Side-chain to backbone correlations from solid-state NMR of perdeuterated proteins through combined excitation and long-range magnetization transfers [0.03%]
结合激励和长程磁化转移观测去uter代蛋白质固态NMR侧链与主链之间的相关谱
Rasmus Linser
Rasmus Linser
Proteins with excessive deuteration give access to proton detected solid-state NMR spectra of extraordinary resolution and sensitivity. The high spectral quality achieved after partial proton back-exchange has been shown to start a new era ...
Using the water signal to detect invisible exchanging protons in the catalytic triad of a serine protease [0.03%]
利用水信号探测丝氨酸蛋白酶催化三联体中交换的质子
Carolyn B Lauzon,Peter van Zijl,James T Stivers
Carolyn B Lauzon
Chemical Exchange Saturation Transfer (CEST) is an MRI approach that can indirectly detect exchange broadened protons that are invisible in traditional NMR spectra. We modified the CEST pulse sequence for use on high-resolution spectrometer...
Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion [0.03%]
33 ku 尿素2 索状体C-端结构域的NMR谱学研究
Birgit Habenstein,Christian Wasmer,Luc Bousset et al.
Birgit Habenstein et al.
We present the de novo resonance assignments for the crystalline 33 kDa C-terminal domain of the Ure2 prion using an optimized set of five 3D solid-state NMR spectra. We obtained, using a single uniformly (13)C, (15)N labeled protein sample...
Engineering [Ln(DPA)3] 3- binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions [0.03%]
工程[Ln(DPA)3]3-结合位点在蛋白质中：一种广泛适用的蛋白质与镧系离子标记的方法
Xinying Jia,Hiromasa Yagi,Xun-Cheng Su et al.
Xinying Jia et al.
Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, however, are tethered to the prot...
Aleksandr B Sahakyan,Wim F Vranken,Andrea Cavalli et al.
Aleksandr B Sahakyan et al.
Protein methyl groups have recently been the subject of much attention in NMR spectroscopy because of the opportunities that they provide to obtain information about the structure and dynamics of proteins and protein complexes. With the adv...
Optimization of NMR spectroscopy of encapsulated proteins dissolved in low viscosity fluids [0.03%]
低粘度液体中包封蛋白质的NMR谱学优化研究方法
Nathaniel V Nucci,Bryan S Marques,Sabrina Bédard et al.
Nathaniel V Nucci et al.
Comprehensive application of solution NMR spectroscopy to studies of macromolecules remains fundamentally limited by the molecular rotational correlation time. For proteins, molecules larger than 30 kDa require complex experimental methods,...
A segmental labeling strategy for unambiguous determination of domain-domain interactions of large multi-domain proteins [0.03%]
用于大型多域蛋白无歧义确定域-域相互作用的片段式标记策略
Jianglei Chen,Jianjun Wang
Jianglei Chen
NMR structural determination of large multi-domain proteins is a challenging task due to significant spectral overlap with a particular difficulty in unambiguous identification of domain-domain interactions. Segmental labeling is a NMR stra...
A general Monte Carlo/simulated annealing algorithm for resonance assignment in NMR of uniformly labeled biopolymers [0.03%]
用于均匀标记生物高分子的核磁共振谱峰归属的通用型蒙特卡罗/模拟退火算法
Kan-Nian Hu,Wei Qiang,Robert Tycko
Kan-Nian Hu
We describe a general computational approach to site-specific resonance assignments in multidimensional NMR studies of uniformly (15)N,(13)C-labeled biopolymers, based on a simple Monte Carlo/simulated annealing (MCSA) algorithm contained i...
Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data [0.03%]
利用RDC定义的主链及无TOCSY数据进行蛋白质侧链共振分配和NOE分配
Jianyang Zeng,Pei Zhou,Bruce Randall Donald
Jianyang Zeng
One bottleneck in NMR structure determination lies in the laborious and time-consuming process of side-chain resonance and NOE assignments. Compared to the well-studied backbone resonance assignment problem, automated side-chain resonance a...
Overexpression of a homogeneous oligosaccharide with 13C labeling by genetically engineered yeast strain [0.03%]
通过基因工程酵母过量表达具有同质寡糖13C标记的化合物
Yukiko Kamiya,Sayoko Yamamoto,Yasunori Chiba et al.
Yukiko Kamiya et al.
This report describes a novel method for overexpression of (13)C-labeled oligosaccharides using genetically engineered Saccharomyces cerevisiae cells, in which a homogeneous high-mannose-type oligosaccharide accumulates because of deletions...