALS-associated E425K mutation uncouples DNAJC7 from the Hsp70 chaperone cycle [0.03%]
ALS关联的E425K突变使DNAJC7脱离Hsp70分子伴侣循环
Bar Elmaleh,Ofrah Faust,Rina Rosenzweig
Bar Elmaleh
DNAJC7, a member of the J-domain protein (JDP/Hsp40) family, plays a key role in protein homeostasis by regulating Hsp70 activity and preventing protein aggregation. Mutations in DNAJC7 have been linked to amyotrophic lateral sclerosis (ALS...
The FEBS Journal in 2026: Safeguarding scientific integrity amid the rise of the machines [0.03%]
在机器崛起的时代保障科学诚信——《FEBS杂志》2026年展望
Seamus J Martin
Seamus J Martin
The FEBS Journal publishes primary papers and reviews relating to the molecules and mechanisms underpinning biological processes. Editor-in-Chief Seamus Martin discusses some of the challenges posed by the increasing use of generative AI to...
Laura Morette,Fanny Marceau,Sandrine Mathiot et al.
Laura Morette et al.
Glutathione transferases (GSTs) constitute a widespread superfamily of multifunctional enzymes with roles in cellular detoxification and secondary metabolism. We report that the poorly characterized Iota-class enzymes (GSTIs) are mainly fou...
Deubiquitinase inhibitors: Targeting SARS-CoV-2 papain-like protease with antiviral efficacy in a murine model [0.03%]
特异性靶向冠状病毒去泛素酶抑制剂体内抗病毒活性评价
Shweta Choudhary,Sanketkumar Nehul,Santhosh Kambaiah Nagaraj et al.
Shweta Choudhary et al.
SARS-CoV-2 papain-like protease (PLpro) is a key antiviral target as it plays a dual role in viral replication and modulation of innate immune responses by deubiquitinating or deISGylating host proteins. Therefore, therapeutically targeting...
A miRNA screen reveals a transcriptional network controlling the initiation of mammary organoids [0.03%]
长非编码RNA和microRNAs调节小鼠乳腺类器官发生的转录网络筛查
Jacques Robert,Lena Gosset,Efstathios-Iason Vlachavas et al.
Jacques Robert et al.
Organoid formation is driven by poorly understood intrinsic cellular properties and transcriptional programs that govern plasticity and differentiation. Deciphering these regulatory networks is essential for understanding normal tissue home...
Mitochondrial uncoupler BAM15 ameliorates liver lipid metabolism disorders by activating the AMPK pathway [0.03%]
线粒体解偶联剂BAM15通过激活AMPK信号通路改善肝脏脂代谢紊乱
Zunhai Liu,Wentao Wang,Simeng Wang et al.
Zunhai Liu et al.
N5,N6-bis(2-Fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine (BAM15) is a recently identified mitochondrial uncoupler with antitumor, anti-inflammatory, antioxidant and antiobesity properties. Although it has been shown that BAM15...
Capillary differences with age and muscle fiber type are attenuated by accounting for fiber shape [0.03%]
通过校正纤维形状后年龄和肌纤维类型间的血管差异被削弱
Jonathan F Frydenholm,Cecilie J L Bechshøft,Michael Kjaer et al.
Jonathan F Frydenholm et al.
Age-related declines in skeletal muscle capillarization are well-documented, yet commonly used indices, such as the capillary-to-fiber perimeter exchange index (CFPE), assume uniform myofiber shape. Because fiber size and shape changes with...